Lignocellulolytic enzymes from Fomes sclerodermeus growing in solid-state fermentation

To investigate the growth and production of lignocellulosic enzymes from Fomes sclerodermeus, solid-state fermentation (SSF) was performed by using soy and wheat bran. Both media showed similar maximal values of dry weight loss of the substrate and biomass production, nevertheless enzyme production...

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Autores principales: Papinutti, Víctor Leandro, Forchíassin, Flavia
Publicado: 2007
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v81_n1_p54_Papinutti
http://hdl.handle.net/20.500.12110/paper_02608774_v81_n1_p54_Papinutti
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spelling paper:paper_02608774_v81_n1_p54_Papinutti2023-06-08T15:22:38Z Lignocellulolytic enzymes from Fomes sclerodermeus growing in solid-state fermentation Papinutti, Víctor Leandro Forchíassin, Flavia Endoglucanase Endoxylanase Fomes sclerodermeus Laccase Manganese peroxidase Polygalacturonase Biomass Enzyme kinetics Fermentation Fibers Manganese compounds Endoglucanase Endoxylanase Fomes sclerodermeus Laccase Manganese peroxidase (MnP) Polygalacturonase Enzymes Fomes Triticum aestivum To investigate the growth and production of lignocellulosic enzymes from Fomes sclerodermeus, solid-state fermentation (SSF) was performed by using soy and wheat bran. Both media showed similar maximal values of dry weight loss of the substrate and biomass production, nevertheless enzyme production was markedly different according to the substrate used. Cellulases, xylanases and pectinases in soy bran medium reached the highest activity at 12 days of fermentation, while maximal manganese peroxidase (MnP) activity occurred at 15 days post-inoculation (14.5 U g-1), laccase peaked (520 U g-1) at the last sampling day (28). Using the medium with a mixture (1:1) of wheat and soy bran, the hydrolases reached the maximum value at 15 days being the amounts of pectinases produced 3-fold higher than those obtained using soy bran as substrate. However, the amounts of ligninases were lower, the values measured for MnP and laccase were 4.64 and 66.7 U g-1, respectively. Therefore, the high enzyme production along with the very low cost of the substrate, showed the suitability of the system F. sclerodermeus-SSF for industrial purposes, being the enzyme production different for each enzymatic system according to substrate composition. © 2006 Elsevier Ltd. All rights reserved. Fil:Papinutti, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v81_n1_p54_Papinutti http://hdl.handle.net/20.500.12110/paper_02608774_v81_n1_p54_Papinutti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Endoglucanase
Endoxylanase
Fomes sclerodermeus
Laccase
Manganese peroxidase
Polygalacturonase
Biomass
Enzyme kinetics
Fermentation
Fibers
Manganese compounds
Endoglucanase
Endoxylanase
Fomes sclerodermeus
Laccase
Manganese peroxidase (MnP)
Polygalacturonase
Enzymes
Fomes
Triticum aestivum
spellingShingle Endoglucanase
Endoxylanase
Fomes sclerodermeus
Laccase
Manganese peroxidase
Polygalacturonase
Biomass
Enzyme kinetics
Fermentation
Fibers
Manganese compounds
Endoglucanase
Endoxylanase
Fomes sclerodermeus
Laccase
Manganese peroxidase (MnP)
Polygalacturonase
Enzymes
Fomes
Triticum aestivum
Papinutti, Víctor Leandro
Forchíassin, Flavia
Lignocellulolytic enzymes from Fomes sclerodermeus growing in solid-state fermentation
topic_facet Endoglucanase
Endoxylanase
Fomes sclerodermeus
Laccase
Manganese peroxidase
Polygalacturonase
Biomass
Enzyme kinetics
Fermentation
Fibers
Manganese compounds
Endoglucanase
Endoxylanase
Fomes sclerodermeus
Laccase
Manganese peroxidase (MnP)
Polygalacturonase
Enzymes
Fomes
Triticum aestivum
description To investigate the growth and production of lignocellulosic enzymes from Fomes sclerodermeus, solid-state fermentation (SSF) was performed by using soy and wheat bran. Both media showed similar maximal values of dry weight loss of the substrate and biomass production, nevertheless enzyme production was markedly different according to the substrate used. Cellulases, xylanases and pectinases in soy bran medium reached the highest activity at 12 days of fermentation, while maximal manganese peroxidase (MnP) activity occurred at 15 days post-inoculation (14.5 U g-1), laccase peaked (520 U g-1) at the last sampling day (28). Using the medium with a mixture (1:1) of wheat and soy bran, the hydrolases reached the maximum value at 15 days being the amounts of pectinases produced 3-fold higher than those obtained using soy bran as substrate. However, the amounts of ligninases were lower, the values measured for MnP and laccase were 4.64 and 66.7 U g-1, respectively. Therefore, the high enzyme production along with the very low cost of the substrate, showed the suitability of the system F. sclerodermeus-SSF for industrial purposes, being the enzyme production different for each enzymatic system according to substrate composition. © 2006 Elsevier Ltd. All rights reserved.
author Papinutti, Víctor Leandro
Forchíassin, Flavia
author_facet Papinutti, Víctor Leandro
Forchíassin, Flavia
author_sort Papinutti, Víctor Leandro
title Lignocellulolytic enzymes from Fomes sclerodermeus growing in solid-state fermentation
title_short Lignocellulolytic enzymes from Fomes sclerodermeus growing in solid-state fermentation
title_full Lignocellulolytic enzymes from Fomes sclerodermeus growing in solid-state fermentation
title_fullStr Lignocellulolytic enzymes from Fomes sclerodermeus growing in solid-state fermentation
title_full_unstemmed Lignocellulolytic enzymes from Fomes sclerodermeus growing in solid-state fermentation
title_sort lignocellulolytic enzymes from fomes sclerodermeus growing in solid-state fermentation
publishDate 2007
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v81_n1_p54_Papinutti
http://hdl.handle.net/20.500.12110/paper_02608774_v81_n1_p54_Papinutti
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AT forchiassinflavia lignocellulolyticenzymesfromfomessclerodermeusgrowinginsolidstatefermentation
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