Changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases

Bone metastasis is an incurable complication of breast cancer affecting 70-80 % of advanced patients. It is a multistep process that includes tumour cell mobilisation, intravasation, survival in the circulation, extravasation, migration and proliferation in the bone marrow/bone. Although novel findi...

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Detalles Bibliográficos
Publicado: 2014
Materias:
Bone marrow
Bone metastasis
Breast cancer
Mesenchymal stem cells
Pre-metastatic niche
Apoptosis
Bone Marrow
Bone Neoplasms
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Neoplasm Metastasis
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02620898_v31_n2_p213_Martinez
http://hdl.handle.net/20.500.12110/paper_02620898_v31_n2_p213_Martinez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_02620898_v31_n2_p213_Martinez
record_format dspace
spelling paper:paper_02620898_v31_n2_p213_Martinez2023-06-08T15:22:49Z Changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases Bone marrow Bone metastasis Breast cancer Mesenchymal stem cells Pre-metastatic niche Apoptosis Bone Marrow Bone Neoplasms Breast Neoplasms Cell Line, Tumor Cell Proliferation Enzyme-Linked Immunosorbent Assay Female Humans Immunohistochemistry Neoplasm Metastasis Bone metastasis is an incurable complication of breast cancer affecting 70-80 % of advanced patients. It is a multistep process that includes tumour cell mobilisation, intravasation, survival in the circulation, extravasation, migration and proliferation in the bone marrow/bone. Although novel findings demonstrate the bone marrow microenvironment significance in bone metastatic progression, a majority of studies have focused on end-stage disease and little is known about how the pre-metastatic niche arises in the bone marrow/bone tissues. We demonstrated a significant increase in patients' peripheral blood plasma ability to induce transendothelial migration of MCF-7 cells compared with healthy volunteers. Moreover, high RANKL, MIF and OPG levels in patients' peripheral blood could play a role in the intravasation, angiogenesis, survival and epithelial-mesenchymal transition of circulating tumour cells. Also, we observed a significant increase in patients' bone marrow plasma capacity to induce transendothelial migration of MDA-MB231 and MCF-7 cells compared with healthy volunteers. Furthermore, patients' bone marrow mesenchymal stem cells could control the recruitment of tumour cells, modifying the MCF-7 and MDA-MB231 cell migration. In addition, we found a significantly higher MDA-MB231 cell proliferation when we used patients' bone marrow plasma compared with healthy volunteers. Interestingly, PDGF-AB, ICAM-1 and VCAM-1 levels in patients' bone marrow were significantly higher than the values of healthy volunteers, suggesting that they could be involved in the cancer cell extravasation, bone resorption and cancer cell proliferation. We believe that these results can reveal new information about what alterations happen in the bone marrow of advanced breast cancer patients before bone colonisation, changes that create optimal soil for the metastatic cascade progression. © 2013 Springer Science+Business Media Dordrecht. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02620898_v31_n2_p213_Martinez http://hdl.handle.net/20.500.12110/paper_02620898_v31_n2_p213_Martinez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bone marrow
Bone metastasis
Breast cancer
Mesenchymal stem cells
Pre-metastatic niche
Apoptosis
Bone Marrow
Bone Neoplasms
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Neoplasm Metastasis
spellingShingle Bone marrow
Bone metastasis
Breast cancer
Mesenchymal stem cells
Pre-metastatic niche
Apoptosis
Bone Marrow
Bone Neoplasms
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Neoplasm Metastasis
Changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases
topic_facet Bone marrow
Bone metastasis
Breast cancer
Mesenchymal stem cells
Pre-metastatic niche
Apoptosis
Bone Marrow
Bone Neoplasms
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Neoplasm Metastasis
description Bone metastasis is an incurable complication of breast cancer affecting 70-80 % of advanced patients. It is a multistep process that includes tumour cell mobilisation, intravasation, survival in the circulation, extravasation, migration and proliferation in the bone marrow/bone. Although novel findings demonstrate the bone marrow microenvironment significance in bone metastatic progression, a majority of studies have focused on end-stage disease and little is known about how the pre-metastatic niche arises in the bone marrow/bone tissues. We demonstrated a significant increase in patients' peripheral blood plasma ability to induce transendothelial migration of MCF-7 cells compared with healthy volunteers. Moreover, high RANKL, MIF and OPG levels in patients' peripheral blood could play a role in the intravasation, angiogenesis, survival and epithelial-mesenchymal transition of circulating tumour cells. Also, we observed a significant increase in patients' bone marrow plasma capacity to induce transendothelial migration of MDA-MB231 and MCF-7 cells compared with healthy volunteers. Furthermore, patients' bone marrow mesenchymal stem cells could control the recruitment of tumour cells, modifying the MCF-7 and MDA-MB231 cell migration. In addition, we found a significantly higher MDA-MB231 cell proliferation when we used patients' bone marrow plasma compared with healthy volunteers. Interestingly, PDGF-AB, ICAM-1 and VCAM-1 levels in patients' bone marrow were significantly higher than the values of healthy volunteers, suggesting that they could be involved in the cancer cell extravasation, bone resorption and cancer cell proliferation. We believe that these results can reveal new information about what alterations happen in the bone marrow of advanced breast cancer patients before bone colonisation, changes that create optimal soil for the metastatic cascade progression. © 2013 Springer Science+Business Media Dordrecht.
title Changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases
title_short Changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases
title_full Changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases
title_fullStr Changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases
title_full_unstemmed Changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases
title_sort changes in the peripheral blood and bone marrow from untreated advanced breast cancer patients that are associated with the establishment of bone metastases
publishDate 2014
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02620898_v31_n2_p213_Martinez
http://hdl.handle.net/20.500.12110/paper_02620898_v31_n2_p213_Martinez
_version_ 1768545372710371328

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