Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry
Methods for the analysis of phenformin and its metabolite by high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESIMS) are developed. The effects of pH, buffer concentration and prop...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02693879_v10_n4_p155_Llambias http://hdl.handle.net/20.500.12110/paper_02693879_v10_n4_p155_Llambias |
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paper:paper_02693879_v10_n4_p155_Llambias2023-06-08T15:24:21Z Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry Llambías, Elena Blanca Cecilia phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia Methods for the analysis of phenformin and its metabolite by high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESIMS) are developed. The effects of pH, buffer concentration and proportion of organic modifier on the retention of the compounds in HPLC have been studied. The optimum condition was used for the separation and identification of phenformin and its metabolite in microsomal metabolism by HPLC-ESIMS. A simple CE method is also described for the separation of these compounds. Optimum incubation conditions and cofactor requirements for the formation of 4-hydroxyphenformin by microsomal preparations of rat liver were determined. A linear response in the formation of product was found with increasing concentrations of protein and up to 15 min incubation. High concentrations of phenformin inhibited its metabolite formation, and K(m) was 4 μM. Fil:Llambias, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02693879_v10_n4_p155_Llambias http://hdl.handle.net/20.500.12110/paper_02693879_v10_n4_p155_Llambias |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia |
| spellingShingle |
phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia Llambías, Elena Blanca Cecilia Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry |
| topic_facet |
phenformin animal cell article capillary electrophoresis concentration response controlled study drug metabolism high performance liquid chromatography incubation time ionization liver microsome mass spectrometry metabolic inhibition metabolite nonhuman ph rat Acetates Animals Buffers Chromatography, High Pressure Liquid Electrophoresis, Capillary Female Hydrogen-Ion Concentration Hypoglycemic Agents Mass Spectrometry Metformin Methanol Microsomes, Liver Online Systems Osmolar Concentration Phenformin Rats Spectrophotometry, Ultraviolet Time Factors Animalia |
| description |
Methods for the analysis of phenformin and its metabolite by high-performance liquid chromatography (HPLC), capillary electrophoresis (CE) and high-performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-ESIMS) are developed. The effects of pH, buffer concentration and proportion of organic modifier on the retention of the compounds in HPLC have been studied. The optimum condition was used for the separation and identification of phenformin and its metabolite in microsomal metabolism by HPLC-ESIMS. A simple CE method is also described for the separation of these compounds. Optimum incubation conditions and cofactor requirements for the formation of 4-hydroxyphenformin by microsomal preparations of rat liver were determined. A linear response in the formation of product was found with increasing concentrations of protein and up to 15 min incubation. High concentrations of phenformin inhibited its metabolite formation, and K(m) was 4 μM. |
| author |
Llambías, Elena Blanca Cecilia |
| author_facet |
Llambías, Elena Blanca Cecilia |
| author_sort |
Llambías, Elena Blanca Cecilia |
| title |
Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry |
| title_short |
Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry |
| title_full |
Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry |
| title_fullStr |
Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry |
| title_full_unstemmed |
Study of phenformin metabolism in rat liver microsomes by HPLC, CE and on-line HPLC-electrospray ionization mass spectrometry |
| title_sort |
study of phenformin metabolism in rat liver microsomes by hplc, ce and on-line hplc-electrospray ionization mass spectrometry |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02693879_v10_n4_p155_Llambias http://hdl.handle.net/20.500.12110/paper_02693879_v10_n4_p155_Llambias |
| work_keys_str_mv |
AT llambiaselenablancacecilia studyofphenforminmetabolisminratlivermicrosomesbyhplcceandonlinehplcelectrosprayionizationmassspectrometry |
| _version_ |
1768545140465467392 |