A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation
BACKGROUND: Successful implantation is followed by a local pro-inflammatory and Th1 response, subsequently controlled by Th2. Regulated upon activation, normal T cell expressed and secreted (RANTES) promotes a Th1 response and is implicated as a physiologic tolerogenic factor; therefore, we studied...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v24_n1_p166_Fraccaroli http://hdl.handle.net/20.500.12110/paper_02681161_v24_n1_p166_Fraccaroli |
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paper:paper_02681161_v24_n1_p166_Fraccaroli2023-06-08T15:24:09Z A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation Chemokines Recurrent spontaneous miscarriages T cell Tolerance and pregnancy CD4 antigen chemokine gamma interferon interleukin 12p70 leukemia inhibitory factor lipocortin 5 nitrite progesterone RANTES transcription factor FOXP3 tumor necrosis factor alpha apoptosis article CD3+ T lymphocyte CD4+ CD25+ Foxp3+ T lymphocyte cell death cell proliferation cell selection cell strain Swan 7I cell survival clinical article coculture enzyme linked immunosorbent assay female fluorescence activated cell sorting human human cell immune response kinetics leukemia leukocyte peripheral blood mononuclear cell regulatory T lymphocyte spontaneous abortion T lymphocyte activation trophoblast Western blotting BACKGROUND: Successful implantation is followed by a local pro-inflammatory and Th1 response, subsequently controlled by Th2. Regulated upon activation, normal T cell expressed and secreted (RANTES) promotes a Th1 response and is implicated as a physiologic tolerogenic factor; therefore, we studied its potential role in the trophoblast-maternal leukocyte dialog. METHODS: We performed co-cultures of immortalized trophoblast cell line (Swan 71) and peripheral blood mononuclear cells (PBMCs) from fertile women (n = 23) or with recurrent spontaneous abortions (n = 18, RSA). After 24 and 48 h, supernatant and cells were analyzed by enzyme-linked immunosorbent assay, fluorescence-activated cell sorting, Western blot and apoptosis assay. To investigate the physiological effects at peripheral level, we co-cultured maternal and paternal PBMCs with conditioned media from Swan cells and progesterone. RESULTS: Following interaction of maternal PBMCs and trophoblast cells, RANTES production increased (P < 0.05) and was accompanied by low levels of interferon γ, interleukin-12 p70 and high levels of tumor necrosis factor-α, nitrites and leukemia-inhibitory factor. RANTES production resulted in elevated apoptosis of potentially deleterious maternal CD3+ lymphocytes, accompanied by a decrease in the proliferative maternal response. During fetal-maternal dialog, the anti-RANTES antibody significantly reduced the frequency of CD4+CD25+Foxp3+ cells (P < 0.05) and was associated with trophoblast cell survival. However, co-cultures of Swan cells and RSA-PBMCs displayed a differential RANTES kinetics, lower levels of regulatory T cells (Tregs) and CD3+annexin-V+cells, accompanied by higher levels of apoptotic trophoblast cells. CONCLUSIONS: RANTES promotes an adequate pro-implantatory microenvironment that influences trophoblast cell survival and modulates the balance of maternal Treg/T effector lymphocytes in favor of maternal tolerance. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v24_n1_p166_Fraccaroli http://hdl.handle.net/20.500.12110/paper_02681161_v24_n1_p166_Fraccaroli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chemokines Recurrent spontaneous miscarriages T cell Tolerance and pregnancy CD4 antigen chemokine gamma interferon interleukin 12p70 leukemia inhibitory factor lipocortin 5 nitrite progesterone RANTES transcription factor FOXP3 tumor necrosis factor alpha apoptosis article CD3+ T lymphocyte CD4+ CD25+ Foxp3+ T lymphocyte cell death cell proliferation cell selection cell strain Swan 7I cell survival clinical article coculture enzyme linked immunosorbent assay female fluorescence activated cell sorting human human cell immune response kinetics leukemia leukocyte peripheral blood mononuclear cell regulatory T lymphocyte spontaneous abortion T lymphocyte activation trophoblast Western blotting |
| spellingShingle |
Chemokines Recurrent spontaneous miscarriages T cell Tolerance and pregnancy CD4 antigen chemokine gamma interferon interleukin 12p70 leukemia inhibitory factor lipocortin 5 nitrite progesterone RANTES transcription factor FOXP3 tumor necrosis factor alpha apoptosis article CD3+ T lymphocyte CD4+ CD25+ Foxp3+ T lymphocyte cell death cell proliferation cell selection cell strain Swan 7I cell survival clinical article coculture enzyme linked immunosorbent assay female fluorescence activated cell sorting human human cell immune response kinetics leukemia leukocyte peripheral blood mononuclear cell regulatory T lymphocyte spontaneous abortion T lymphocyte activation trophoblast Western blotting A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation |
| topic_facet |
Chemokines Recurrent spontaneous miscarriages T cell Tolerance and pregnancy CD4 antigen chemokine gamma interferon interleukin 12p70 leukemia inhibitory factor lipocortin 5 nitrite progesterone RANTES transcription factor FOXP3 tumor necrosis factor alpha apoptosis article CD3+ T lymphocyte CD4+ CD25+ Foxp3+ T lymphocyte cell death cell proliferation cell selection cell strain Swan 7I cell survival clinical article coculture enzyme linked immunosorbent assay female fluorescence activated cell sorting human human cell immune response kinetics leukemia leukocyte peripheral blood mononuclear cell regulatory T lymphocyte spontaneous abortion T lymphocyte activation trophoblast Western blotting |
| description |
BACKGROUND: Successful implantation is followed by a local pro-inflammatory and Th1 response, subsequently controlled by Th2. Regulated upon activation, normal T cell expressed and secreted (RANTES) promotes a Th1 response and is implicated as a physiologic tolerogenic factor; therefore, we studied its potential role in the trophoblast-maternal leukocyte dialog. METHODS: We performed co-cultures of immortalized trophoblast cell line (Swan 71) and peripheral blood mononuclear cells (PBMCs) from fertile women (n = 23) or with recurrent spontaneous abortions (n = 18, RSA). After 24 and 48 h, supernatant and cells were analyzed by enzyme-linked immunosorbent assay, fluorescence-activated cell sorting, Western blot and apoptosis assay. To investigate the physiological effects at peripheral level, we co-cultured maternal and paternal PBMCs with conditioned media from Swan cells and progesterone. RESULTS: Following interaction of maternal PBMCs and trophoblast cells, RANTES production increased (P < 0.05) and was accompanied by low levels of interferon γ, interleukin-12 p70 and high levels of tumor necrosis factor-α, nitrites and leukemia-inhibitory factor. RANTES production resulted in elevated apoptosis of potentially deleterious maternal CD3+ lymphocytes, accompanied by a decrease in the proliferative maternal response. During fetal-maternal dialog, the anti-RANTES antibody significantly reduced the frequency of CD4+CD25+Foxp3+ cells (P < 0.05) and was associated with trophoblast cell survival. However, co-cultures of Swan cells and RSA-PBMCs displayed a differential RANTES kinetics, lower levels of regulatory T cells (Tregs) and CD3+annexin-V+cells, accompanied by higher levels of apoptotic trophoblast cells. CONCLUSIONS: RANTES promotes an adequate pro-implantatory microenvironment that influences trophoblast cell survival and modulates the balance of maternal Treg/T effector lymphocytes in favor of maternal tolerance. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. |
| title |
A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation |
| title_short |
A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation |
| title_full |
A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation |
| title_fullStr |
A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation |
| title_full_unstemmed |
A potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced T cell death and regulatory T cell modulation |
| title_sort |
potential tolerogenic immune mechanism in a trophoblast cell line through the activation of chemokine-induced t cell death and regulatory t cell modulation |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v24_n1_p166_Fraccaroli http://hdl.handle.net/20.500.12110/paper_02681161_v24_n1_p166_Fraccaroli |
| _version_ |
1768543178899587072 |