Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle
In the CNS, transferrin (Tf) is expressed by the oligodendroglial cells (OLGcs) and is essential for their development. We have previously shown that apotransferrin (aTf) accelerates maturation of OLGcs in vivo as well as in vitro. The mechanisms involved in this action appear to be complex and have...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01970186_v49_n4_p359_Paez http://hdl.handle.net/20.500.12110/paper_01970186_v49_n4_p359_Paez |
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paper:paper_01970186_v49_n4_p359_Paez2023-06-08T15:20:34Z Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle Cell cycle Cyclins Oligodendrocyte PDGF Transferrin apotransferrin cyclin D cyclin dependent kinase 2 cyclin dependent kinase 4 cyclin E protein p27 article cell cycle cell cycle G1 phase cell cycle S phase cell differentiation cell maturation cell structure central nervous system controlled study evaluation flow cytometry human human cell oligodendroglia priority journal protein expression stem cell Animals Antimetabolites Apoproteins Blotting, Western Bromodeoxyuridine Cell Cycle Cell Differentiation Cyclin-Dependent Kinases Depression, Chemical DNA Electrophoresis, Polyacrylamide Gel Flow Cytometry G1 Phase Immunohistochemistry Oligodendroglia Platelet-Derived Growth Factor Rats S Phase Stem Cells Tetrazolium Salts Thiazoles Transferrin In the CNS, transferrin (Tf) is expressed by the oligodendroglial cells (OLGcs) and is essential for their development. We have previously shown that apotransferrin (aTf) accelerates maturation of OLGcs in vivo as well as in vitro. The mechanisms involved in this action appear to be complex and have not been completely elucidated. The aim of this study was to investigate if Tf participates in the regulation of the cell cycle of oligodendroglial progenitor cells (OPcs). Primary cultures of OPcs were treated with aTf and/or with different combinations of mitogenic factors. Cell cycle progression was studied by BrdU incorporation, flow cytometry and by the expression of cell cycle regulatory proteins. Apotransferrin decreased the number of BrdU+ cells, increasing the cell cycle time and decreasing the number of cells in S phase. The cell cycle inhibitors p27kip1, p21cip1 and p53 were increased, and in agreement with these results, the activity of the complexes involved in G1-S progression (cyclin D/CDK4, cyclin E/CDK2), was dramatically decreased. Apotransferrin also inhibited the mitogenic effects of PDGF and PDGF/IGF on OPcs, but did not affect their proliferation rate in the presence of bFGF, bFGF/PDGF or bFGF/IGF. Our results indicate that inhibition of the progression of the cell cycle of OPcs by aTf, even in the presence of PDGF, leads to an early beginning of the differentiation program, evaluated by different maturation markers (O4, GC and MBP) and by morphological criteria. The modulation by aTf of the response of OPcs to PDGF supports the idea that this glycoprotein might act as a key regulator of the OLGc lineage progression. © 2006 Elsevier Ltd. All rights reserved. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01970186_v49_n4_p359_Paez http://hdl.handle.net/20.500.12110/paper_01970186_v49_n4_p359_Paez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell cycle Cyclins Oligodendrocyte PDGF Transferrin apotransferrin cyclin D cyclin dependent kinase 2 cyclin dependent kinase 4 cyclin E protein p27 article cell cycle cell cycle G1 phase cell cycle S phase cell differentiation cell maturation cell structure central nervous system controlled study evaluation flow cytometry human human cell oligodendroglia priority journal protein expression stem cell Animals Antimetabolites Apoproteins Blotting, Western Bromodeoxyuridine Cell Cycle Cell Differentiation Cyclin-Dependent Kinases Depression, Chemical DNA Electrophoresis, Polyacrylamide Gel Flow Cytometry G1 Phase Immunohistochemistry Oligodendroglia Platelet-Derived Growth Factor Rats S Phase Stem Cells Tetrazolium Salts Thiazoles Transferrin |
| spellingShingle |
Cell cycle Cyclins Oligodendrocyte PDGF Transferrin apotransferrin cyclin D cyclin dependent kinase 2 cyclin dependent kinase 4 cyclin E protein p27 article cell cycle cell cycle G1 phase cell cycle S phase cell differentiation cell maturation cell structure central nervous system controlled study evaluation flow cytometry human human cell oligodendroglia priority journal protein expression stem cell Animals Antimetabolites Apoproteins Blotting, Western Bromodeoxyuridine Cell Cycle Cell Differentiation Cyclin-Dependent Kinases Depression, Chemical DNA Electrophoresis, Polyacrylamide Gel Flow Cytometry G1 Phase Immunohistochemistry Oligodendroglia Platelet-Derived Growth Factor Rats S Phase Stem Cells Tetrazolium Salts Thiazoles Transferrin Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle |
| topic_facet |
Cell cycle Cyclins Oligodendrocyte PDGF Transferrin apotransferrin cyclin D cyclin dependent kinase 2 cyclin dependent kinase 4 cyclin E protein p27 article cell cycle cell cycle G1 phase cell cycle S phase cell differentiation cell maturation cell structure central nervous system controlled study evaluation flow cytometry human human cell oligodendroglia priority journal protein expression stem cell Animals Antimetabolites Apoproteins Blotting, Western Bromodeoxyuridine Cell Cycle Cell Differentiation Cyclin-Dependent Kinases Depression, Chemical DNA Electrophoresis, Polyacrylamide Gel Flow Cytometry G1 Phase Immunohistochemistry Oligodendroglia Platelet-Derived Growth Factor Rats S Phase Stem Cells Tetrazolium Salts Thiazoles Transferrin |
| description |
In the CNS, transferrin (Tf) is expressed by the oligodendroglial cells (OLGcs) and is essential for their development. We have previously shown that apotransferrin (aTf) accelerates maturation of OLGcs in vivo as well as in vitro. The mechanisms involved in this action appear to be complex and have not been completely elucidated. The aim of this study was to investigate if Tf participates in the regulation of the cell cycle of oligodendroglial progenitor cells (OPcs). Primary cultures of OPcs were treated with aTf and/or with different combinations of mitogenic factors. Cell cycle progression was studied by BrdU incorporation, flow cytometry and by the expression of cell cycle regulatory proteins. Apotransferrin decreased the number of BrdU+ cells, increasing the cell cycle time and decreasing the number of cells in S phase. The cell cycle inhibitors p27kip1, p21cip1 and p53 were increased, and in agreement with these results, the activity of the complexes involved in G1-S progression (cyclin D/CDK4, cyclin E/CDK2), was dramatically decreased. Apotransferrin also inhibited the mitogenic effects of PDGF and PDGF/IGF on OPcs, but did not affect their proliferation rate in the presence of bFGF, bFGF/PDGF or bFGF/IGF. Our results indicate that inhibition of the progression of the cell cycle of OPcs by aTf, even in the presence of PDGF, leads to an early beginning of the differentiation program, evaluated by different maturation markers (O4, GC and MBP) and by morphological criteria. The modulation by aTf of the response of OPcs to PDGF supports the idea that this glycoprotein might act as a key regulator of the OLGc lineage progression. © 2006 Elsevier Ltd. All rights reserved. |
| title |
Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle |
| title_short |
Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle |
| title_full |
Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle |
| title_fullStr |
Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle |
| title_full_unstemmed |
Apotransferrin decreases the response of oligodendrocyte progenitors to PDGF and inhibits the progression of the cell cycle |
| title_sort |
apotransferrin decreases the response of oligodendrocyte progenitors to pdgf and inhibits the progression of the cell cycle |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01970186_v49_n4_p359_Paez http://hdl.handle.net/20.500.12110/paper_01970186_v49_n4_p359_Paez |
| _version_ |
1768546206307319808 |