Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells
We examined the role of protein kinase Cα (PKCα) in the stimulation of DNA synthesis of Swiss 3T3 cells induced by bombesin, platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA). We found that cells in which this kinase had been down-regulated showed a partially abrogated...
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1996
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paper:paper_02646021_v315_n2_p513_FlorinChristensen2023-06-08T15:23:09Z Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells Florin-Christensen, Jorge Jacobsen de Florín-Christensen, Mónica O. Meinardi, Elsa Noemí 2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia We examined the role of protein kinase Cα (PKCα) in the stimulation of DNA synthesis of Swiss 3T3 cells induced by bombesin, platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA). We found that cells in which this kinase had been down-regulated showed a partially abrogated mitogenic response to bombesin. The response to PDGF was unaltered; however, the response to PMA was completely suppressed. The mitogenic effect of maximal doses of bombesin and PMA combined was greater than that of either agent alone, suggesting that bombesin does not fully activate the PKC pathway. Accordingly, bombesin-induced PKCα translocation from cytosol to membranes was partial, while that observed with PMA was essentially complete. Moreover, exposure to Ro-31-8220, a PKC inhibitor, had signficantly greater effects on the response to PMA than on that to bombesin. Our findings point out different roles that PKCα may play in diversely activated cells: while, in the case of PMA, stimulation of this kinase may be necessary and sufficient to induce proliferation, it appears to be necessary only for a full response to bombesin, and redundant among the mechanisms triggered by PDGF. Fil:Florin-Christensen, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Florin-Christensen, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Meinardi, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v315_n2_p513_FlorinChristensen http://hdl.handle.net/20.500.12110/paper_02646021_v315_n2_p513_FlorinChristensen |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia |
| spellingShingle |
2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia Florin-Christensen, Jorge Jacobsen de Florín-Christensen, Mónica O. Meinardi, Elsa Noemí Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells |
| topic_facet |
2 [1 (3 amidinothiopropyl) 1h indol 3 yl] 3 (1 methyl 1h indol 3 yl)maleimide bombesin DNA phorbol 13 acetate 12 myristate platelet derived growth factor protein kinase C protein kinase C inhibitor animal cell article cell proliferation cell strain 3T3 controlled study DNA synthesis enzyme activation enzyme subunit mitogenesis mouse nonhuman priority journal protein transport Animalia |
| description |
We examined the role of protein kinase Cα (PKCα) in the stimulation of DNA synthesis of Swiss 3T3 cells induced by bombesin, platelet-derived growth factor (PDGF) and phorbol 12-myristate 13-acetate (PMA). We found that cells in which this kinase had been down-regulated showed a partially abrogated mitogenic response to bombesin. The response to PDGF was unaltered; however, the response to PMA was completely suppressed. The mitogenic effect of maximal doses of bombesin and PMA combined was greater than that of either agent alone, suggesting that bombesin does not fully activate the PKC pathway. Accordingly, bombesin-induced PKCα translocation from cytosol to membranes was partial, while that observed with PMA was essentially complete. Moreover, exposure to Ro-31-8220, a PKC inhibitor, had signficantly greater effects on the response to PMA than on that to bombesin. Our findings point out different roles that PKCα may play in diversely activated cells: while, in the case of PMA, stimulation of this kinase may be necessary and sufficient to induce proliferation, it appears to be necessary only for a full response to bombesin, and redundant among the mechanisms triggered by PDGF. |
| author |
Florin-Christensen, Jorge Jacobsen de Florín-Christensen, Mónica O. Meinardi, Elsa Noemí |
| author_facet |
Florin-Christensen, Jorge Jacobsen de Florín-Christensen, Mónica O. Meinardi, Elsa Noemí |
| author_sort |
Florin-Christensen, Jorge |
| title |
Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells |
| title_short |
Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells |
| title_full |
Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells |
| title_fullStr |
Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells |
| title_full_unstemmed |
Diversity of roles of protein kinase Cα in the proliferation of Swiss 3T3 cells |
| title_sort |
diversity of roles of protein kinase cα in the proliferation of swiss 3t3 cells |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v315_n2_p513_FlorinChristensen http://hdl.handle.net/20.500.12110/paper_02646021_v315_n2_p513_FlorinChristensen |
| work_keys_str_mv |
AT florinchristensenjorge diversityofrolesofproteinkinasecaintheproliferationofswiss3t3cells AT jacobsendeflorinchristensenmonicao diversityofrolesofproteinkinasecaintheproliferationofswiss3t3cells AT meinardielsanoemi diversityofrolesofproteinkinasecaintheproliferationofswiss3t3cells |
| _version_ |
1768543656274296832 |