Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing
Here we investigate the promoter control of alternative splicing by studying two transcriptional activators on templates under replicating conditions. SV40 large T-antigen (T-Ag) activates template replication only 2-fold but transcription 25-fold. T-Ag-mediated replication, reported to inhibit RNA...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02614189_v20_n20_p5759_Kadener http://hdl.handle.net/20.500.12110/paper_02614189_v20_n20_p5759_Kadener |
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paper:paper_02614189_v20_n20_p5759_Kadener2023-06-08T15:22:45Z Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing Alternative splicing SV40 T-Ag VP16 etoposide fibronectin RNA polymerase II virus large T antigen alternative RNA splicing animal cell article cell transformation controlled study DNA replication DNA template exon human human cell Human immunodeficiency virus nonhuman priority journal promoter region Simian virus 40 structural gene transcription initiation transcription regulation Alternative Splicing Animals Antigens, Polyomavirus Transforming Carcinoma, Hepatocellular Cercopithecus aethiops COS Cells DNA Replication DNA, Recombinant Exons Fibronectins Herpes Simplex Virus Protein Vmw65 Humans In Situ Hybridization Liver Neoplasms Promoter Regions (Genetics) Recombinant Fusion Proteins Reverse Transcriptase Polymerase Chain Reaction RNA Polymerase II RNA, Messenger Simian virus 40 Templates, Genetic Transcription, Genetic Transfection Animalia DNA viruses Human immunodeficiency virus RNA viruses Simiae Simian virus Simian virus 40 Here we investigate the promoter control of alternative splicing by studying two transcriptional activators on templates under replicating conditions. SV40 large T-antigen (T-Ag) activates template replication only 2-fold but transcription 25-fold. T-Ag-mediated replication, reported to inhibit RNA polymerase II elongation, provokes a 10- to 30-fold increase in the inclusion of the fibronectin EDI exon into mature mRNA. The T-Ag effect is exon specific, occurs in cis and depends strictly on DNA replication and not on cell transformation. VP16, an activator of transcriptional initiation and elongation, has a similar effect on transcription but the opposite effect on splicing: EDI inclusion is inhibited by 35-fold. VP16 completely reverts the T-Ag effect, but a VP16 mutant with reduced elongation ability provokes only partial reversion. Both T-Ag and VP16 promote conspicuous co-localization of mRNA with nuclear speckles that contain the SR protein SF2/ASF, a positive regulator of EDI inclusion. Therefore, we conclude that co-localization of transcripts and speckles is not sufficient to stimulate EDI inclusion. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02614189_v20_n20_p5759_Kadener http://hdl.handle.net/20.500.12110/paper_02614189_v20_n20_p5759_Kadener |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Alternative splicing SV40 T-Ag VP16 etoposide fibronectin RNA polymerase II virus large T antigen alternative RNA splicing animal cell article cell transformation controlled study DNA replication DNA template exon human human cell Human immunodeficiency virus nonhuman priority journal promoter region Simian virus 40 structural gene transcription initiation transcription regulation Alternative Splicing Animals Antigens, Polyomavirus Transforming Carcinoma, Hepatocellular Cercopithecus aethiops COS Cells DNA Replication DNA, Recombinant Exons Fibronectins Herpes Simplex Virus Protein Vmw65 Humans In Situ Hybridization Liver Neoplasms Promoter Regions (Genetics) Recombinant Fusion Proteins Reverse Transcriptase Polymerase Chain Reaction RNA Polymerase II RNA, Messenger Simian virus 40 Templates, Genetic Transcription, Genetic Transfection Animalia DNA viruses Human immunodeficiency virus RNA viruses Simiae Simian virus Simian virus 40 |
| spellingShingle |
Alternative splicing SV40 T-Ag VP16 etoposide fibronectin RNA polymerase II virus large T antigen alternative RNA splicing animal cell article cell transformation controlled study DNA replication DNA template exon human human cell Human immunodeficiency virus nonhuman priority journal promoter region Simian virus 40 structural gene transcription initiation transcription regulation Alternative Splicing Animals Antigens, Polyomavirus Transforming Carcinoma, Hepatocellular Cercopithecus aethiops COS Cells DNA Replication DNA, Recombinant Exons Fibronectins Herpes Simplex Virus Protein Vmw65 Humans In Situ Hybridization Liver Neoplasms Promoter Regions (Genetics) Recombinant Fusion Proteins Reverse Transcriptase Polymerase Chain Reaction RNA Polymerase II RNA, Messenger Simian virus 40 Templates, Genetic Transcription, Genetic Transfection Animalia DNA viruses Human immunodeficiency virus RNA viruses Simiae Simian virus Simian virus 40 Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing |
| topic_facet |
Alternative splicing SV40 T-Ag VP16 etoposide fibronectin RNA polymerase II virus large T antigen alternative RNA splicing animal cell article cell transformation controlled study DNA replication DNA template exon human human cell Human immunodeficiency virus nonhuman priority journal promoter region Simian virus 40 structural gene transcription initiation transcription regulation Alternative Splicing Animals Antigens, Polyomavirus Transforming Carcinoma, Hepatocellular Cercopithecus aethiops COS Cells DNA Replication DNA, Recombinant Exons Fibronectins Herpes Simplex Virus Protein Vmw65 Humans In Situ Hybridization Liver Neoplasms Promoter Regions (Genetics) Recombinant Fusion Proteins Reverse Transcriptase Polymerase Chain Reaction RNA Polymerase II RNA, Messenger Simian virus 40 Templates, Genetic Transcription, Genetic Transfection Animalia DNA viruses Human immunodeficiency virus RNA viruses Simiae Simian virus Simian virus 40 |
| description |
Here we investigate the promoter control of alternative splicing by studying two transcriptional activators on templates under replicating conditions. SV40 large T-antigen (T-Ag) activates template replication only 2-fold but transcription 25-fold. T-Ag-mediated replication, reported to inhibit RNA polymerase II elongation, provokes a 10- to 30-fold increase in the inclusion of the fibronectin EDI exon into mature mRNA. The T-Ag effect is exon specific, occurs in cis and depends strictly on DNA replication and not on cell transformation. VP16, an activator of transcriptional initiation and elongation, has a similar effect on transcription but the opposite effect on splicing: EDI inclusion is inhibited by 35-fold. VP16 completely reverts the T-Ag effect, but a VP16 mutant with reduced elongation ability provokes only partial reversion. Both T-Ag and VP16 promote conspicuous co-localization of mRNA with nuclear speckles that contain the SR protein SF2/ASF, a positive regulator of EDI inclusion. Therefore, we conclude that co-localization of transcripts and speckles is not sufficient to stimulate EDI inclusion. |
| title |
Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing |
| title_short |
Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing |
| title_full |
Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing |
| title_fullStr |
Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing |
| title_full_unstemmed |
Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing |
| title_sort |
antagonistic effects of t-ag and vp16 reveal a role for rna pol ii elongation on alternative splicing |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02614189_v20_n20_p5759_Kadener http://hdl.handle.net/20.500.12110/paper_02614189_v20_n20_p5759_Kadener |
| _version_ |
1768543132281995264 |