Microbial community composition along a 50 000-year lacustrine sediment sequence
For decades, microbial community composition in subseafloor sediments has been the focus of extensive studies. In deep lacustrine sediments, however, the taxonomic composition of microbial communities remains undercharacterized. Greater knowledge on microbial diversity in lacustrine sediments would...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01686496_v94_n4_p_Vuillemin http://hdl.handle.net/20.500.12110/paper_01686496_v94_n4_p_Vuillemin |
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paper:paper_01686496_v94_n4_p_Vuillemin2023-06-08T15:17:57Z Microbial community composition along a 50 000-year lacustrine sediment sequence 16S rRNA gene high-throughput sequencing Deep biosphere Laguna Potrok Aike Lake sediment Microbial communities Species selection bacterium biosphere community composition environmental factor Holocene lacustrine deposit Last Glacial-Interglacial marine sediment microbial community paleoenvironment prokaryote seafloor sedimentary sequence taxonomy Argentina Laguna Potrok Aike Santa Cruz [Argentina] RNA 16S aquatic species archaeon Argentina bacterium classification genetics high throughput sequencing microbiology microflora phylogeny salinity sediment Aquatic Organisms Archaea Argentina Bacteria Geologic Sediments High-Throughput Nucleotide Sequencing Microbiota Phylogeny RNA, Ribosomal, 16S Salinity For decades, microbial community composition in subseafloor sediments has been the focus of extensive studies. In deep lacustrine sediments, however, the taxonomic composition of microbial communities remains undercharacterized. Greater knowledge on microbial diversity in lacustrine sediments would improve our understanding of how environmental factors, and resulting selective pressures, shape subsurface biospheres in marine and freshwater sediments. Using high-throughput sequencing of 16S rRNA genes across high-resolution climate intervals covering the last 50 000 years in Laguna Potrok Aike, Argentina, we identified changes in microbial populations in response to both past environmental conditions and geochemical changes of the sediment during burial. Microbial communities in Holocene sediments were most diverse, reflecting a layering of taxa linked to electron acceptors availability. In deeper intervals, the data show that salinity, organic matter and the depositional conditions over the Last Glacial-interglacial cycle were all selective pressures in the deep lacustrine assemblage resulting in a genetically distinct biosphere from the surface dominated primarily by Bathyarchaeota and Atribacteria groups. However, similar to marine sediments, some dominant taxa in the shallow subsurface persisted into the subsurface as minor fraction of the community. The subsequent establishment of a deep subsurface community likely results from a combination of paleoenvironmental factors that have shaped the pool of available substrates, together with substrate depletion and/or reworking of organic matter with depth. © FEMS 2018. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01686496_v94_n4_p_Vuillemin http://hdl.handle.net/20.500.12110/paper_01686496_v94_n4_p_Vuillemin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
16S rRNA gene high-throughput sequencing Deep biosphere Laguna Potrok Aike Lake sediment Microbial communities Species selection bacterium biosphere community composition environmental factor Holocene lacustrine deposit Last Glacial-Interglacial marine sediment microbial community paleoenvironment prokaryote seafloor sedimentary sequence taxonomy Argentina Laguna Potrok Aike Santa Cruz [Argentina] RNA 16S aquatic species archaeon Argentina bacterium classification genetics high throughput sequencing microbiology microflora phylogeny salinity sediment Aquatic Organisms Archaea Argentina Bacteria Geologic Sediments High-Throughput Nucleotide Sequencing Microbiota Phylogeny RNA, Ribosomal, 16S Salinity |
| spellingShingle |
16S rRNA gene high-throughput sequencing Deep biosphere Laguna Potrok Aike Lake sediment Microbial communities Species selection bacterium biosphere community composition environmental factor Holocene lacustrine deposit Last Glacial-Interglacial marine sediment microbial community paleoenvironment prokaryote seafloor sedimentary sequence taxonomy Argentina Laguna Potrok Aike Santa Cruz [Argentina] RNA 16S aquatic species archaeon Argentina bacterium classification genetics high throughput sequencing microbiology microflora phylogeny salinity sediment Aquatic Organisms Archaea Argentina Bacteria Geologic Sediments High-Throughput Nucleotide Sequencing Microbiota Phylogeny RNA, Ribosomal, 16S Salinity Microbial community composition along a 50 000-year lacustrine sediment sequence |
| topic_facet |
16S rRNA gene high-throughput sequencing Deep biosphere Laguna Potrok Aike Lake sediment Microbial communities Species selection bacterium biosphere community composition environmental factor Holocene lacustrine deposit Last Glacial-Interglacial marine sediment microbial community paleoenvironment prokaryote seafloor sedimentary sequence taxonomy Argentina Laguna Potrok Aike Santa Cruz [Argentina] RNA 16S aquatic species archaeon Argentina bacterium classification genetics high throughput sequencing microbiology microflora phylogeny salinity sediment Aquatic Organisms Archaea Argentina Bacteria Geologic Sediments High-Throughput Nucleotide Sequencing Microbiota Phylogeny RNA, Ribosomal, 16S Salinity |
| description |
For decades, microbial community composition in subseafloor sediments has been the focus of extensive studies. In deep lacustrine sediments, however, the taxonomic composition of microbial communities remains undercharacterized. Greater knowledge on microbial diversity in lacustrine sediments would improve our understanding of how environmental factors, and resulting selective pressures, shape subsurface biospheres in marine and freshwater sediments. Using high-throughput sequencing of 16S rRNA genes across high-resolution climate intervals covering the last 50 000 years in Laguna Potrok Aike, Argentina, we identified changes in microbial populations in response to both past environmental conditions and geochemical changes of the sediment during burial. Microbial communities in Holocene sediments were most diverse, reflecting a layering of taxa linked to electron acceptors availability. In deeper intervals, the data show that salinity, organic matter and the depositional conditions over the Last Glacial-interglacial cycle were all selective pressures in the deep lacustrine assemblage resulting in a genetically distinct biosphere from the surface dominated primarily by Bathyarchaeota and Atribacteria groups. However, similar to marine sediments, some dominant taxa in the shallow subsurface persisted into the subsurface as minor fraction of the community. The subsequent establishment of a deep subsurface community likely results from a combination of paleoenvironmental factors that have shaped the pool of available substrates, together with substrate depletion and/or reworking of organic matter with depth. © FEMS 2018. |
| title |
Microbial community composition along a 50 000-year lacustrine sediment sequence |
| title_short |
Microbial community composition along a 50 000-year lacustrine sediment sequence |
| title_full |
Microbial community composition along a 50 000-year lacustrine sediment sequence |
| title_fullStr |
Microbial community composition along a 50 000-year lacustrine sediment sequence |
| title_full_unstemmed |
Microbial community composition along a 50 000-year lacustrine sediment sequence |
| title_sort |
microbial community composition along a 50 000-year lacustrine sediment sequence |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01686496_v94_n4_p_Vuillemin http://hdl.handle.net/20.500.12110/paper_01686496_v94_n4_p_Vuillemin |
| _version_ |
1768544129098186752 |