β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation
The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and fi...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v131_n_p65_Carpineti http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti |
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paper:paper_02608774_v131_n_p65_Carpineti2023-06-08T15:22:19Z β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation Martínez, María Julia Pilosof, Ana María Renata Pérez, Oscar E. β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core-shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core-shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations. © 2014 Elsevier Ltd. All rights reserved. Fil:Martinez, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pilosof, A.M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez, O.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v131_n_p65_Carpineti http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization |
| spellingShingle |
β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization Martínez, María Julia Pilosof, Ana María Renata Pérez, Oscar E. β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation |
| topic_facet |
β-Lactoglobulin Carboximethylcellulose Core-shell microparticles Electrostatic deposition Lyophilization |
| description |
The aim of this work was to build, to isolate and to characterize, core-shell microparticles composed of a core of thermally aggregated β-lactoglobulin (β-lg) covered by a shell of carboxymethylcellulose (CMC). The core-shell particles were obtained by mixing (β-lg)n and CMC solutions at pH 7 and finally, decreasing the pH up to 4, promoting the adsorption of CMC on the protein core due their opposite electric charge. The core-shell microparticles were characterized by static laser light scattering (SLS), optical microscopy and atomic force microscopy (AFM). At pH 4, (β-lg)n showed a diameter ∼200 nm, but after adding the polysaccharide had a diameter ∼1 μm. The microscopy corroborated the data obtained by SLS measurements. Core-shell microparticles could be isolated by lyophilization and potentially applied as a fat replacement and/or a delivery systems for encapsulated substances in food formulations. © 2014 Elsevier Ltd. All rights reserved. |
| author |
Martínez, María Julia Pilosof, Ana María Renata Pérez, Oscar E. |
| author_facet |
Martínez, María Julia Pilosof, Ana María Renata Pérez, Oscar E. |
| author_sort |
Martínez, María Julia |
| title |
β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation |
| title_short |
β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation |
| title_full |
β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation |
| title_fullStr |
β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation |
| title_full_unstemmed |
β-Lactoglobulin-carboxymethylcellulose core-shell microparticles: Construction, characterization and isolation |
| title_sort |
β-lactoglobulin-carboxymethylcellulose core-shell microparticles: construction, characterization and isolation |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_02608774_v131_n_p65_Carpineti http://hdl.handle.net/20.500.12110/paper_02608774_v131_n_p65_Carpineti |
| work_keys_str_mv |
AT martinezmariajulia blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation AT pilosofanamariarenata blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation AT perezoscare blactoglobulincarboxymethylcellulosecoreshellmicroparticlesconstructioncharacterizationandisolation |
| _version_ |
1768543417775685632 |