Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury
(1) Following acute spinal cord injury, progesterone modulates several molecules essential for motoneuron function, although the morphological substrates for these effects are unknown. (2) The present study analyzed morphological changes in motoneurons distal to the lesion site from rats with or wit...
Guardado en:
| Publicado: |
2009
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v29_n1_p27_Gonzalez http://hdl.handle.net/20.500.12110/paper_02724340_v29_n1_p27_Gonzalez |
| Aporte de: |
| id |
paper:paper_02724340_v29_n1_p27_Gonzalez |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_02724340_v29_n1_p27_Gonzalez2023-06-08T15:25:06Z Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury Chromatolysis Electron microscopy MAP2 Motoneuron Neuroprotection Progesterone Spinal cord injury microtubule associated protein 2 microtubule protein progesterone animal cell animal experiment animal model article cell ultrastructure controlled study cytoplasm cytoskeleton dendritic spine electron microscopy immunohistochemistry male motoneuron neuromodulation neuroprotection Nissl substance nonhuman nucleus accumbens perikaryon polysome priority journal rat rough endoplasmic reticulum spinal cord injury therapy effect treatment duration treatment outcome treatment response Acute Disease Animals Cell Nucleolus Immunohistochemistry Male Microtubule-Associated Proteins Motor Neurons Neurons Progesterone Rats Rats, Sprague-Dawley Spinal Cord Injuries Rattus (1) Following acute spinal cord injury, progesterone modulates several molecules essential for motoneuron function, although the morphological substrates for these effects are unknown. (2) The present study analyzed morphological changes in motoneurons distal to the lesion site from rats with or without progesterone treatment. We employed electron microscopy to study changes in nucleus and cytoplasm and immunohistochemistry for the microtubule-associated protein 2 (MAP2) for changes in cytoskeleton. (3) After spinal cord injury, the nucleoplasm appeared more finely dispersed resulting in reduced electron opacity and the nucleus adopted an eccentric position. Changes of perikarya included dissolution of Nissl bodies and dissociation of polyribosomes (chromatolysis). After progesterone treatment for 3 days, the deafferented motoneurons now presented a clumped nucleoplasm, a better-preserved rough endoplasmic reticulum and absence of chromatolysis. Progesterone partially prevented development of nuclear eccentricity. Whereas 50% of injured motoneurons showed nuclear eccentricity, only 16% presented this phenotype after receiving progesterone. Additionally, injured rats showed reduced immunostaining for MAP2 in dendrites, pointing to cytoskeleton abnormalities, whereas progesterone treatment attenuated the injury-induced loss of MAP2. (4) Our data indicated that progesterone maintained in part neuronal ultrastructure, attenuated chromatolysis, and preclude the loss of MAP2, suggesting a protective effect during the early phases of spinal cord injury. © 2008 Springer Science+Business Media, LLC. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v29_n1_p27_Gonzalez http://hdl.handle.net/20.500.12110/paper_02724340_v29_n1_p27_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chromatolysis Electron microscopy MAP2 Motoneuron Neuroprotection Progesterone Spinal cord injury microtubule associated protein 2 microtubule protein progesterone animal cell animal experiment animal model article cell ultrastructure controlled study cytoplasm cytoskeleton dendritic spine electron microscopy immunohistochemistry male motoneuron neuromodulation neuroprotection Nissl substance nonhuman nucleus accumbens perikaryon polysome priority journal rat rough endoplasmic reticulum spinal cord injury therapy effect treatment duration treatment outcome treatment response Acute Disease Animals Cell Nucleolus Immunohistochemistry Male Microtubule-Associated Proteins Motor Neurons Neurons Progesterone Rats Rats, Sprague-Dawley Spinal Cord Injuries Rattus |
| spellingShingle |
Chromatolysis Electron microscopy MAP2 Motoneuron Neuroprotection Progesterone Spinal cord injury microtubule associated protein 2 microtubule protein progesterone animal cell animal experiment animal model article cell ultrastructure controlled study cytoplasm cytoskeleton dendritic spine electron microscopy immunohistochemistry male motoneuron neuromodulation neuroprotection Nissl substance nonhuman nucleus accumbens perikaryon polysome priority journal rat rough endoplasmic reticulum spinal cord injury therapy effect treatment duration treatment outcome treatment response Acute Disease Animals Cell Nucleolus Immunohistochemistry Male Microtubule-Associated Proteins Motor Neurons Neurons Progesterone Rats Rats, Sprague-Dawley Spinal Cord Injuries Rattus Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury |
| topic_facet |
Chromatolysis Electron microscopy MAP2 Motoneuron Neuroprotection Progesterone Spinal cord injury microtubule associated protein 2 microtubule protein progesterone animal cell animal experiment animal model article cell ultrastructure controlled study cytoplasm cytoskeleton dendritic spine electron microscopy immunohistochemistry male motoneuron neuromodulation neuroprotection Nissl substance nonhuman nucleus accumbens perikaryon polysome priority journal rat rough endoplasmic reticulum spinal cord injury therapy effect treatment duration treatment outcome treatment response Acute Disease Animals Cell Nucleolus Immunohistochemistry Male Microtubule-Associated Proteins Motor Neurons Neurons Progesterone Rats Rats, Sprague-Dawley Spinal Cord Injuries Rattus |
| description |
(1) Following acute spinal cord injury, progesterone modulates several molecules essential for motoneuron function, although the morphological substrates for these effects are unknown. (2) The present study analyzed morphological changes in motoneurons distal to the lesion site from rats with or without progesterone treatment. We employed electron microscopy to study changes in nucleus and cytoplasm and immunohistochemistry for the microtubule-associated protein 2 (MAP2) for changes in cytoskeleton. (3) After spinal cord injury, the nucleoplasm appeared more finely dispersed resulting in reduced electron opacity and the nucleus adopted an eccentric position. Changes of perikarya included dissolution of Nissl bodies and dissociation of polyribosomes (chromatolysis). After progesterone treatment for 3 days, the deafferented motoneurons now presented a clumped nucleoplasm, a better-preserved rough endoplasmic reticulum and absence of chromatolysis. Progesterone partially prevented development of nuclear eccentricity. Whereas 50% of injured motoneurons showed nuclear eccentricity, only 16% presented this phenotype after receiving progesterone. Additionally, injured rats showed reduced immunostaining for MAP2 in dendrites, pointing to cytoskeleton abnormalities, whereas progesterone treatment attenuated the injury-induced loss of MAP2. (4) Our data indicated that progesterone maintained in part neuronal ultrastructure, attenuated chromatolysis, and preclude the loss of MAP2, suggesting a protective effect during the early phases of spinal cord injury. © 2008 Springer Science+Business Media, LLC. |
| title |
Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury |
| title_short |
Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury |
| title_full |
Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury |
| title_fullStr |
Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury |
| title_full_unstemmed |
Progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (MAP2) in bats with acute spinal cord injury |
| title_sort |
progesterone effects on neuronal ultrastructure and expression of microtubule-associated protein 2 (map2) in bats with acute spinal cord injury |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v29_n1_p27_Gonzalez http://hdl.handle.net/20.500.12110/paper_02724340_v29_n1_p27_Gonzalez |
| _version_ |
1768542644531625984 |