Characterization and distribution of Citrobacter strains in a University Hospital
OBJETIVE. a) To identify Citrobacter strains following the conventional biochemical reaction of Brenner and col; b) to evaluate the sensitivity and specificity of the O'Hara's method compared with Brenner's method, and c) to determine the rate and distribution of the strains in the cl...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0213005X_v19_n1_p11_Manganello http://hdl.handle.net/20.500.12110/paper_0213005X_v19_n1_p11_Manganello |
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paper:paper_0213005X_v19_n1_p11_Manganello2023-06-08T15:20:46Z Characterization and distribution of Citrobacter strains in a University Hospital Citrobacter Distributions Identification bacterial protein enzyme article bacteremia carbohydrate metabolism Citrobacter Citrobacter freundii classification communicable disease cross infection Enterobacter infection feces human isolation and purification metabolism methodology microbiological examination microbiology opportunistic infection retrospective study sensitivity and specificity Spain species difference statistics surgical infection university hospital urinary tract infection Bacteremia Bacterial Proteins Bacteriological Techniques Carbohydrate Metabolism Citrobacter Citrobacter freundii Classification Community-Acquired Infections Cross Infection Enterobacteriaceae Infections Enzymes Feces Hospitals, University Humans Opportunistic Infections Retrospective Studies Sensitivity and Specificity Spain Species Specificity Surgical Wound Infection Urinary Tract Infections OBJETIVE. a) To identify Citrobacter strains following the conventional biochemical reaction of Brenner and col; b) to evaluate the sensitivity and specificity of the O'Hara's method compared with Brenner's method, and c) to determine the rate and distribution of the strains in the clinical isolates. MATERIAL AND METHODS. One hundred and twenty two clinical isolates, characterized as Citrobacter spp. were collected between May of 1994 and August of 1997. Clinical isolates included impatients and outpatients from Hospital de Clinicas. Strains were identified following the methods of Brenner and O'Hara. RESULTS. Methods of Brenner identified 111 of 122 strains: C. freundii 59 of 111; C. koseri 18 of 111; C. werkmanii 15 of 111; C. braakii 9 of 111; C. youngae 6 of 111 and C. amalonaticus 4 of 111. O'Hara's methods identified 104 of 111 strains (94%). C. freundii was recovered most frequently from urine and feces (p Fisher <0.026 and 0.039 respectively), while C. koseri was isolated from urine principally (p Fisher <0.0372). CONCLUSIONS. The genus Citrobacter is and important oportunistic pathogen that can be identified in clinical microbiology's laboratories using O'Hara's method. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0213005X_v19_n1_p11_Manganello http://hdl.handle.net/20.500.12110/paper_0213005X_v19_n1_p11_Manganello |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Citrobacter Distributions Identification bacterial protein enzyme article bacteremia carbohydrate metabolism Citrobacter Citrobacter freundii classification communicable disease cross infection Enterobacter infection feces human isolation and purification metabolism methodology microbiological examination microbiology opportunistic infection retrospective study sensitivity and specificity Spain species difference statistics surgical infection university hospital urinary tract infection Bacteremia Bacterial Proteins Bacteriological Techniques Carbohydrate Metabolism Citrobacter Citrobacter freundii Classification Community-Acquired Infections Cross Infection Enterobacteriaceae Infections Enzymes Feces Hospitals, University Humans Opportunistic Infections Retrospective Studies Sensitivity and Specificity Spain Species Specificity Surgical Wound Infection Urinary Tract Infections |
| spellingShingle |
Citrobacter Distributions Identification bacterial protein enzyme article bacteremia carbohydrate metabolism Citrobacter Citrobacter freundii classification communicable disease cross infection Enterobacter infection feces human isolation and purification metabolism methodology microbiological examination microbiology opportunistic infection retrospective study sensitivity and specificity Spain species difference statistics surgical infection university hospital urinary tract infection Bacteremia Bacterial Proteins Bacteriological Techniques Carbohydrate Metabolism Citrobacter Citrobacter freundii Classification Community-Acquired Infections Cross Infection Enterobacteriaceae Infections Enzymes Feces Hospitals, University Humans Opportunistic Infections Retrospective Studies Sensitivity and Specificity Spain Species Specificity Surgical Wound Infection Urinary Tract Infections Characterization and distribution of Citrobacter strains in a University Hospital |
| topic_facet |
Citrobacter Distributions Identification bacterial protein enzyme article bacteremia carbohydrate metabolism Citrobacter Citrobacter freundii classification communicable disease cross infection Enterobacter infection feces human isolation and purification metabolism methodology microbiological examination microbiology opportunistic infection retrospective study sensitivity and specificity Spain species difference statistics surgical infection university hospital urinary tract infection Bacteremia Bacterial Proteins Bacteriological Techniques Carbohydrate Metabolism Citrobacter Citrobacter freundii Classification Community-Acquired Infections Cross Infection Enterobacteriaceae Infections Enzymes Feces Hospitals, University Humans Opportunistic Infections Retrospective Studies Sensitivity and Specificity Spain Species Specificity Surgical Wound Infection Urinary Tract Infections |
| description |
OBJETIVE. a) To identify Citrobacter strains following the conventional biochemical reaction of Brenner and col; b) to evaluate the sensitivity and specificity of the O'Hara's method compared with Brenner's method, and c) to determine the rate and distribution of the strains in the clinical isolates. MATERIAL AND METHODS. One hundred and twenty two clinical isolates, characterized as Citrobacter spp. were collected between May of 1994 and August of 1997. Clinical isolates included impatients and outpatients from Hospital de Clinicas. Strains were identified following the methods of Brenner and O'Hara. RESULTS. Methods of Brenner identified 111 of 122 strains: C. freundii 59 of 111; C. koseri 18 of 111; C. werkmanii 15 of 111; C. braakii 9 of 111; C. youngae 6 of 111 and C. amalonaticus 4 of 111. O'Hara's methods identified 104 of 111 strains (94%). C. freundii was recovered most frequently from urine and feces (p Fisher <0.026 and 0.039 respectively), while C. koseri was isolated from urine principally (p Fisher <0.0372). CONCLUSIONS. The genus Citrobacter is and important oportunistic pathogen that can be identified in clinical microbiology's laboratories using O'Hara's method. |
| title |
Characterization and distribution of Citrobacter strains in a University Hospital |
| title_short |
Characterization and distribution of Citrobacter strains in a University Hospital |
| title_full |
Characterization and distribution of Citrobacter strains in a University Hospital |
| title_fullStr |
Characterization and distribution of Citrobacter strains in a University Hospital |
| title_full_unstemmed |
Characterization and distribution of Citrobacter strains in a University Hospital |
| title_sort |
characterization and distribution of citrobacter strains in a university hospital |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0213005X_v19_n1_p11_Manganello http://hdl.handle.net/20.500.12110/paper_0213005X_v19_n1_p11_Manganello |
| _version_ |
1768545185763950592 |