Developmental changes in calcium channel types mediating central synaptic transmission
Multiple types of high-voltage-activated Ca2+ channels trigger neurotransmitter release at the mammalian central synapse. Among them, the ω-conotoxin GVIA-sensitive N-type channels and the ω-Aga-IVA-sensitive P/Q- type channels mediate fast synaptic transmission. However, at most central synapses, i...
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2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v20_n1_p59_Iwasaki http://hdl.handle.net/20.500.12110/paper_02706474_v20_n1_p59_Iwasaki |
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paper:paper_02706474_v20_n1_p59_Iwasaki2023-06-08T15:24:42Z Developmental changes in calcium channel types mediating central synaptic transmission Central synapse N-type calcium channels P/Q-type calcium channels Postnatal development Slice Transmitter release calcium channel neurotransmitter omega conotoxin animal tissue article brain cortex calcium current controlled study GABAergic system nerve ending neurotransmitter release nonhuman postnatal development priority journal rat spinal cord dorsal horn synapse synaptic transmission 2-Amino-5-phosphonovalerate 6-Cyano-7-nitroquinoxaline-2,3-dione Age Factors Animals Brain Chemistry Calcium Channel Blockers Calcium Channels, N-Type Calcium Channels, R-Type Central Nervous System Evoked Potentials Excitatory Amino Acid Antagonists Excitatory Postsynaptic Potentials gamma-Aminobutyric Acid Glycine Agents omega-Agatoxin IVA omega-Conotoxins Organ Culture Techniques Rats Rats, Wistar Strychnine Synaptic Transmission Multiple types of high-voltage-activated Ca2+ channels trigger neurotransmitter release at the mammalian central synapse. Among them, the ω-conotoxin GVIA-sensitive N-type channels and the ω-Aga-IVA-sensitive P/Q- type channels mediate fast synaptic transmission. However, at most central synapses, it is not known whether the contributions of different Ca2+ channel types to synaptic transmission remain stable throughout postnatal development. We have addressed this question by testing type-specific Ca2+ channel blockers at developing central synapses. Our results indicate that N- type channels contribute to thalamic and cerebellar IPSCs only transiently during early postnatal period and P/Q-type channels predominantly mediate mature synaptic transmission, as we reported previously at the brainstem auditory synapse formed by the calyx of Held. In fact, Ca2+ currents directly recorded from the auditory calyceal presynaptic terminal were identified as N-, P/Q-, and R-types at postnatal day 7 (P7) to P10 but became predominantly P/Q-type at P13. In contrast to thalamic and cerebellar IPSCs and brainstem auditory EPSCs, N-type Ca2+ channels persistently contribute to cerebral cortical EPSCs and spinal IPSCs throughout postnatal months. Thus, in adult animals, synaptic transmission is predominantly mediated by P/Q-type channels at a subset of synapses and mediated synergistically by multiple types of Ca2+ channels at other synapses. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v20_n1_p59_Iwasaki http://hdl.handle.net/20.500.12110/paper_02706474_v20_n1_p59_Iwasaki |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Central synapse N-type calcium channels P/Q-type calcium channels Postnatal development Slice Transmitter release calcium channel neurotransmitter omega conotoxin animal tissue article brain cortex calcium current controlled study GABAergic system nerve ending neurotransmitter release nonhuman postnatal development priority journal rat spinal cord dorsal horn synapse synaptic transmission 2-Amino-5-phosphonovalerate 6-Cyano-7-nitroquinoxaline-2,3-dione Age Factors Animals Brain Chemistry Calcium Channel Blockers Calcium Channels, N-Type Calcium Channels, R-Type Central Nervous System Evoked Potentials Excitatory Amino Acid Antagonists Excitatory Postsynaptic Potentials gamma-Aminobutyric Acid Glycine Agents omega-Agatoxin IVA omega-Conotoxins Organ Culture Techniques Rats Rats, Wistar Strychnine Synaptic Transmission |
| spellingShingle |
Central synapse N-type calcium channels P/Q-type calcium channels Postnatal development Slice Transmitter release calcium channel neurotransmitter omega conotoxin animal tissue article brain cortex calcium current controlled study GABAergic system nerve ending neurotransmitter release nonhuman postnatal development priority journal rat spinal cord dorsal horn synapse synaptic transmission 2-Amino-5-phosphonovalerate 6-Cyano-7-nitroquinoxaline-2,3-dione Age Factors Animals Brain Chemistry Calcium Channel Blockers Calcium Channels, N-Type Calcium Channels, R-Type Central Nervous System Evoked Potentials Excitatory Amino Acid Antagonists Excitatory Postsynaptic Potentials gamma-Aminobutyric Acid Glycine Agents omega-Agatoxin IVA omega-Conotoxins Organ Culture Techniques Rats Rats, Wistar Strychnine Synaptic Transmission Developmental changes in calcium channel types mediating central synaptic transmission |
| topic_facet |
Central synapse N-type calcium channels P/Q-type calcium channels Postnatal development Slice Transmitter release calcium channel neurotransmitter omega conotoxin animal tissue article brain cortex calcium current controlled study GABAergic system nerve ending neurotransmitter release nonhuman postnatal development priority journal rat spinal cord dorsal horn synapse synaptic transmission 2-Amino-5-phosphonovalerate 6-Cyano-7-nitroquinoxaline-2,3-dione Age Factors Animals Brain Chemistry Calcium Channel Blockers Calcium Channels, N-Type Calcium Channels, R-Type Central Nervous System Evoked Potentials Excitatory Amino Acid Antagonists Excitatory Postsynaptic Potentials gamma-Aminobutyric Acid Glycine Agents omega-Agatoxin IVA omega-Conotoxins Organ Culture Techniques Rats Rats, Wistar Strychnine Synaptic Transmission |
| description |
Multiple types of high-voltage-activated Ca2+ channels trigger neurotransmitter release at the mammalian central synapse. Among them, the ω-conotoxin GVIA-sensitive N-type channels and the ω-Aga-IVA-sensitive P/Q- type channels mediate fast synaptic transmission. However, at most central synapses, it is not known whether the contributions of different Ca2+ channel types to synaptic transmission remain stable throughout postnatal development. We have addressed this question by testing type-specific Ca2+ channel blockers at developing central synapses. Our results indicate that N- type channels contribute to thalamic and cerebellar IPSCs only transiently during early postnatal period and P/Q-type channels predominantly mediate mature synaptic transmission, as we reported previously at the brainstem auditory synapse formed by the calyx of Held. In fact, Ca2+ currents directly recorded from the auditory calyceal presynaptic terminal were identified as N-, P/Q-, and R-types at postnatal day 7 (P7) to P10 but became predominantly P/Q-type at P13. In contrast to thalamic and cerebellar IPSCs and brainstem auditory EPSCs, N-type Ca2+ channels persistently contribute to cerebral cortical EPSCs and spinal IPSCs throughout postnatal months. Thus, in adult animals, synaptic transmission is predominantly mediated by P/Q-type channels at a subset of synapses and mediated synergistically by multiple types of Ca2+ channels at other synapses. |
| title |
Developmental changes in calcium channel types mediating central synaptic transmission |
| title_short |
Developmental changes in calcium channel types mediating central synaptic transmission |
| title_full |
Developmental changes in calcium channel types mediating central synaptic transmission |
| title_fullStr |
Developmental changes in calcium channel types mediating central synaptic transmission |
| title_full_unstemmed |
Developmental changes in calcium channel types mediating central synaptic transmission |
| title_sort |
developmental changes in calcium channel types mediating central synaptic transmission |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02706474_v20_n1_p59_Iwasaki http://hdl.handle.net/20.500.12110/paper_02706474_v20_n1_p59_Iwasaki |
| _version_ |
1768545416167555072 |