A DNA topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone
Follicle-stimulating hormone (FSH), acting through a cyclic AMP-mediated mechanism, promotes differentiation of rat granulosa cells cultured in a defined medium. Camptothecin, a DNA topoisomerase I blocker, inhibited the increase in progesterone and oestradiol production stimulated by FSH. This effe...
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1991
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v277_n2_p557_Baranao http://hdl.handle.net/20.500.12110/paper_02646021_v277_n2_p557_Baranao |
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paper:paper_02646021_v277_n2_p557_Baranao |
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paper:paper_02646021_v277_n2_p557_Baranao2023-06-08T15:23:07Z A DNA topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone bucladesine camptothecin cell extract DNA topoisomerase estradiol follitropin progesterone bucladesine camptothecin diethylstilbestrol DNA topoisomerase follitropin insulin methionine camptothecin DNA topoisomerase estradiol progesterone animal cell animal cell culture Article cell differentiation comparative study controlled study enzyme activity enzyme assay female granulosa cell hormone synthesis nonhuman priority journal protein synthesis rat animal article cell culture cytology drug antagonism drug effect enzymology kinetics metabolism rat strain DNA synthesis gene expression regulation hormonal regulation topical drug administration Animals Bucladesine Camptothecin Cell Differentiation Cells, Cultured Diethylstilbestrol DNA Topoisomerases, Type I Female Follicle Stimulating Hormone Granulosa Cells Insulin Kinetics Methionine Protein Biosynthesis Rats Rats, Inbred Strains Follicle-stimulating hormone (FSH), acting through a cyclic AMP-mediated mechanism, promotes differentiation of rat granulosa cells cultured in a defined medium. Camptothecin, a DNA topoisomerase I blocker, inhibited the increase in progesterone and oestradiol production stimulated by FSH. This effect was not due to non-specific inhibition of protein synthesis, as shown by measurement of [35S]methionine incorporation. A transient increase in DNA topoisomerase I activity was observed after 24 h of culture in the presence of FSH or dibutyryl cyclic AMP. Our results are consistent with a key role for DNA topoisomerase I in the modulation of gene expression by FSH in rat granulosa cells. © Portland Press Ltd. All rights reserved. 1991 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v277_n2_p557_Baranao http://hdl.handle.net/20.500.12110/paper_02646021_v277_n2_p557_Baranao |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bucladesine camptothecin cell extract DNA topoisomerase estradiol follitropin progesterone bucladesine camptothecin diethylstilbestrol DNA topoisomerase follitropin insulin methionine camptothecin DNA topoisomerase estradiol progesterone animal cell animal cell culture Article cell differentiation comparative study controlled study enzyme activity enzyme assay female granulosa cell hormone synthesis nonhuman priority journal protein synthesis rat animal article cell culture cytology drug antagonism drug effect enzymology kinetics metabolism rat strain DNA synthesis gene expression regulation hormonal regulation topical drug administration Animals Bucladesine Camptothecin Cell Differentiation Cells, Cultured Diethylstilbestrol DNA Topoisomerases, Type I Female Follicle Stimulating Hormone Granulosa Cells Insulin Kinetics Methionine Protein Biosynthesis Rats Rats, Inbred Strains |
| spellingShingle |
bucladesine camptothecin cell extract DNA topoisomerase estradiol follitropin progesterone bucladesine camptothecin diethylstilbestrol DNA topoisomerase follitropin insulin methionine camptothecin DNA topoisomerase estradiol progesterone animal cell animal cell culture Article cell differentiation comparative study controlled study enzyme activity enzyme assay female granulosa cell hormone synthesis nonhuman priority journal protein synthesis rat animal article cell culture cytology drug antagonism drug effect enzymology kinetics metabolism rat strain DNA synthesis gene expression regulation hormonal regulation topical drug administration Animals Bucladesine Camptothecin Cell Differentiation Cells, Cultured Diethylstilbestrol DNA Topoisomerases, Type I Female Follicle Stimulating Hormone Granulosa Cells Insulin Kinetics Methionine Protein Biosynthesis Rats Rats, Inbred Strains A DNA topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone |
| topic_facet |
bucladesine camptothecin cell extract DNA topoisomerase estradiol follitropin progesterone bucladesine camptothecin diethylstilbestrol DNA topoisomerase follitropin insulin methionine camptothecin DNA topoisomerase estradiol progesterone animal cell animal cell culture Article cell differentiation comparative study controlled study enzyme activity enzyme assay female granulosa cell hormone synthesis nonhuman priority journal protein synthesis rat animal article cell culture cytology drug antagonism drug effect enzymology kinetics metabolism rat strain DNA synthesis gene expression regulation hormonal regulation topical drug administration Animals Bucladesine Camptothecin Cell Differentiation Cells, Cultured Diethylstilbestrol DNA Topoisomerases, Type I Female Follicle Stimulating Hormone Granulosa Cells Insulin Kinetics Methionine Protein Biosynthesis Rats Rats, Inbred Strains |
| description |
Follicle-stimulating hormone (FSH), acting through a cyclic AMP-mediated mechanism, promotes differentiation of rat granulosa cells cultured in a defined medium. Camptothecin, a DNA topoisomerase I blocker, inhibited the increase in progesterone and oestradiol production stimulated by FSH. This effect was not due to non-specific inhibition of protein synthesis, as shown by measurement of [35S]methionine incorporation. A transient increase in DNA topoisomerase I activity was observed after 24 h of culture in the presence of FSH or dibutyryl cyclic AMP. Our results are consistent with a key role for DNA topoisomerase I in the modulation of gene expression by FSH in rat granulosa cells. © Portland Press Ltd. All rights reserved. |
| title |
A DNA topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone |
| title_short |
A DNA topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone |
| title_full |
A DNA topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone |
| title_fullStr |
A DNA topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone |
| title_full_unstemmed |
A DNA topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone |
| title_sort |
dna topoisomerase i inhibitor blocks the differentiation of rat granulosa cells induced by follicle-stimulating hormone |
| publishDate |
1991 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v277_n2_p557_Baranao http://hdl.handle.net/20.500.12110/paper_02646021_v277_n2_p557_Baranao |
| _version_ |
1768546346628808704 |