Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber

Steryl glucoside (SG) and acylated steryl glucoside (ASG) synthesis was investigated in amyloplast membranes from young, intermediate and mature potato tubers. The synthesis ratio SG/ASG was lowest in young tubers (3:2) and highest in mature tubers (6:1). About a 3-fold stimulation of [14C] glucose...

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Autores principales:	Catz, Diana Silvia, Tandecarz, Juana Sara
Publicado:	1985
Materias:	amyloplast membrane potato tuber development steryl glucoside and acylated steryl glucoside synthesis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v38_n3_p179_Catz http://hdl.handle.net/20.500.12110/paper_01689452_v38_n3_p179_Catz
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01689452_v38_n3_p179_Catz
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spelling	paper:paper_01689452_v38_n3_p179_Catz2023-06-08T15:18:09Z Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber Catz, Diana Silvia Tandecarz, Juana Sara Tandecarz, Juana Sara amyloplast membrane potato tuber development steryl glucoside and acylated steryl glucoside synthesis Steryl glucoside (SG) and acylated steryl glucoside (ASG) synthesis was investigated in amyloplast membranes from young, intermediate and mature potato tubers. The synthesis ratio SG/ASG was lowest in young tubers (3:2) and highest in mature tubers (6:1). About a 3-fold stimulation of [14C] glucose incorporation into a mixture of SG was observed in amyloplast membranes from mature tubers in the presence of β-sitosterol, while radioactivity incorporation in young tubers was unaffected, thus indicating that different availabilities of endogenous acceptors occur in the membranes. The enzymes involved in sterol modification exhibit a different behavior towards Triton X-100, depending on the developmental stage of the tubers. Low concentrations of the detergent (0.045%) are required to stimulate the enzymes present in young tuber membranes (2-fold). On the other,hand, 0.15% of Triton increased the enzymatic activity in mature tubers 5-fold. These results, together with those obtained after studies of pH dependence, could be related to the lipidic structure of the vesicles formed at different developmental stages of the tubers. It is concluded that the major changes in the enzymatic activities occur as a consequence of the sterol acceptors and acyl donor content during potato tuber growth. © 1985. Fil:Catz, D.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cardini, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v38_n3_p179_Catz http://hdl.handle.net/20.500.12110/paper_01689452_v38_n3_p179_Catz
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	amyloplast membrane potato tuber development steryl glucoside and acylated steryl glucoside synthesis
spellingShingle	amyloplast membrane potato tuber development steryl glucoside and acylated steryl glucoside synthesis Catz, Diana Silvia Tandecarz, Juana Sara Tandecarz, Juana Sara Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber
topic_facet	amyloplast membrane potato tuber development steryl glucoside and acylated steryl glucoside synthesis
description	Steryl glucoside (SG) and acylated steryl glucoside (ASG) synthesis was investigated in amyloplast membranes from young, intermediate and mature potato tubers. The synthesis ratio SG/ASG was lowest in young tubers (3:2) and highest in mature tubers (6:1). About a 3-fold stimulation of [14C] glucose incorporation into a mixture of SG was observed in amyloplast membranes from mature tubers in the presence of β-sitosterol, while radioactivity incorporation in young tubers was unaffected, thus indicating that different availabilities of endogenous acceptors occur in the membranes. The enzymes involved in sterol modification exhibit a different behavior towards Triton X-100, depending on the developmental stage of the tubers. Low concentrations of the detergent (0.045%) are required to stimulate the enzymes present in young tuber membranes (2-fold). On the other,hand, 0.15% of Triton increased the enzymatic activity in mature tubers 5-fold. These results, together with those obtained after studies of pH dependence, could be related to the lipidic structure of the vesicles formed at different developmental stages of the tubers. It is concluded that the major changes in the enzymatic activities occur as a consequence of the sterol acceptors and acyl donor content during potato tuber growth. © 1985.
author	Catz, Diana Silvia Tandecarz, Juana Sara Tandecarz, Juana Sara
author_facet	Catz, Diana Silvia Tandecarz, Juana Sara Tandecarz, Juana Sara
author_sort	Catz, Diana Silvia
title	Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber
title_short	Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber
title_full	Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber
title_fullStr	Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber
title_full_unstemmed	Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber
title_sort	steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber
publishDate	1985
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v38_n3_p179_Catz http://hdl.handle.net/20.500.12110/paper_01689452_v38_n3_p179_Catz
work_keys_str_mv	AT catzdianasilvia sterylglucosideandacylsterylglucosideformationintheamyloplastmembraneduringthedevelopmentofpotatotuber AT tandecarzjuanasara sterylglucosideandacylsterylglucosideformationintheamyloplastmembraneduringthedevelopmentofpotatotuber AT tandecarzjuanasara sterylglucosideandacylsterylglucosideformationintheamyloplastmembraneduringthedevelopmentofpotatotuber
_version_	1768542592881917952

Steryl glucoside and acyl steryl glucoside formation in the amyloplast membrane during the development of potato tuber

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