Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature

Lotus species are important forage legumes due to their high nutritional value and adaptability to marginal conditions. However, the dry matter production and regrowth rate of cultivable Lotus spp. is drastically reduced during colder seasons. In this work, we evaluated the chilling response of Lotu...

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Autores principales:	Calzadilla, Pablo Ignacio, Menéndez, Ana Bernardina
Publicado:	2016
Materias:	Chilling D2 protein Legumes Photoinhibition PSII ROS antioxidant reactive oxygen metabolite adaptation cold ecotype enzymology genetics Lotus (genus) metabolism oxidative stress photosynthesis Adaptation, Biological Antioxidants Cold Temperature Ecotype Loteae Oxidative Stress Photosynthesis Reactive Oxygen Species
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v250_n_p59_Calzadilla http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p59_Calzadilla
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01689452_v250_n_p59_Calzadilla
record_format	dspace
spelling	paper:paper_01689452_v250_n_p59_Calzadilla2023-06-08T15:18:09Z Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature Calzadilla, Pablo Ignacio Menéndez, Ana Bernardina Chilling D2 protein Legumes Photoinhibition PSII ROS antioxidant reactive oxygen metabolite adaptation cold ecotype enzymology genetics Lotus (genus) metabolism oxidative stress photosynthesis Adaptation, Biological Antioxidants Cold Temperature Ecotype Loteae Oxidative Stress Photosynthesis Reactive Oxygen Species Lotus species are important forage legumes due to their high nutritional value and adaptability to marginal conditions. However, the dry matter production and regrowth rate of cultivable Lotus spp. is drastically reduced during colder seasons. In this work, we evaluated the chilling response of Lotus japonicus ecotypes MG-1 and MG-20. No significant increases were observed in reactive oxygen species and nitric oxide production or in lipid peroxidation, although a chilling-induced redox imbalance was suggested through NADPH/NADP+ ratio alterations. Antioxidant enzyme catalase, ascorbate peroxidase, and superoxide dismutase activities were also measured. Superoxide dismutase, in particular the chloroplastic isoform, showed different activity for different ecotypes and treatments. Stress-induced photoinhibition also differentially influenced both ecotypes, with MG-1 more affected than MG-20. Data showed that the D2 PSII subunit was more affected than D1 after 1 d of low temperature exposure, although its protein levels recovered over the course of the experiment. Interestingly, D2 recovery was accompanied by improvements in photosynthetic parameters (Asat and Fv/Fm) and the NADPH/NADP+ ratio. Our results suggest that the D2 protein is involved in the acclimation response of L. japonicus to low temperature. This may provide a deeper insight into the chilling tolerance mechanisms of the Lotus genus. © 2016 Elsevier Ireland Ltd. Fil:Calzadilla, P.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Menéndez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v250_n_p59_Calzadilla http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p59_Calzadilla
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Chilling D2 protein Legumes Photoinhibition PSII ROS antioxidant reactive oxygen metabolite adaptation cold ecotype enzymology genetics Lotus (genus) metabolism oxidative stress photosynthesis Adaptation, Biological Antioxidants Cold Temperature Ecotype Loteae Oxidative Stress Photosynthesis Reactive Oxygen Species
spellingShingle	Chilling D2 protein Legumes Photoinhibition PSII ROS antioxidant reactive oxygen metabolite adaptation cold ecotype enzymology genetics Lotus (genus) metabolism oxidative stress photosynthesis Adaptation, Biological Antioxidants Cold Temperature Ecotype Loteae Oxidative Stress Photosynthesis Reactive Oxygen Species Calzadilla, Pablo Ignacio Menéndez, Ana Bernardina Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature
topic_facet	Chilling D2 protein Legumes Photoinhibition PSII ROS antioxidant reactive oxygen metabolite adaptation cold ecotype enzymology genetics Lotus (genus) metabolism oxidative stress photosynthesis Adaptation, Biological Antioxidants Cold Temperature Ecotype Loteae Oxidative Stress Photosynthesis Reactive Oxygen Species
description	Lotus species are important forage legumes due to their high nutritional value and adaptability to marginal conditions. However, the dry matter production and regrowth rate of cultivable Lotus spp. is drastically reduced during colder seasons. In this work, we evaluated the chilling response of Lotus japonicus ecotypes MG-1 and MG-20. No significant increases were observed in reactive oxygen species and nitric oxide production or in lipid peroxidation, although a chilling-induced redox imbalance was suggested through NADPH/NADP+ ratio alterations. Antioxidant enzyme catalase, ascorbate peroxidase, and superoxide dismutase activities were also measured. Superoxide dismutase, in particular the chloroplastic isoform, showed different activity for different ecotypes and treatments. Stress-induced photoinhibition also differentially influenced both ecotypes, with MG-1 more affected than MG-20. Data showed that the D2 PSII subunit was more affected than D1 after 1 d of low temperature exposure, although its protein levels recovered over the course of the experiment. Interestingly, D2 recovery was accompanied by improvements in photosynthetic parameters (Asat and Fv/Fm) and the NADPH/NADP+ ratio. Our results suggest that the D2 protein is involved in the acclimation response of L. japonicus to low temperature. This may provide a deeper insight into the chilling tolerance mechanisms of the Lotus genus. © 2016 Elsevier Ireland Ltd.
author	Calzadilla, Pablo Ignacio Menéndez, Ana Bernardina
author_facet	Calzadilla, Pablo Ignacio Menéndez, Ana Bernardina
author_sort	Calzadilla, Pablo Ignacio
title	Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature
title_short	Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature
title_full	Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature
title_fullStr	Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature
title_full_unstemmed	Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature
title_sort	photosynthetic responses mediate the adaptation of two lotus japonicus ecotypes to low temperature
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v250_n_p59_Calzadilla http://hdl.handle.net/20.500.12110/paper_01689452_v250_n_p59_Calzadilla
work_keys_str_mv	AT calzadillapabloignacio photosyntheticresponsesmediatetheadaptationoftwolotusjaponicusecotypestolowtemperature AT menendezanabernardina photosyntheticresponsesmediatetheadaptationoftwolotusjaponicusecotypestolowtemperature
_version_	1768545876378124288

Photosynthetic responses mediate the adaptation of two Lotus japonicus ecotypes to low temperature

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