Effects of Prolactin on Prostate Androgen Receptors in Male Rats

Five groups of immature (30‐day‐old) intact male rats were injected daily for ten days with different doses of ovine prolactin (5, 10, 25, 50, or 500 μg per rat per day). At the end of the treatment period, the animals were killed, serum was collected for LH and androgens measurements and weights of...

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Autores principales:	Barañao, José Lino S., Tesone, Marta, Calvo, Juan Carlos, Charreau, Eduardo Hernán
Publicado:	1982
Materias:	androgen receptors immature male rats prolactin prostate
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01963635_v3_n5_p281_BARANAO http://hdl.handle.net/20.500.12110/paper_01963635_v3_n5_p281_BARANAO
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01963635_v3_n5_p281_BARANAO
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spelling	paper:paper_01963635_v3_n5_p281_BARANAO2023-06-08T15:20:29Z Effects of Prolactin on Prostate Androgen Receptors in Male Rats Barañao, José Lino S. Tesone, Marta Calvo, Juan Carlos Charreau, Eduardo Hernán androgen receptors immature male rats prolactin prostate Five groups of immature (30‐day‐old) intact male rats were injected daily for ten days with different doses of ovine prolactin (5, 10, 25, 50, or 500 μg per rat per day). At the end of the treatment period, the animals were killed, serum was collected for LH and androgens measurements and weights of testes, prostates, and epididymides were recorded. Available and exchangeable cytoplasmic and total nuclear KC1‐extractable androgen binding sites in the prostate gland were determined using the synthetic androgen R1881 as radioligand. A significant increase in the weights of testes and accessory reproductive glands was observed with the highest dose injected (500 μg per rat per day) as follows: testes, 583 ± 21 (SEM) versus 448 ± 38 mg (P <0.05); prostate, 80.0 ± 4.0 versus 64.7 ± 3.1 mg (P <0.05); epididymis, 72.1 ± 5.0 versus 58.2 ± 4.0 mg (P <0.05). Serum levels of LH were significantly higher in prolactin‐treated animals. Maximal values of serum LH were attained with the dose of 50 μg (99.0 ± 11.2 versus 32.0 ± 4.6 ng/ml; P < 0.05). Increasing doses of prolactin produced a concomitant rise in serum 3α‐androstanediol (2.21 ± 0.45 versus 6.63 ± 0.42 ng/ml in the group treated with 50 μg), whereas testosterone plus dihydrotestosterone levels remained unchanged. A decrease in the concentration of exchangeable cytosolic androgen binding sites in the prostate (fmoleslmg protein) was observed in prolactin injected animals (88.2 ± 0.8 in the control group versus 80.6 ± 0.3 and 74.9 ± 0.3 for doses of 5 and 50 μg, respectively; P <0.05). No changes in androgen binding sites were detected in the group treated with 500 μg per rat per day. On the other hand, prolactin treatment did not alter the number of available androgen binding sites in the prostate gland. Increasing doses of prolactin induced a dose‐related increase in the total number of nuclear KC1‐extractable receptors, expressed per mg DNA or per organ. In addition, the total content of androgen receptors (cytosolic plus nuclear binding sites) was significantly higher in animals treated with 500 μg of prolactin (385 ± 34 versus 228 ± 37 fmoles per organ; P < 0.05). These results suggest that, in addition to the previously described effects of prolactin on Leydig cell responsiveness to LH and on androgen metabolism, this hormone could also modify peripheral LH and androgen levels as well as the androgen receptor content in the rat prostate gland. 1982 American Society of Andrology Fil:BARAÑAO, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:TESONE, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:CALVO, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:CHARREAU, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1982 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01963635_v3_n5_p281_BARANAO http://hdl.handle.net/20.500.12110/paper_01963635_v3_n5_p281_BARANAO
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	androgen receptors immature male rats prolactin prostate
spellingShingle	androgen receptors immature male rats prolactin prostate Barañao, José Lino S. Tesone, Marta Calvo, Juan Carlos Charreau, Eduardo Hernán Effects of Prolactin on Prostate Androgen Receptors in Male Rats
topic_facet	androgen receptors immature male rats prolactin prostate
description	Five groups of immature (30‐day‐old) intact male rats were injected daily for ten days with different doses of ovine prolactin (5, 10, 25, 50, or 500 μg per rat per day). At the end of the treatment period, the animals were killed, serum was collected for LH and androgens measurements and weights of testes, prostates, and epididymides were recorded. Available and exchangeable cytoplasmic and total nuclear KC1‐extractable androgen binding sites in the prostate gland were determined using the synthetic androgen R1881 as radioligand. A significant increase in the weights of testes and accessory reproductive glands was observed with the highest dose injected (500 μg per rat per day) as follows: testes, 583 ± 21 (SEM) versus 448 ± 38 mg (P <0.05); prostate, 80.0 ± 4.0 versus 64.7 ± 3.1 mg (P <0.05); epididymis, 72.1 ± 5.0 versus 58.2 ± 4.0 mg (P <0.05). Serum levels of LH were significantly higher in prolactin‐treated animals. Maximal values of serum LH were attained with the dose of 50 μg (99.0 ± 11.2 versus 32.0 ± 4.6 ng/ml; P < 0.05). Increasing doses of prolactin produced a concomitant rise in serum 3α‐androstanediol (2.21 ± 0.45 versus 6.63 ± 0.42 ng/ml in the group treated with 50 μg), whereas testosterone plus dihydrotestosterone levels remained unchanged. A decrease in the concentration of exchangeable cytosolic androgen binding sites in the prostate (fmoleslmg protein) was observed in prolactin injected animals (88.2 ± 0.8 in the control group versus 80.6 ± 0.3 and 74.9 ± 0.3 for doses of 5 and 50 μg, respectively; P <0.05). No changes in androgen binding sites were detected in the group treated with 500 μg per rat per day. On the other hand, prolactin treatment did not alter the number of available androgen binding sites in the prostate gland. Increasing doses of prolactin induced a dose‐related increase in the total number of nuclear KC1‐extractable receptors, expressed per mg DNA or per organ. In addition, the total content of androgen receptors (cytosolic plus nuclear binding sites) was significantly higher in animals treated with 500 μg of prolactin (385 ± 34 versus 228 ± 37 fmoles per organ; P < 0.05). These results suggest that, in addition to the previously described effects of prolactin on Leydig cell responsiveness to LH and on androgen metabolism, this hormone could also modify peripheral LH and androgen levels as well as the androgen receptor content in the rat prostate gland. 1982 American Society of Andrology
author	Barañao, José Lino S. Tesone, Marta Calvo, Juan Carlos Charreau, Eduardo Hernán
author_facet	Barañao, José Lino S. Tesone, Marta Calvo, Juan Carlos Charreau, Eduardo Hernán
author_sort	Barañao, José Lino S.
title	Effects of Prolactin on Prostate Androgen Receptors in Male Rats
title_short	Effects of Prolactin on Prostate Androgen Receptors in Male Rats
title_full	Effects of Prolactin on Prostate Androgen Receptors in Male Rats
title_fullStr	Effects of Prolactin on Prostate Androgen Receptors in Male Rats
title_full_unstemmed	Effects of Prolactin on Prostate Androgen Receptors in Male Rats
title_sort	effects of prolactin on prostate androgen receptors in male rats
publishDate	1982
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01963635_v3_n5_p281_BARANAO http://hdl.handle.net/20.500.12110/paper_01963635_v3_n5_p281_BARANAO
work_keys_str_mv	AT baranaojoselinos effectsofprolactinonprostateandrogenreceptorsinmalerats AT tesonemarta effectsofprolactinonprostateandrogenreceptorsinmalerats AT calvojuancarlos effectsofprolactinonprostateandrogenreceptorsinmalerats AT charreaueduardohernan effectsofprolactinonprostateandrogenreceptorsinmalerats
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Effects of Prolactin on Prostate Androgen Receptors in Male Rats

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