TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH
This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat splenocytes, a single TRH receptor mRNA band of about 3.8...
Guardado en:
| Autor principal: | |
|---|---|
| Publicado: |
1995
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02719142_v15_n5_p242_Raiden http://hdl.handle.net/20.500.12110/paper_02719142_v15_n5_p242_Raiden |
| Aporte de: |
| id |
paper:paper_02719142_v15_n5_p242_Raiden |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_02719142_v15_n5_p242_Raiden2023-06-08T15:25:00Z TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH Labeur, Marta Susana immuneneuroendocrine interactions lymphocyte activation TRH TRH-receptor messenger rna protirelin protirelin receptor animal cell animal experiment article controlled study dna synthesis female immunostimulation intraperitoneal drug administration lymphocyte activation nonhuman priority journal rat Animal Antibodies Blotting, Northern Cells, Cultured DNA Female Gene Expression Human Leukocytes, Mononuclear Lymphocyte Activation Lymphocytes Protirelin Rats Rats, Wistar Receptors, Thyrotropin-Releasing Hormone RNA, Messenger Spleen Stimulation, Chemical Support, Non-U.S. Gov't This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated splenocytes when TRH (10-6 M-10-12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin. © 1995 Plenum Publishing Corporation. Fil:Labeur, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02719142_v15_n5_p242_Raiden http://hdl.handle.net/20.500.12110/paper_02719142_v15_n5_p242_Raiden |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
immuneneuroendocrine interactions lymphocyte activation TRH TRH-receptor messenger rna protirelin protirelin receptor animal cell animal experiment article controlled study dna synthesis female immunostimulation intraperitoneal drug administration lymphocyte activation nonhuman priority journal rat Animal Antibodies Blotting, Northern Cells, Cultured DNA Female Gene Expression Human Leukocytes, Mononuclear Lymphocyte Activation Lymphocytes Protirelin Rats Rats, Wistar Receptors, Thyrotropin-Releasing Hormone RNA, Messenger Spleen Stimulation, Chemical Support, Non-U.S. Gov't |
| spellingShingle |
immuneneuroendocrine interactions lymphocyte activation TRH TRH-receptor messenger rna protirelin protirelin receptor animal cell animal experiment article controlled study dna synthesis female immunostimulation intraperitoneal drug administration lymphocyte activation nonhuman priority journal rat Animal Antibodies Blotting, Northern Cells, Cultured DNA Female Gene Expression Human Leukocytes, Mononuclear Lymphocyte Activation Lymphocytes Protirelin Rats Rats, Wistar Receptors, Thyrotropin-Releasing Hormone RNA, Messenger Spleen Stimulation, Chemical Support, Non-U.S. Gov't Labeur, Marta Susana TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH |
| topic_facet |
immuneneuroendocrine interactions lymphocyte activation TRH TRH-receptor messenger rna protirelin protirelin receptor animal cell animal experiment article controlled study dna synthesis female immunostimulation intraperitoneal drug administration lymphocyte activation nonhuman priority journal rat Animal Antibodies Blotting, Northern Cells, Cultured DNA Female Gene Expression Human Leukocytes, Mononuclear Lymphocyte Activation Lymphocytes Protirelin Rats Rats, Wistar Receptors, Thyrotropin-Releasing Hormone RNA, Messenger Spleen Stimulation, Chemical Support, Non-U.S. Gov't |
| description |
This work examined whether (1) immune cells express thyrotrophin releasing hormone (TRH) receptor mRNA and (2) TRH modulates lymphocyte activation. By Northern blot of RNA extracted from human peripheral blood mononuclear cells (PBMC) and rat splenocytes, a single TRH receptor mRNA band of about 3.8 kb (identical to that obtained from pituitary cells) was obtained, under both basal and stimulated conditions. A significant increase in DNA synthesis was observed in phytohemagglutinin-stimulated PBMC and concanavalin A (Con A) stimulated splenocytes when TRH (10-6 M-10-12 M) was added. After 5, 30, 60, 180 min and 24 h of TRH administration in vivo, a significant increase in the rat splenocyte proliferative response to Con A was observed. In vivo administration of anti-rat TSH antibody (1/1000) blocked the increase observed after 30 min of TRH administration on the Con A stimulated splenocyte response. TRH possess immunostimulatory functions directly via its receptor and indirectly via release of other immunostimulatory factors such as thyrotrophin. © 1995 Plenum Publishing Corporation. |
| author |
Labeur, Marta Susana |
| author_facet |
Labeur, Marta Susana |
| author_sort |
Labeur, Marta Susana |
| title |
TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH |
| title_short |
TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH |
| title_full |
TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH |
| title_fullStr |
TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH |
| title_full_unstemmed |
TRH receptor on immune cells:In vitro and in Vivo stimulation of human lymphocyte and rat splenocyte DNA synthesis by TRH |
| title_sort |
trh receptor on immune cells:in vitro and in vivo stimulation of human lymphocyte and rat splenocyte dna synthesis by trh |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02719142_v15_n5_p242_Raiden http://hdl.handle.net/20.500.12110/paper_02719142_v15_n5_p242_Raiden |
| work_keys_str_mv |
AT labeurmartasusana trhreceptoronimmunecellsinvitroandinvivostimulationofhumanlymphocyteandratsplenocytednasynthesisbytrh |
| _version_ |
1768542787586752512 |