Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways
STUDY QUESTION Do human trophoblast cells modulate neutrophil extracellular trap (NET) formation, reactive oxygen species (ROS) synthesis and neutrophil apoptosis through mechanisms involving vasoactive intestinal peptide (VIP)? SUMMARY ANSWER Trophoblast cells inhibited NET formation and ROS synthe...
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2017
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v32_n1_p55_Calo http://hdl.handle.net/20.500.12110/paper_02681161_v32_n1_p55_Calo |
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paper:paper_02681161_v32_n1_p55_Calo |
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dspace |
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Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
neutrophil apoptosis neutrophil extracellular traps neutrophils phagocytosis of apoptotic cells trophoblast cells vasoactive intestinal peptide DNA leukocyte elastase lipopolysaccharide phorbol 13 acetate 12 myristate reactive oxygen metabolite small interfering RNA vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist reactive oxygen metabolite vasoactive intestinal polypeptide apoptosis Article conditioned medium confocal microscopy controlled study enzyme activity enzyme linked immunosorbent assay extracellular trap first trimester pregnancy flow cytometry fluorescence microscopy genetic transfection human human cell in vitro study monocyte observational study phagocyte phagocytosis trophoblast apoptosis cell line drug effects extracellular trap female metabolism neutrophil pharmacology physiology pregnancy signal transduction trophoblast Apoptosis Cell Line Culture Media, Conditioned Extracellular Traps Female Humans Neutrophils Phagocytosis Pregnancy Reactive Oxygen Species Signal Transduction Trophoblasts Vasoactive Intestinal Peptide |
| spellingShingle |
neutrophil apoptosis neutrophil extracellular traps neutrophils phagocytosis of apoptotic cells trophoblast cells vasoactive intestinal peptide DNA leukocyte elastase lipopolysaccharide phorbol 13 acetate 12 myristate reactive oxygen metabolite small interfering RNA vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist reactive oxygen metabolite vasoactive intestinal polypeptide apoptosis Article conditioned medium confocal microscopy controlled study enzyme activity enzyme linked immunosorbent assay extracellular trap first trimester pregnancy flow cytometry fluorescence microscopy genetic transfection human human cell in vitro study monocyte observational study phagocyte phagocytosis trophoblast apoptosis cell line drug effects extracellular trap female metabolism neutrophil pharmacology physiology pregnancy signal transduction trophoblast Apoptosis Cell Line Culture Media, Conditioned Extracellular Traps Female Humans Neutrophils Phagocytosis Pregnancy Reactive Oxygen Species Signal Transduction Trophoblasts Vasoactive Intestinal Peptide Vota, Daiana Marina Ramhorst, Rosanna Elizabeth Trevani, Analía S. Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways |
| topic_facet |
neutrophil apoptosis neutrophil extracellular traps neutrophils phagocytosis of apoptotic cells trophoblast cells vasoactive intestinal peptide DNA leukocyte elastase lipopolysaccharide phorbol 13 acetate 12 myristate reactive oxygen metabolite small interfering RNA vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist reactive oxygen metabolite vasoactive intestinal polypeptide apoptosis Article conditioned medium confocal microscopy controlled study enzyme activity enzyme linked immunosorbent assay extracellular trap first trimester pregnancy flow cytometry fluorescence microscopy genetic transfection human human cell in vitro study monocyte observational study phagocyte phagocytosis trophoblast apoptosis cell line drug effects extracellular trap female metabolism neutrophil pharmacology physiology pregnancy signal transduction trophoblast Apoptosis Cell Line Culture Media, Conditioned Extracellular Traps Female Humans Neutrophils Phagocytosis Pregnancy Reactive Oxygen Species Signal Transduction Trophoblasts Vasoactive Intestinal Peptide |
| description |
STUDY QUESTION Do human trophoblast cells modulate neutrophil extracellular trap (NET) formation, reactive oxygen species (ROS) synthesis and neutrophil apoptosis through mechanisms involving vasoactive intestinal peptide (VIP)? SUMMARY ANSWER Trophoblast cells inhibited NET formation and ROS synthesis and enhanced neutrophil apoptosis through VIP-mediated pathways in a model of maternal-placental interaction. WHAT IS KNOWN ALREADY Immune homeostasis maintenance at the maternal-placental interface is mostly coordinated by trophoblast cells. Neutrophil activation and NET formation increases in pregnancies complicated by exacerbated pro-inflammatory responses. VIP has anti-inflammatory and immunosuppressant effects and is synthesized by trophoblast cells. STUDY DESIGN, SIZE, DURATION This is a laboratory-based observational study that sampled circulating neutrophils from 50 healthy volunteers to explore their response in vitro to factors derived from human trophoblast cells. PARTICIPANTS/MATERIALS, SETTING, METHODS Peripheral blood neutrophils were isolated from healthy volunteers and tested in vitro with first trimester trophoblast cell line (Swan-71 and HTR8) conditioned media (CM) or with VIP. The effect of VIP and trophoblast CM on NET formation was assessed by co-localization of elastase and DNA by confocal microscopy, DNA release and elastase activity measurement. Neutrophil apoptosis was determined by flow cytometry or fluorescence microscopy. ROS formation was assessed by flow cytometry with a fluorescent probe. VIP silencing was performed by siRNA transfection. For phagocytosis of apoptotic neutrophils, autologous monocytes were sampled, and engulfment and cytokines were assessed by flow cytometry and ELISA. MAIN RESULTS AND THE ROLE OF CHANCE Trophoblast CM and 10 nM VIP promoted neutrophil deactivation by preventing phorbol myristate acetate-induced NET formation and ROS synthesis while they increased neutrophil spontaneous apoptosis and reversed the anti-apoptotic effect of lipopolysaccharide (all P < 0.05 versus control). The effects of trophoblast CM were prevented by a VIP antagonist or when VIP knocked-down trophoblast cells were used (P < 0.05 versus control). Neutrophils driven to apoptosis by trophoblast CM could be rapidly engulfed by monocytes without increasing IL-12 production. LARGE SCALE DATA Not applicable. LIMITATIONS, REASONS FOR CAUTION The mechanisms of neutrophil deactivation by trophoblast VIP are based on the results obtained with neutrophils drawn from peripheral blood of healthy individuals interacting with trophoblast cell lines in vitro. These studies were designed to investigate biological processes at the cellular and molecular level; therefore, they have the limitations of studies in vitro and it is not possible to ascertain if these mechanisms operate similarly in vivo. We tested 50 neutrophil samples from healthy volunteers that have a normal variability in their responses. Cell lines derived from human trophoblast were used, and we cannot rule out a differential behavior of trophoblast cells in contact with neutrophils in vivo. WIDER IMPLICATIONS OF THE FINDINGS Results presented here are consistent with an active mechanism through which neutrophils in contact with trophoblast cells would be deactivated and silently cleared by decidual macrophages throughout pregnancy. They support a novel immunomodulatory role of trophoblast VIP on neutrophils at the placenta, providing new clues for pharmacological targeting of immune and trophoblast cells in pregnancy complications associated with exacerbated inflammation. © The Author 2016. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. |
| author |
Vota, Daiana Marina Ramhorst, Rosanna Elizabeth Trevani, Analía S. |
| author_facet |
Vota, Daiana Marina Ramhorst, Rosanna Elizabeth Trevani, Analía S. |
| author_sort |
Vota, Daiana Marina |
| title |
Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways |
| title_short |
Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways |
| title_full |
Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways |
| title_fullStr |
Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways |
| title_full_unstemmed |
Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways |
| title_sort |
trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways |
| publishDate |
2017 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v32_n1_p55_Calo http://hdl.handle.net/20.500.12110/paper_02681161_v32_n1_p55_Calo |
| work_keys_str_mv |
AT votadaianamarina trophoblastcellsinhibitneutrophilextracellulartrapformationandenhanceapoptosisthroughvasoactiveintestinalpeptidemediatedpathways AT ramhorstrosannaelizabeth trophoblastcellsinhibitneutrophilextracellulartrapformationandenhanceapoptosisthroughvasoactiveintestinalpeptidemediatedpathways AT trevanianalias trophoblastcellsinhibitneutrophilextracellulartrapformationandenhanceapoptosisthroughvasoactiveintestinalpeptidemediatedpathways |
| _version_ |
1768544775546339328 |
| spelling |
paper:paper_02681161_v32_n1_p55_Calo2023-06-08T15:24:10Z Trophoblast cells inhibit neutrophil extracellular trap formation and enhance apoptosis through vasoactive intestinal peptide-mediated pathways Vota, Daiana Marina Ramhorst, Rosanna Elizabeth Trevani, Analía S. neutrophil apoptosis neutrophil extracellular traps neutrophils phagocytosis of apoptotic cells trophoblast cells vasoactive intestinal peptide DNA leukocyte elastase lipopolysaccharide phorbol 13 acetate 12 myristate reactive oxygen metabolite small interfering RNA vasoactive intestinal polypeptide vasoactive intestinal polypeptide antagonist reactive oxygen metabolite vasoactive intestinal polypeptide apoptosis Article conditioned medium confocal microscopy controlled study enzyme activity enzyme linked immunosorbent assay extracellular trap first trimester pregnancy flow cytometry fluorescence microscopy genetic transfection human human cell in vitro study monocyte observational study phagocyte phagocytosis trophoblast apoptosis cell line drug effects extracellular trap female metabolism neutrophil pharmacology physiology pregnancy signal transduction trophoblast Apoptosis Cell Line Culture Media, Conditioned Extracellular Traps Female Humans Neutrophils Phagocytosis Pregnancy Reactive Oxygen Species Signal Transduction Trophoblasts Vasoactive Intestinal Peptide STUDY QUESTION Do human trophoblast cells modulate neutrophil extracellular trap (NET) formation, reactive oxygen species (ROS) synthesis and neutrophil apoptosis through mechanisms involving vasoactive intestinal peptide (VIP)? SUMMARY ANSWER Trophoblast cells inhibited NET formation and ROS synthesis and enhanced neutrophil apoptosis through VIP-mediated pathways in a model of maternal-placental interaction. WHAT IS KNOWN ALREADY Immune homeostasis maintenance at the maternal-placental interface is mostly coordinated by trophoblast cells. Neutrophil activation and NET formation increases in pregnancies complicated by exacerbated pro-inflammatory responses. VIP has anti-inflammatory and immunosuppressant effects and is synthesized by trophoblast cells. STUDY DESIGN, SIZE, DURATION This is a laboratory-based observational study that sampled circulating neutrophils from 50 healthy volunteers to explore their response in vitro to factors derived from human trophoblast cells. PARTICIPANTS/MATERIALS, SETTING, METHODS Peripheral blood neutrophils were isolated from healthy volunteers and tested in vitro with first trimester trophoblast cell line (Swan-71 and HTR8) conditioned media (CM) or with VIP. The effect of VIP and trophoblast CM on NET formation was assessed by co-localization of elastase and DNA by confocal microscopy, DNA release and elastase activity measurement. Neutrophil apoptosis was determined by flow cytometry or fluorescence microscopy. ROS formation was assessed by flow cytometry with a fluorescent probe. VIP silencing was performed by siRNA transfection. For phagocytosis of apoptotic neutrophils, autologous monocytes were sampled, and engulfment and cytokines were assessed by flow cytometry and ELISA. MAIN RESULTS AND THE ROLE OF CHANCE Trophoblast CM and 10 nM VIP promoted neutrophil deactivation by preventing phorbol myristate acetate-induced NET formation and ROS synthesis while they increased neutrophil spontaneous apoptosis and reversed the anti-apoptotic effect of lipopolysaccharide (all P < 0.05 versus control). The effects of trophoblast CM were prevented by a VIP antagonist or when VIP knocked-down trophoblast cells were used (P < 0.05 versus control). Neutrophils driven to apoptosis by trophoblast CM could be rapidly engulfed by monocytes without increasing IL-12 production. LARGE SCALE DATA Not applicable. LIMITATIONS, REASONS FOR CAUTION The mechanisms of neutrophil deactivation by trophoblast VIP are based on the results obtained with neutrophils drawn from peripheral blood of healthy individuals interacting with trophoblast cell lines in vitro. These studies were designed to investigate biological processes at the cellular and molecular level; therefore, they have the limitations of studies in vitro and it is not possible to ascertain if these mechanisms operate similarly in vivo. We tested 50 neutrophil samples from healthy volunteers that have a normal variability in their responses. Cell lines derived from human trophoblast were used, and we cannot rule out a differential behavior of trophoblast cells in contact with neutrophils in vivo. WIDER IMPLICATIONS OF THE FINDINGS Results presented here are consistent with an active mechanism through which neutrophils in contact with trophoblast cells would be deactivated and silently cleared by decidual macrophages throughout pregnancy. They support a novel immunomodulatory role of trophoblast VIP on neutrophils at the placenta, providing new clues for pharmacological targeting of immune and trophoblast cells in pregnancy complications associated with exacerbated inflammation. © The Author 2016. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. Fil:Vota, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ramhorst, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Trevani, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v32_n1_p55_Calo http://hdl.handle.net/20.500.12110/paper_02681161_v32_n1_p55_Calo |