Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers

Bovine mastitis produces economic losses, attributable to the decrease in milk production, reduced milk quality, costs of treatment and replacement of animals. A successful prophylactic vaccine against Staphylococcus aureus should elicit both humoral and cellular immune responses. In a previous repo...

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Detalles Bibliográficos
Publicado:	2016
Materias:	Avirulent mutant Bovine mastitis Staphylococcus aureus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0264410X_v34_n29_p3356_Pellegrino http://hdl.handle.net/20.500.12110/paper_0264410X_v34_n29_p3356_Pellegrino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0264410X_v34_n29_p3356_Pellegrino
record_format	dspace
spelling	paper:paper_0264410X_v34_n29_p3356_Pellegrino2023-06-08T15:23:04Z Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers Avirulent mutant Bovine mastitis Staphylococcus aureus Bovine mastitis produces economic losses, attributable to the decrease in milk production, reduced milk quality, costs of treatment and replacement of animals. A successful prophylactic vaccine against Staphylococcus aureus should elicit both humoral and cellular immune responses. In a previous report we evaluated the effectiveness of a live vaccine to protect heifers against challenge with a virulent strain. In the present study the immunological response of heifers after combined immunization schedule was investigated. In a first experimental trial, heifers were vaccinated with 3 subcutaneous doses of avirulent mutant S. aureus RC122 before calving and one intramammary dose (IMD) after calving. Antibodies concentration in blood, bactericidal effect of serum from vaccinated animals and lymphocyte proliferation was determined. The levels of total IgG, IgG1 and IgG2 in colostrum and the lymphocyte proliferation index were significantly higher in vaccinated respect to non-vaccinated group throughout the experiment. The second trial, where animals were inoculated with different vaccination schedules, was carried out to determine the effect of the IMD on the level of antibodies in blood and milk, cytokines (IL-13 and IFN-γ) concentration and milk's SCC and bacteriology. The bacterial growth of the S. aureus strains was totally inhibited at 1–3 × 106 and 1–3 × 103 cfu/ml, when the strains were mixed with pooled serum diluted 1/40. The results shown that IMD has not a significant effect on the features determinate. In conclusion, a vaccination schedule involving three SC doses before calving would be enough to stimulate antibodies production in milk without an IMD. Furthermore, the results showed a bactericidal effect of serum from vaccinated animals and this provides further evidence about serum functionality. Immune responses, humoral (antigen-specific antibodies and Th2 type cytokines) and cellular (T-lymphocyte proliferation responses and Th1 type cytokines), were augmented by administration of the avirulent mutant which represent an antigenic pool. © 2016 Elsevier Ltd 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0264410X_v34_n29_p3356_Pellegrino http://hdl.handle.net/20.500.12110/paper_0264410X_v34_n29_p3356_Pellegrino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Avirulent mutant Bovine mastitis Staphylococcus aureus
spellingShingle	Avirulent mutant Bovine mastitis Staphylococcus aureus Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers
topic_facet	Avirulent mutant Bovine mastitis Staphylococcus aureus
description	Bovine mastitis produces economic losses, attributable to the decrease in milk production, reduced milk quality, costs of treatment and replacement of animals. A successful prophylactic vaccine against Staphylococcus aureus should elicit both humoral and cellular immune responses. In a previous report we evaluated the effectiveness of a live vaccine to protect heifers against challenge with a virulent strain. In the present study the immunological response of heifers after combined immunization schedule was investigated. In a first experimental trial, heifers were vaccinated with 3 subcutaneous doses of avirulent mutant S. aureus RC122 before calving and one intramammary dose (IMD) after calving. Antibodies concentration in blood, bactericidal effect of serum from vaccinated animals and lymphocyte proliferation was determined. The levels of total IgG, IgG1 and IgG2 in colostrum and the lymphocyte proliferation index were significantly higher in vaccinated respect to non-vaccinated group throughout the experiment. The second trial, where animals were inoculated with different vaccination schedules, was carried out to determine the effect of the IMD on the level of antibodies in blood and milk, cytokines (IL-13 and IFN-γ) concentration and milk's SCC and bacteriology. The bacterial growth of the S. aureus strains was totally inhibited at 1–3 × 106 and 1–3 × 103 cfu/ml, when the strains were mixed with pooled serum diluted 1/40. The results shown that IMD has not a significant effect on the features determinate. In conclusion, a vaccination schedule involving three SC doses before calving would be enough to stimulate antibodies production in milk without an IMD. Furthermore, the results showed a bactericidal effect of serum from vaccinated animals and this provides further evidence about serum functionality. Immune responses, humoral (antigen-specific antibodies and Th2 type cytokines) and cellular (T-lymphocyte proliferation responses and Th1 type cytokines), were augmented by administration of the avirulent mutant which represent an antigenic pool. © 2016 Elsevier Ltd
title	Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers
title_short	Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers
title_full	Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers
title_fullStr	Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers
title_full_unstemmed	Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers
title_sort	staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0264410X_v34_n29_p3356_Pellegrino http://hdl.handle.net/20.500.12110/paper_0264410X_v34_n29_p3356_Pellegrino
_version_	1768543081865412608

Staphylococcus aureus avirulent mutant vaccine induces humoral and cellular immune responses on pregnant heifers

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