Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions

Aggregation is a common trait of bacteria in natural and engineered biological systems. Microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. It is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmen...

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Autores principales:	Mazzella, María Agustina, Erijman, Leonardo
Publicado:	2015
Materias:	Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0233111X_v55_n9_p1134_Ayarza http://hdl.handle.net/20.500.12110/paper_0233111X_v55_n9_p1134_Ayarza
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0233111X_v55_n9_p1134_Ayarza
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spelling	paper:paper_0233111X_v55_n9_p1134_Ayarza2023-06-08T15:21:49Z Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions Mazzella, María Agustina Erijman, Leonardo Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes culture medium isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Bacteroidetes Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome Aggregation is a common trait of bacteria in natural and engineered biological systems. Microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. It is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmental stress better than free-swimming bacteria of the same species. We performed a proteomic analysis of a strain of Sediminibacterium, isolated from activated sludge, which grew planktonically in diluted culture media and in an aggregated form in media containing a high concentration of organic substrate. Auto-aggregation was also observed in the presence of pyruvate in dilute media. Expression of a number of stress-related proteins significantly increased under planktonic growth in comparison to aggregate growth. The upregulated proteins, identified by MALDI-TOF mass spectrometry, were two isoforms of a protein belonging to the universal stress family (UspA), a thioredoxin-disulfide reductase, the Campylobacter jejuni orthologue transcriptional regulator (Cj1172c), and the CocE/NonD hydrolase. We conclude that Sediminibaterium sp. C3 growth is stressed under planktonic conditions and that aggregation induced by pyruvate protects the bacteria against oxidative stress. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Fil:Mazzella, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0233111X_v55_n9_p1134_Ayarza http://hdl.handle.net/20.500.12110/paper_0233111X_v55_n9_p1134_Ayarza
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes culture medium isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Bacteroidetes Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome
spellingShingle	Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes culture medium isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Bacteroidetes Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome Mazzella, María Agustina Erijman, Leonardo Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions
topic_facet	Auto-aggregation Microaerophily Proteomics Stress bacterial protein culture medium heat shock protein hydrolase pyruvic acid thioredoxin reductase transcription factor transcriptome Bacteroidetes culture medium isolation and purification mass spectrometry metabolism oxidative stress physiology Bacterial Proteins Bacteroidetes Culture Media Heat-Shock Proteins Hydrolases Oxidative Stress Pyruvic Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thioredoxin-Disulfide Reductase Transcription Factors Transcriptome
description	Aggregation is a common trait of bacteria in natural and engineered biological systems. Microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. It is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmental stress better than free-swimming bacteria of the same species. We performed a proteomic analysis of a strain of Sediminibacterium, isolated from activated sludge, which grew planktonically in diluted culture media and in an aggregated form in media containing a high concentration of organic substrate. Auto-aggregation was also observed in the presence of pyruvate in dilute media. Expression of a number of stress-related proteins significantly increased under planktonic growth in comparison to aggregate growth. The upregulated proteins, identified by MALDI-TOF mass spectrometry, were two isoforms of a protein belonging to the universal stress family (UspA), a thioredoxin-disulfide reductase, the Campylobacter jejuni orthologue transcriptional regulator (Cj1172c), and the CocE/NonD hydrolase. We conclude that Sediminibaterium sp. C3 growth is stressed under planktonic conditions and that aggregation induced by pyruvate protects the bacteria against oxidative stress. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
author	Mazzella, María Agustina Erijman, Leonardo
author_facet	Mazzella, María Agustina Erijman, Leonardo
author_sort	Mazzella, María Agustina
title	Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions
title_short	Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions
title_full	Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions
title_fullStr	Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions
title_full_unstemmed	Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions
title_sort	expression of stress-related proteins in sediminibacterium sp. growing under planktonic conditions
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0233111X_v55_n9_p1134_Ayarza http://hdl.handle.net/20.500.12110/paper_0233111X_v55_n9_p1134_Ayarza
work_keys_str_mv	AT mazzellamariaagustina expressionofstressrelatedproteinsinsediminibacteriumspgrowingunderplanktonicconditions AT erijmanleonardo expressionofstressrelatedproteinsinsediminibacteriumspgrowingunderplanktonicconditions
_version_	1768544315425947648

Expression of stress-related proteins in Sediminibacterium sp. growing under planktonic conditions

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