Involvement of TSSA (trypomastigote small surface antigen) in Trypanosoma cruzi invasion of mammalian cells 1
TSSA (trypomastigote small surface antigen) is a polymorphic mucin-like molecule displayed on the surface of Trypanosoma cruzi trypomastigote forms. To evaluate its functional properties, we undertook comparative biochemical and genetic approaches on isoforms present in parasite stocks from extant e...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v444_n2_p211_Canepa http://hdl.handle.net/20.500.12110/paper_02646021_v444_n2_p211_Canepa |
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paper:paper_02646021_v444_n2_p211_Canepa2023-06-08T15:23:14Z Involvement of TSSA (trypomastigote small surface antigen) in Trypanosoma cruzi invasion of mammalian cells 1 Canepa, Gaspar Exequiel Degese, Maria Sol Buscaglia, Carlos Andrés Calcium Cell invasion Mucin Trypanosoma cruzi Trypomastigote small surface antigen (TSSA) calcium ion complementary DNA membrane antigen mitogen activated protein kinase 1 mitogen activated protein kinase 3 mucin parasite antibody parasite antigen trypomastigote small surface antigen unclassified drug animal cell article binding affinity calcium signaling cell adhesion cell invasion controlled study epimastigote genetic transfection human human cell internalization mammal cell nonhuman nucleotide sequence phagocytosis priority journal protein binding protein expression signal transduction target cell Trypanosoma cruzi trypomastigote Amino Acid Sequence Animals Cercopithecus aethiops HEK293 Cells Humans Molecular Sequence Data Protein Binding Trypanosoma cruzi Variant Surface Glycoproteins, Trypanosoma Vero Cells Mammalia Trypanosoma cruzi TSSA (trypomastigote small surface antigen) is a polymorphic mucin-like molecule displayed on the surface of Trypanosoma cruzi trypomastigote forms. To evaluate its functional properties, we undertook comparative biochemical and genetic approaches on isoforms present in parasite stocks from extant evolutionary lineages (CL Brener and Sylvio X-10). We show that CL Brener TSSA, but not the Sylvio X-10 counterpart, exhibits dosedependent and saturable binding towards non-macrophagic cell lines. This binding triggers Ca 2+ -based signalling responses in the target cell while providing an anchor for the invading parasite.Accordingly, exogenous addition of either TSSA-derived peptides or specific antibodies significantly inhibits invasion of CL Brener, but not Sylvio X-10, trypomastigotes. Non-infective epimastigote forms, which do not express detectable levels of TSSA, were stably transfected with TSSA cDNA from either parasite stock. Although both transfectants produced a surfaceassociated mucin-like TSSA product, epimastigotes expressing CL Brener TSSA showed a ∼2-fold increase in their attachment to mammalian cells. Overall, these findings indicate that CL Brener TSSA functions as a parasite adhesin, engaging surface receptor(s) and inducing signalling pathways on the host cell as a prerequisite for parasite internalization. More importantly, the contrasting functional features of TSSA isoforms provide one appealing mechanism underlying the differential infectivity of T. cruzi stocks. © The Authors Journal compilation © 2012 Biochemical Society. Fil:Cánepa, G.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Degese, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buscaglia, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v444_n2_p211_Canepa http://hdl.handle.net/20.500.12110/paper_02646021_v444_n2_p211_Canepa |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calcium Cell invasion Mucin Trypanosoma cruzi Trypomastigote small surface antigen (TSSA) calcium ion complementary DNA membrane antigen mitogen activated protein kinase 1 mitogen activated protein kinase 3 mucin parasite antibody parasite antigen trypomastigote small surface antigen unclassified drug animal cell article binding affinity calcium signaling cell adhesion cell invasion controlled study epimastigote genetic transfection human human cell internalization mammal cell nonhuman nucleotide sequence phagocytosis priority journal protein binding protein expression signal transduction target cell Trypanosoma cruzi trypomastigote Amino Acid Sequence Animals Cercopithecus aethiops HEK293 Cells Humans Molecular Sequence Data Protein Binding Trypanosoma cruzi Variant Surface Glycoproteins, Trypanosoma Vero Cells Mammalia Trypanosoma cruzi |
| spellingShingle |
Calcium Cell invasion Mucin Trypanosoma cruzi Trypomastigote small surface antigen (TSSA) calcium ion complementary DNA membrane antigen mitogen activated protein kinase 1 mitogen activated protein kinase 3 mucin parasite antibody parasite antigen trypomastigote small surface antigen unclassified drug animal cell article binding affinity calcium signaling cell adhesion cell invasion controlled study epimastigote genetic transfection human human cell internalization mammal cell nonhuman nucleotide sequence phagocytosis priority journal protein binding protein expression signal transduction target cell Trypanosoma cruzi trypomastigote Amino Acid Sequence Animals Cercopithecus aethiops HEK293 Cells Humans Molecular Sequence Data Protein Binding Trypanosoma cruzi Variant Surface Glycoproteins, Trypanosoma Vero Cells Mammalia Trypanosoma cruzi Canepa, Gaspar Exequiel Degese, Maria Sol Buscaglia, Carlos Andrés Involvement of TSSA (trypomastigote small surface antigen) in Trypanosoma cruzi invasion of mammalian cells 1 |
| topic_facet |
Calcium Cell invasion Mucin Trypanosoma cruzi Trypomastigote small surface antigen (TSSA) calcium ion complementary DNA membrane antigen mitogen activated protein kinase 1 mitogen activated protein kinase 3 mucin parasite antibody parasite antigen trypomastigote small surface antigen unclassified drug animal cell article binding affinity calcium signaling cell adhesion cell invasion controlled study epimastigote genetic transfection human human cell internalization mammal cell nonhuman nucleotide sequence phagocytosis priority journal protein binding protein expression signal transduction target cell Trypanosoma cruzi trypomastigote Amino Acid Sequence Animals Cercopithecus aethiops HEK293 Cells Humans Molecular Sequence Data Protein Binding Trypanosoma cruzi Variant Surface Glycoproteins, Trypanosoma Vero Cells Mammalia Trypanosoma cruzi |
| description |
TSSA (trypomastigote small surface antigen) is a polymorphic mucin-like molecule displayed on the surface of Trypanosoma cruzi trypomastigote forms. To evaluate its functional properties, we undertook comparative biochemical and genetic approaches on isoforms present in parasite stocks from extant evolutionary lineages (CL Brener and Sylvio X-10). We show that CL Brener TSSA, but not the Sylvio X-10 counterpart, exhibits dosedependent and saturable binding towards non-macrophagic cell lines. This binding triggers Ca 2+ -based signalling responses in the target cell while providing an anchor for the invading parasite.Accordingly, exogenous addition of either TSSA-derived peptides or specific antibodies significantly inhibits invasion of CL Brener, but not Sylvio X-10, trypomastigotes. Non-infective epimastigote forms, which do not express detectable levels of TSSA, were stably transfected with TSSA cDNA from either parasite stock. Although both transfectants produced a surfaceassociated mucin-like TSSA product, epimastigotes expressing CL Brener TSSA showed a ∼2-fold increase in their attachment to mammalian cells. Overall, these findings indicate that CL Brener TSSA functions as a parasite adhesin, engaging surface receptor(s) and inducing signalling pathways on the host cell as a prerequisite for parasite internalization. More importantly, the contrasting functional features of TSSA isoforms provide one appealing mechanism underlying the differential infectivity of T. cruzi stocks. © The Authors Journal compilation © 2012 Biochemical Society. |
| author |
Canepa, Gaspar Exequiel Degese, Maria Sol Buscaglia, Carlos Andrés |
| author_facet |
Canepa, Gaspar Exequiel Degese, Maria Sol Buscaglia, Carlos Andrés |
| author_sort |
Canepa, Gaspar Exequiel |
| title |
Involvement of TSSA (trypomastigote small surface antigen) in Trypanosoma cruzi invasion of mammalian cells 1 |
| title_short |
Involvement of TSSA (trypomastigote small surface antigen) in Trypanosoma cruzi invasion of mammalian cells 1 |
| title_full |
Involvement of TSSA (trypomastigote small surface antigen) in Trypanosoma cruzi invasion of mammalian cells 1 |
| title_fullStr |
Involvement of TSSA (trypomastigote small surface antigen) in Trypanosoma cruzi invasion of mammalian cells 1 |
| title_full_unstemmed |
Involvement of TSSA (trypomastigote small surface antigen) in Trypanosoma cruzi invasion of mammalian cells 1 |
| title_sort |
involvement of tssa (trypomastigote small surface antigen) in trypanosoma cruzi invasion of mammalian cells 1 |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v444_n2_p211_Canepa http://hdl.handle.net/20.500.12110/paper_02646021_v444_n2_p211_Canepa |
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