Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells
We studied the effects of ANG II on extracellular signal-regulated kinase (ERK)1/2 phosphorylation in rat pituitary cells. ANG II increased ERK phosphorylation in a time- and concentration-dependent way. Maximum effect was obtained at 5 min at a concentration of 10-100 nM. The effect of 100 nM ANG I...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v285_n348-3_pE645_Suarez http://hdl.handle.net/20.500.12110/paper_01931849_v285_n348-3_pE645_Suarez |
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paper:paper_01931849_v285_n348-3_pE645_Suarez2023-06-08T15:20:03Z Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells Calcium Epidermal growth factor receptor Estrogen Mitogen-activated protein kinase Phospholipase C Protein kinase C 1 [[6 (3 methoxyestra 1,3,5(10) trien 17beta yl)amino]hexyl] 1h pyrrole 2,5 dione 2 (2 amino 3 methoxyphenyl)chromone 3,4,5 trimethoxybenzoic acid 8 (diethylamino)octyl ester 4 (3 chloroanilino) 6,7 dimethoxyquinazoline angiotensin egtazic acid epidermal growth factor receptor ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid herbimycin losartan potassium mitogen activated protein kinase mitogen activated protein kinase 1 pertussis toxin protein tyrosine kinase inhibitor adenohypophysis animal cell animal experiment article calcium cell level calcium mobilization cell hyperplasia concentration response controlled study enzyme activation enzyme activity enzyme inhibition enzyme phosphorylation female hypophysis cell nonhuman phosphorylation priority journal rat Angiotensin II Animals Calcium Cells, Cultured Estrogens Female Hyperplasia Mitogen-Activated Protein Kinases Phospholipase C Phosphorylation Pituitary Gland, Anterior Protein Kinase C Rats Rats, Sprague-Dawley Receptor, Angiotensin, Type 1 Receptor, Epidermal Growth Factor Receptors, Angiotensin src-Family Kinases Vasoconstrictor Agents We studied the effects of ANG II on extracellular signal-regulated kinase (ERK)1/2 phosphorylation in rat pituitary cells. ANG II increased ERK phosphorylation in a time- and concentration-dependent way. Maximum effect was obtained at 5 min at a concentration of 10-100 nM. The effect of 100 nM ANG II was blocked by the AT1 antagonist DUP-753, by the phospholipase C (PLC) inhibitor U-73122, and by the MAPK kinase (MEK) antagonist PD-98059. The ANG II-induced increase in phosphorylated (p)ERK was insensitive to pertussis toxin blockade and PKC depletion or inhibition. The effect was also abrogated by chelating intracellular calcium with BAPTA-AM or TMB-8 by depleting intracellular calcium stores with a 30-min pretreatment with EGTA and by pretreatment with herbimycin A and PP1, two c-Src tyrosine kinase inhibitors. It was attenuated by AG-1478, an inhibitor of epidermal growth factor receptor (EGFR) activation. Therefore, in the rat pituitary, the increase of pERK is a Gq- and PLC-dependent process, which involves an increase in intracellular calcium and activation of a c-Src tyrosine kinase, transactivation of the EGFR, and the activation of MEK. Finally, the response of ERK activation by ANG II is altered in hyperplastic pituitary cells, in which calcium mobilization evoked by ANG II is also modified. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v285_n348-3_pE645_Suarez http://hdl.handle.net/20.500.12110/paper_01931849_v285_n348-3_pE645_Suarez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Calcium Epidermal growth factor receptor Estrogen Mitogen-activated protein kinase Phospholipase C Protein kinase C 1 [[6 (3 methoxyestra 1,3,5(10) trien 17beta yl)amino]hexyl] 1h pyrrole 2,5 dione 2 (2 amino 3 methoxyphenyl)chromone 3,4,5 trimethoxybenzoic acid 8 (diethylamino)octyl ester 4 (3 chloroanilino) 6,7 dimethoxyquinazoline angiotensin egtazic acid epidermal growth factor receptor ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid herbimycin losartan potassium mitogen activated protein kinase mitogen activated protein kinase 1 pertussis toxin protein tyrosine kinase inhibitor adenohypophysis animal cell animal experiment article calcium cell level calcium mobilization cell hyperplasia concentration response controlled study enzyme activation enzyme activity enzyme inhibition enzyme phosphorylation female hypophysis cell nonhuman phosphorylation priority journal rat Angiotensin II Animals Calcium Cells, Cultured Estrogens Female Hyperplasia Mitogen-Activated Protein Kinases Phospholipase C Phosphorylation Pituitary Gland, Anterior Protein Kinase C Rats Rats, Sprague-Dawley Receptor, Angiotensin, Type 1 Receptor, Epidermal Growth Factor Receptors, Angiotensin src-Family Kinases Vasoconstrictor Agents |
| spellingShingle |
Calcium Epidermal growth factor receptor Estrogen Mitogen-activated protein kinase Phospholipase C Protein kinase C 1 [[6 (3 methoxyestra 1,3,5(10) trien 17beta yl)amino]hexyl] 1h pyrrole 2,5 dione 2 (2 amino 3 methoxyphenyl)chromone 3,4,5 trimethoxybenzoic acid 8 (diethylamino)octyl ester 4 (3 chloroanilino) 6,7 dimethoxyquinazoline angiotensin egtazic acid epidermal growth factor receptor ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid herbimycin losartan potassium mitogen activated protein kinase mitogen activated protein kinase 1 pertussis toxin protein tyrosine kinase inhibitor adenohypophysis animal cell animal experiment article calcium cell level calcium mobilization cell hyperplasia concentration response controlled study enzyme activation enzyme activity enzyme inhibition enzyme phosphorylation female hypophysis cell nonhuman phosphorylation priority journal rat Angiotensin II Animals Calcium Cells, Cultured Estrogens Female Hyperplasia Mitogen-Activated Protein Kinases Phospholipase C Phosphorylation Pituitary Gland, Anterior Protein Kinase C Rats Rats, Sprague-Dawley Receptor, Angiotensin, Type 1 Receptor, Epidermal Growth Factor Receptors, Angiotensin src-Family Kinases Vasoconstrictor Agents Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells |
| topic_facet |
Calcium Epidermal growth factor receptor Estrogen Mitogen-activated protein kinase Phospholipase C Protein kinase C 1 [[6 (3 methoxyestra 1,3,5(10) trien 17beta yl)amino]hexyl] 1h pyrrole 2,5 dione 2 (2 amino 3 methoxyphenyl)chromone 3,4,5 trimethoxybenzoic acid 8 (diethylamino)octyl ester 4 (3 chloroanilino) 6,7 dimethoxyquinazoline angiotensin egtazic acid epidermal growth factor receptor ethylene glycol 1,2 bis(2 aminophenyl) ether n,n,n',n' tetraacetic acid herbimycin losartan potassium mitogen activated protein kinase mitogen activated protein kinase 1 pertussis toxin protein tyrosine kinase inhibitor adenohypophysis animal cell animal experiment article calcium cell level calcium mobilization cell hyperplasia concentration response controlled study enzyme activation enzyme activity enzyme inhibition enzyme phosphorylation female hypophysis cell nonhuman phosphorylation priority journal rat Angiotensin II Animals Calcium Cells, Cultured Estrogens Female Hyperplasia Mitogen-Activated Protein Kinases Phospholipase C Phosphorylation Pituitary Gland, Anterior Protein Kinase C Rats Rats, Sprague-Dawley Receptor, Angiotensin, Type 1 Receptor, Epidermal Growth Factor Receptors, Angiotensin src-Family Kinases Vasoconstrictor Agents |
| description |
We studied the effects of ANG II on extracellular signal-regulated kinase (ERK)1/2 phosphorylation in rat pituitary cells. ANG II increased ERK phosphorylation in a time- and concentration-dependent way. Maximum effect was obtained at 5 min at a concentration of 10-100 nM. The effect of 100 nM ANG II was blocked by the AT1 antagonist DUP-753, by the phospholipase C (PLC) inhibitor U-73122, and by the MAPK kinase (MEK) antagonist PD-98059. The ANG II-induced increase in phosphorylated (p)ERK was insensitive to pertussis toxin blockade and PKC depletion or inhibition. The effect was also abrogated by chelating intracellular calcium with BAPTA-AM or TMB-8 by depleting intracellular calcium stores with a 30-min pretreatment with EGTA and by pretreatment with herbimycin A and PP1, two c-Src tyrosine kinase inhibitors. It was attenuated by AG-1478, an inhibitor of epidermal growth factor receptor (EGFR) activation. Therefore, in the rat pituitary, the increase of pERK is a Gq- and PLC-dependent process, which involves an increase in intracellular calcium and activation of a c-Src tyrosine kinase, transactivation of the EGFR, and the activation of MEK. Finally, the response of ERK activation by ANG II is altered in hyperplastic pituitary cells, in which calcium mobilization evoked by ANG II is also modified. |
| title |
Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells |
| title_short |
Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells |
| title_full |
Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells |
| title_fullStr |
Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells |
| title_full_unstemmed |
Angiotensin II phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells |
| title_sort |
angiotensin ii phosphorylation of extracellular signal-regulated kinases in rat anterior pituitary cells |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01931849_v285_n348-3_pE645_Suarez http://hdl.handle.net/20.500.12110/paper_01931849_v285_n348-3_pE645_Suarez |
| _version_ |
1768544406788374528 |