Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa).
An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cati...
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1988
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v249_n3_p807_Carricarte http://hdl.handle.net/20.500.12110/paper_02646021_v249_n3_p807_Carricarte |
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paper:paper_02646021_v249_n3_p807_Carricarte2023-06-08T15:23:07Z Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). Carricarte, Valentina Bianchini, Graciela Mabel Muschietti, Jorge P. Torres, Héctor Norberto Flawiá, Mirtha María adenylate cyclase calcium calmodulin chlorpromazine egtazic acid fluphenazine alfalfa article density gradient centrifugation drug antagonism enzymology gel chromatography ion exchange chromatography metabolism Adenylate Cyclase Calcium Calmodulin Centrifugation, Density Gradient Chlorpromazine Chromatography, DEAE-Cellulose Chromatography, Gel Egtazic Acid Fluphenazine Medicago sativa An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase. Fil:Carricarte, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bianchini, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v249_n3_p807_Carricarte http://hdl.handle.net/20.500.12110/paper_02646021_v249_n3_p807_Carricarte |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenylate cyclase calcium calmodulin chlorpromazine egtazic acid fluphenazine alfalfa article density gradient centrifugation drug antagonism enzymology gel chromatography ion exchange chromatography metabolism Adenylate Cyclase Calcium Calmodulin Centrifugation, Density Gradient Chlorpromazine Chromatography, DEAE-Cellulose Chromatography, Gel Egtazic Acid Fluphenazine Medicago sativa |
| spellingShingle |
adenylate cyclase calcium calmodulin chlorpromazine egtazic acid fluphenazine alfalfa article density gradient centrifugation drug antagonism enzymology gel chromatography ion exchange chromatography metabolism Adenylate Cyclase Calcium Calmodulin Centrifugation, Density Gradient Chlorpromazine Chromatography, DEAE-Cellulose Chromatography, Gel Egtazic Acid Fluphenazine Medicago sativa Carricarte, Valentina Bianchini, Graciela Mabel Muschietti, Jorge P. Torres, Héctor Norberto Flawiá, Mirtha María Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). |
| topic_facet |
adenylate cyclase calcium calmodulin chlorpromazine egtazic acid fluphenazine alfalfa article density gradient centrifugation drug antagonism enzymology gel chromatography ion exchange chromatography metabolism Adenylate Cyclase Calcium Calmodulin Centrifugation, Density Gradient Chlorpromazine Chromatography, DEAE-Cellulose Chromatography, Gel Egtazic Acid Fluphenazine Medicago sativa |
| description |
An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leaves activates this adenylate cyclase. |
| author |
Carricarte, Valentina Bianchini, Graciela Mabel Muschietti, Jorge P. Torres, Héctor Norberto Flawiá, Mirtha María |
| author_facet |
Carricarte, Valentina Bianchini, Graciela Mabel Muschietti, Jorge P. Torres, Héctor Norberto Flawiá, Mirtha María |
| author_sort |
Carricarte, Valentina |
| title |
Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). |
| title_short |
Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). |
| title_full |
Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). |
| title_fullStr |
Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). |
| title_full_unstemmed |
Adenylate cyclase activity in a higher plant, alfalfa (Medicago sativa). |
| title_sort |
adenylate cyclase activity in a higher plant, alfalfa (medicago sativa). |
| publishDate |
1988 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02646021_v249_n3_p807_Carricarte http://hdl.handle.net/20.500.12110/paper_02646021_v249_n3_p807_Carricarte |
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| _version_ |
1768546157457309696 |