Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse
1. Synthesis of oxytocin (OT) and arginine-vasopressin (AVP) is increased in induced models of Type I diabetes, such as the streptozotocin model. However, these parameters have not yet been evaluated in spontaneous models, such as the nonobese diabetic mouse (NOD). Therefore, we studied in the magno...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v21_n1_p15_Saravia http://hdl.handle.net/20.500.12110/paper_02724340_v21_n1_p15_Saravia |
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paper:paper_02724340_v21_n1_p15_Saravia2023-06-08T15:25:05Z Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse Diabetes mellitus Nonobese diabetic (NOD) mouse Oxytocin Paraventricular nucleus Vasopressin argipressin messenger RNA neuropeptide oxytocin potassium protein sodium urea animal experiment animal model article blood chemistry controlled study experimental diabetes mellitus female giant cell hematocrit hypernatremia immunocytochemistry immunoreactivity in situ hybridization mouse nonhuman peptide synthesis priority journal protein blood level protein expression signal transduction stress thalamus midline nucleus Animals Diabetes Mellitus, Type 1 Female Immunohistochemistry In Situ Hybridization Mice Mice, Inbred C57BL Mice, Inbred NOD Oxytocin Paraventricular Hypothalamic Nucleus RNA, Messenger Specific Pathogen-Free Organisms Vasopressins 1. Synthesis of oxytocin (OT) and arginine-vasopressin (AVP) is increased in induced models of Type I diabetes, such as the streptozotocin model. However, these parameters have not yet been evaluated in spontaneous models, such as the nonobese diabetic mouse (NOD). Therefore, we studied in the magnocellular cells of the paraventricular nucleus (PVN) of nondiabetic and diabetic 16-week-old female NOD mice and control C57B1/6 mice, the immunocytochemistry of OT and AVP peptides and their mRNA expression, using nonisotopic in situ hybridization (ISH). 2. In nondiabetic and diabetic NOD female mice, the number of OT- and AVP-immunoreactive cells were similar to those of the controls, whereas immunoreaction intensity was significantly higher for both peptides in diabetic NOD as compared with nondiabetic NOD and control C57B1/6 mice. 3. ISH analysis showed that the number of OT mRNA-containing cells was in the same range in the three groups, whereas higher number of AVP mRNA expressing cells was found in diabetic NOD mice. However, the intensity of hybridization signal was also higher for both OT and AVP mRNA in the diabetic group as compared with nondiabetic NOD and control mice. 4. Blood chemistry demonstrated that haematrocrit, total plasma proteins, urea, sodium, and potassium were within normal limits in diabetic mice. Thus, NOD mice were neither hypernatremic nor dehydrated. 5. We suggest that upregulation of OT and AVP reflects a high-stress condition in the NOD mice. Diabetes may affect neuropeptide-producing cells of the PVN, with the increased AVP and OT playing a deleterious role on the outcome of the disease. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v21_n1_p15_Saravia http://hdl.handle.net/20.500.12110/paper_02724340_v21_n1_p15_Saravia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Diabetes mellitus Nonobese diabetic (NOD) mouse Oxytocin Paraventricular nucleus Vasopressin argipressin messenger RNA neuropeptide oxytocin potassium protein sodium urea animal experiment animal model article blood chemistry controlled study experimental diabetes mellitus female giant cell hematocrit hypernatremia immunocytochemistry immunoreactivity in situ hybridization mouse nonhuman peptide synthesis priority journal protein blood level protein expression signal transduction stress thalamus midline nucleus Animals Diabetes Mellitus, Type 1 Female Immunohistochemistry In Situ Hybridization Mice Mice, Inbred C57BL Mice, Inbred NOD Oxytocin Paraventricular Hypothalamic Nucleus RNA, Messenger Specific Pathogen-Free Organisms Vasopressins |
| spellingShingle |
Diabetes mellitus Nonobese diabetic (NOD) mouse Oxytocin Paraventricular nucleus Vasopressin argipressin messenger RNA neuropeptide oxytocin potassium protein sodium urea animal experiment animal model article blood chemistry controlled study experimental diabetes mellitus female giant cell hematocrit hypernatremia immunocytochemistry immunoreactivity in situ hybridization mouse nonhuman peptide synthesis priority journal protein blood level protein expression signal transduction stress thalamus midline nucleus Animals Diabetes Mellitus, Type 1 Female Immunohistochemistry In Situ Hybridization Mice Mice, Inbred C57BL Mice, Inbred NOD Oxytocin Paraventricular Hypothalamic Nucleus RNA, Messenger Specific Pathogen-Free Organisms Vasopressins Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse |
| topic_facet |
Diabetes mellitus Nonobese diabetic (NOD) mouse Oxytocin Paraventricular nucleus Vasopressin argipressin messenger RNA neuropeptide oxytocin potassium protein sodium urea animal experiment animal model article blood chemistry controlled study experimental diabetes mellitus female giant cell hematocrit hypernatremia immunocytochemistry immunoreactivity in situ hybridization mouse nonhuman peptide synthesis priority journal protein blood level protein expression signal transduction stress thalamus midline nucleus Animals Diabetes Mellitus, Type 1 Female Immunohistochemistry In Situ Hybridization Mice Mice, Inbred C57BL Mice, Inbred NOD Oxytocin Paraventricular Hypothalamic Nucleus RNA, Messenger Specific Pathogen-Free Organisms Vasopressins |
| description |
1. Synthesis of oxytocin (OT) and arginine-vasopressin (AVP) is increased in induced models of Type I diabetes, such as the streptozotocin model. However, these parameters have not yet been evaluated in spontaneous models, such as the nonobese diabetic mouse (NOD). Therefore, we studied in the magnocellular cells of the paraventricular nucleus (PVN) of nondiabetic and diabetic 16-week-old female NOD mice and control C57B1/6 mice, the immunocytochemistry of OT and AVP peptides and their mRNA expression, using nonisotopic in situ hybridization (ISH). 2. In nondiabetic and diabetic NOD female mice, the number of OT- and AVP-immunoreactive cells were similar to those of the controls, whereas immunoreaction intensity was significantly higher for both peptides in diabetic NOD as compared with nondiabetic NOD and control C57B1/6 mice. 3. ISH analysis showed that the number of OT mRNA-containing cells was in the same range in the three groups, whereas higher number of AVP mRNA expressing cells was found in diabetic NOD mice. However, the intensity of hybridization signal was also higher for both OT and AVP mRNA in the diabetic group as compared with nondiabetic NOD and control mice. 4. Blood chemistry demonstrated that haematrocrit, total plasma proteins, urea, sodium, and potassium were within normal limits in diabetic mice. Thus, NOD mice were neither hypernatremic nor dehydrated. 5. We suggest that upregulation of OT and AVP reflects a high-stress condition in the NOD mice. Diabetes may affect neuropeptide-producing cells of the PVN, with the increased AVP and OT playing a deleterious role on the outcome of the disease. |
| title |
Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse |
| title_short |
Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse |
| title_full |
Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse |
| title_fullStr |
Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse |
| title_full_unstemmed |
Diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of Type I diabetes, the nonobese diabetic (NOD) mouse |
| title_sort |
diabetes increases the expression of hypothalamic neuropeptides in a spontaneous model of type i diabetes, the nonobese diabetic (nod) mouse |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02724340_v21_n1_p15_Saravia http://hdl.handle.net/20.500.12110/paper_02724340_v21_n1_p15_Saravia |
| _version_ |
1768544639968608256 |