The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo
BACKGROUND: Previous results from our laboratory have led us to propose heparan sulfate (HS) as a putative protamine acceptor during human sperm decondensation in vivo. The aim of this paper was to investigate the presence of glycosaminoglycans in the mammalian oocyte in an effort to better support...
Guardado en:
| Autores principales: | , , |
|---|---|
| Publicado: |
2008
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v23_n5_p1145_Romanato http://hdl.handle.net/20.500.12110/paper_02681161_v23_n5_p1145_Romanato |
| Aporte de: |
| id |
paper:paper_02681161_v23_n5_p1145_Romanato |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_02681161_v23_n5_p1145_Romanato2023-06-08T15:24:09Z The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo Romanato, Marina Julianelli, Vanina Laura Calvo, Juan Carlos Heparan sulfate Oocyte Protamine acceptor Sperm decondensation fluorescent dye glutathione glycosaminoglycan glycosidase heparan sulfate heparin heparin lyase hyaluronidase n acetylgalactosamine 4 sulfatase animal cell animal experiment article confocal microscopy female human immunohistochemistry in vitro study in vivo study male mammal mouse nonhuman normal human oocyte pH measurement semen analysis staining Mammalia BACKGROUND: Previous results from our laboratory have led us to propose heparan sulfate (HS) as a putative protamine acceptor during human sperm decondensation in vivo. The aim of this paper was to investigate the presence of glycosaminoglycans in the mammalian oocyte in an effort to better support this contention. METHODS: Two experimental approaches are used: oocyte labeling to identify the presence of HS and analysis of sperm decondensing ability of fresh oocytes in the presence or absence of specific glycosidases. RESULTS: Staining of mouse zona-intact oocytes with the fluorescent cationic dye, Rubipy, at pH 1.5 allowed for the detection of sulfate residues in the ooplasm by confocal microscopy. HS was detected in the ooplasm by immunocytochemistry. A sperm decondensation microassay using heparin and glutathione was successfully developed. The same level of sperm decondensation could be attained when heparin was replaced by mouse zona-free oocytes. Addition of heparinase to the oocyte/glutathione mixture significantly reduced sperm decondensation (P = 0.0159), while there was no effect following addition of either chondroitinase ABC or hyaluronidase. CONCLUSIONS: The results presented in this paper demonstrate for the first time that HS is present in the mammalian oocyte and show that HS is necessary for fresh oocytes to express their sperm decondensing ability in vitro. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. Fil:Romanato, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Julianelli, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v23_n5_p1145_Romanato http://hdl.handle.net/20.500.12110/paper_02681161_v23_n5_p1145_Romanato |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Heparan sulfate Oocyte Protamine acceptor Sperm decondensation fluorescent dye glutathione glycosaminoglycan glycosidase heparan sulfate heparin heparin lyase hyaluronidase n acetylgalactosamine 4 sulfatase animal cell animal experiment article confocal microscopy female human immunohistochemistry in vitro study in vivo study male mammal mouse nonhuman normal human oocyte pH measurement semen analysis staining Mammalia |
| spellingShingle |
Heparan sulfate Oocyte Protamine acceptor Sperm decondensation fluorescent dye glutathione glycosaminoglycan glycosidase heparan sulfate heparin heparin lyase hyaluronidase n acetylgalactosamine 4 sulfatase animal cell animal experiment article confocal microscopy female human immunohistochemistry in vitro study in vivo study male mammal mouse nonhuman normal human oocyte pH measurement semen analysis staining Mammalia Romanato, Marina Julianelli, Vanina Laura Calvo, Juan Carlos The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo |
| topic_facet |
Heparan sulfate Oocyte Protamine acceptor Sperm decondensation fluorescent dye glutathione glycosaminoglycan glycosidase heparan sulfate heparin heparin lyase hyaluronidase n acetylgalactosamine 4 sulfatase animal cell animal experiment article confocal microscopy female human immunohistochemistry in vitro study in vivo study male mammal mouse nonhuman normal human oocyte pH measurement semen analysis staining Mammalia |
| description |
BACKGROUND: Previous results from our laboratory have led us to propose heparan sulfate (HS) as a putative protamine acceptor during human sperm decondensation in vivo. The aim of this paper was to investigate the presence of glycosaminoglycans in the mammalian oocyte in an effort to better support this contention. METHODS: Two experimental approaches are used: oocyte labeling to identify the presence of HS and analysis of sperm decondensing ability of fresh oocytes in the presence or absence of specific glycosidases. RESULTS: Staining of mouse zona-intact oocytes with the fluorescent cationic dye, Rubipy, at pH 1.5 allowed for the detection of sulfate residues in the ooplasm by confocal microscopy. HS was detected in the ooplasm by immunocytochemistry. A sperm decondensation microassay using heparin and glutathione was successfully developed. The same level of sperm decondensation could be attained when heparin was replaced by mouse zona-free oocytes. Addition of heparinase to the oocyte/glutathione mixture significantly reduced sperm decondensation (P = 0.0159), while there was no effect following addition of either chondroitinase ABC or hyaluronidase. CONCLUSIONS: The results presented in this paper demonstrate for the first time that HS is present in the mammalian oocyte and show that HS is necessary for fresh oocytes to express their sperm decondensing ability in vitro. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. |
| author |
Romanato, Marina Julianelli, Vanina Laura Calvo, Juan Carlos |
| author_facet |
Romanato, Marina Julianelli, Vanina Laura Calvo, Juan Carlos |
| author_sort |
Romanato, Marina |
| title |
The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo |
| title_short |
The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo |
| title_full |
The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo |
| title_fullStr |
The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo |
| title_full_unstemmed |
The presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo |
| title_sort |
presence of heparan sulfate in the mammalian oocyte provides a clue to human sperm nuclear decondensation in vivo |
| publishDate |
2008 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02681161_v23_n5_p1145_Romanato http://hdl.handle.net/20.500.12110/paper_02681161_v23_n5_p1145_Romanato |
| work_keys_str_mv |
AT romanatomarina thepresenceofheparansulfateinthemammalianoocyteprovidesacluetohumanspermnucleardecondensationinvivo AT julianellivaninalaura thepresenceofheparansulfateinthemammalianoocyteprovidesacluetohumanspermnucleardecondensationinvivo AT calvojuancarlos thepresenceofheparansulfateinthemammalianoocyteprovidesacluetohumanspermnucleardecondensationinvivo AT romanatomarina presenceofheparansulfateinthemammalianoocyteprovidesacluetohumanspermnucleardecondensationinvivo AT julianellivaninalaura presenceofheparansulfateinthemammalianoocyteprovidesacluetohumanspermnucleardecondensationinvivo AT calvojuancarlos presenceofheparansulfateinthemammalianoocyteprovidesacluetohumanspermnucleardecondensationinvivo |
| _version_ |
1768546347014684672 |