Cellular and molecular aspects of quinoa leaf senescence

During leaf senescence, degradation of chloroplasts precede to changes in nuclei and other cytoplasmic organelles, RuBisCO stability is progressively lost, grana lose their structure, plastidial DNA becomes distorted and degraded, the number of plastoglobuli increases and abundant senescence-associa...

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Autor principal:	Martínez Tosar, Leandro Julián
Publicado:	2015
Materias:	DNA fragmentation Endoreduplication Leaf senescence Nucleases Quinoa Chenopodium quinoa deoxyribonuclease ribulosebisphosphate carboxylase cell nucleus chloroplast cytology flow cytometry genetics growth, development and aging metabolism plant leaf ploidy ultrastructure Cell Nucleus Chloroplasts Deoxyribonucleases DNA Fragmentation Flow Cytometry Plant Leaves Ploidies Ribulose-Bisphosphate Carboxylase
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v238_n_p178_LopezFernandez http://hdl.handle.net/20.500.12110/paper_01689452_v238_n_p178_LopezFernandez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01689452_v238_n_p178_LopezFernandez
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spelling	paper:paper_01689452_v238_n_p178_LopezFernandez2023-06-08T15:18:08Z Cellular and molecular aspects of quinoa leaf senescence Martínez Tosar, Leandro Julián DNA fragmentation Endoreduplication Leaf senescence Nucleases Quinoa Chenopodium quinoa deoxyribonuclease ribulosebisphosphate carboxylase cell nucleus Chenopodium quinoa chloroplast cytology DNA fragmentation flow cytometry genetics growth, development and aging metabolism plant leaf ploidy ultrastructure Cell Nucleus Chenopodium quinoa Chloroplasts Deoxyribonucleases DNA Fragmentation Flow Cytometry Plant Leaves Ploidies Ribulose-Bisphosphate Carboxylase During leaf senescence, degradation of chloroplasts precede to changes in nuclei and other cytoplasmic organelles, RuBisCO stability is progressively lost, grana lose their structure, plastidial DNA becomes distorted and degraded, the number of plastoglobuli increases and abundant senescence-associated vesicles containing electronically dense particles emerge from chloroplasts pouring their content into the central vacuole. This study examines quinoa leaf tissues during development and senescence using a range of well-established markers of programmed cell death (PCD), including: morphological changes in nuclei and chloroplasts, degradation of RuBisCO, changes in chlorophyll content, DNA degradation, variations in ploidy levels, and changes in nuclease profiles. TUNEL reaction and DNA electrophoresis demonstrated that DNA fragmentation in nuclei occurs at early senescence, which correlates with induction of specific nucleases. During senescence, metabolic activity is high and nuclei endoreduplicate, peaking at 4. C. At this time, TEM images showed some healthy nuclei with condensed chromatin and nucleoli. We have found that DNA fragmentation, induction of senescence-associated nucleases and endoreduplication take place during leaf senescence. This provides a starting point for further research aiming to identify key genes involved in the senescence of quinoa leaves. © 2015 . Fil:Martínez-Tosar, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v238_n_p178_LopezFernandez http://hdl.handle.net/20.500.12110/paper_01689452_v238_n_p178_LopezFernandez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	DNA fragmentation Endoreduplication Leaf senescence Nucleases Quinoa Chenopodium quinoa deoxyribonuclease ribulosebisphosphate carboxylase cell nucleus Chenopodium quinoa chloroplast cytology DNA fragmentation flow cytometry genetics growth, development and aging metabolism plant leaf ploidy ultrastructure Cell Nucleus Chenopodium quinoa Chloroplasts Deoxyribonucleases DNA Fragmentation Flow Cytometry Plant Leaves Ploidies Ribulose-Bisphosphate Carboxylase
spellingShingle	DNA fragmentation Endoreduplication Leaf senescence Nucleases Quinoa Chenopodium quinoa deoxyribonuclease ribulosebisphosphate carboxylase cell nucleus Chenopodium quinoa chloroplast cytology DNA fragmentation flow cytometry genetics growth, development and aging metabolism plant leaf ploidy ultrastructure Cell Nucleus Chenopodium quinoa Chloroplasts Deoxyribonucleases DNA Fragmentation Flow Cytometry Plant Leaves Ploidies Ribulose-Bisphosphate Carboxylase Martínez Tosar, Leandro Julián Cellular and molecular aspects of quinoa leaf senescence
topic_facet	DNA fragmentation Endoreduplication Leaf senescence Nucleases Quinoa Chenopodium quinoa deoxyribonuclease ribulosebisphosphate carboxylase cell nucleus Chenopodium quinoa chloroplast cytology DNA fragmentation flow cytometry genetics growth, development and aging metabolism plant leaf ploidy ultrastructure Cell Nucleus Chenopodium quinoa Chloroplasts Deoxyribonucleases DNA Fragmentation Flow Cytometry Plant Leaves Ploidies Ribulose-Bisphosphate Carboxylase
description	During leaf senescence, degradation of chloroplasts precede to changes in nuclei and other cytoplasmic organelles, RuBisCO stability is progressively lost, grana lose their structure, plastidial DNA becomes distorted and degraded, the number of plastoglobuli increases and abundant senescence-associated vesicles containing electronically dense particles emerge from chloroplasts pouring their content into the central vacuole. This study examines quinoa leaf tissues during development and senescence using a range of well-established markers of programmed cell death (PCD), including: morphological changes in nuclei and chloroplasts, degradation of RuBisCO, changes in chlorophyll content, DNA degradation, variations in ploidy levels, and changes in nuclease profiles. TUNEL reaction and DNA electrophoresis demonstrated that DNA fragmentation in nuclei occurs at early senescence, which correlates with induction of specific nucleases. During senescence, metabolic activity is high and nuclei endoreduplicate, peaking at 4. C. At this time, TEM images showed some healthy nuclei with condensed chromatin and nucleoli. We have found that DNA fragmentation, induction of senescence-associated nucleases and endoreduplication take place during leaf senescence. This provides a starting point for further research aiming to identify key genes involved in the senescence of quinoa leaves. © 2015 .
author	Martínez Tosar, Leandro Julián
author_facet	Martínez Tosar, Leandro Julián
author_sort	Martínez Tosar, Leandro Julián
title	Cellular and molecular aspects of quinoa leaf senescence
title_short	Cellular and molecular aspects of quinoa leaf senescence
title_full	Cellular and molecular aspects of quinoa leaf senescence
title_fullStr	Cellular and molecular aspects of quinoa leaf senescence
title_full_unstemmed	Cellular and molecular aspects of quinoa leaf senescence
title_sort	cellular and molecular aspects of quinoa leaf senescence
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01689452_v238_n_p178_LopezFernandez http://hdl.handle.net/20.500.12110/paper_01689452_v238_n_p178_LopezFernandez
work_keys_str_mv	AT martineztosarleandrojulian cellularandmolecularaspectsofquinoaleafsenescence
_version_	1768543226026786816

Cellular and molecular aspects of quinoa leaf senescence

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