The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are cellular factors involved in the replication of several viruses. In this study we analyzed the expression and intracellular localization of hnRNP A2 and hnRNP K in cell cultures infected with two viruses that cause human hemorrhagic fevers: dengu...
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paper:paper_01681702_v203_n_p84_Brunetti2023-06-08T15:17:40Z The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication Scolaro, Luis Alberto Castilla, Viviana Dengue virus Heterogeneous nuclear ribonucleoproteins HnRNP A2 HnRNP K Junín virus heterogeneous nuclear ribonucleoprotein heterogeneous nuclear ribonucleoprotein A2 heterogeneous nuclear ribonucleoprotein K host factor unclassified drug virus protein heterogeneous nuclear ribonucleoprotein K hnRNP A2 A549 cell line animal cell Article cell culture controlled study cytoplasm Dengue virus 2 genetic transfection human human cell immunofluorescence test Junin virus nonhuman persistent infection plasmid priority journal protein expression protein localization superinfection supernatant Vero cell line virogenesis Western blotting animal cell line cell nucleus chemistry Dengue virus host pathogen interaction Junin virus metabolism physiology virus replication Dengue virus Dengue virus type 2 Animals Cell Line Cell Nucleus Cytoplasm Dengue Virus Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoprotein K Host-Pathogen Interactions Humans Junin virus Virus Replication Heterogeneous nuclear ribonucleoproteins (hnRNPs) are cellular factors involved in the replication of several viruses. In this study we analyzed the expression and intracellular localization of hnRNP A2 and hnRNP K in cell cultures infected with two viruses that cause human hemorrhagic fevers: dengue virus type 2 (DENV-2) and Junín virus (JUNV). We determined that DENV-2 promoted the cytoplasmic translocation of hnRNP K and to a lesser extent of hnRNP A2, meanwhile, JUNV infection induced an increase in hnRNP K cytoplasmic localization whereas hnRNP A2 remained mainly in the nucleus of infected cells. Both hnRNP K and hnRNP A2 were localized predominantly in the nucleus of JUNV persistently-infected cells even after superinfection with JUNV indicating that persistent infection does not alter nucleo-cytoplasmic transport of these hnRNPs. Total levels of hnRNP K expression were unaffected by DENV-2 or JUNV infection. In addition we determined, using small interfering RNAs, that hnRNP K knockout inhibits DENV-2 and JUNV multiplication. Our results indicate that DENV-2 and JUNV induce hnRNP K cytoplasmic translocation to favor viral multiplication. © 2015 Elsevier B.V. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v203_n_p84_Brunetti http://hdl.handle.net/20.500.12110/paper_01681702_v203_n_p84_Brunetti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Dengue virus Heterogeneous nuclear ribonucleoproteins HnRNP A2 HnRNP K Junín virus heterogeneous nuclear ribonucleoprotein heterogeneous nuclear ribonucleoprotein A2 heterogeneous nuclear ribonucleoprotein K host factor unclassified drug virus protein heterogeneous nuclear ribonucleoprotein K hnRNP A2 A549 cell line animal cell Article cell culture controlled study cytoplasm Dengue virus 2 genetic transfection human human cell immunofluorescence test Junin virus nonhuman persistent infection plasmid priority journal protein expression protein localization superinfection supernatant Vero cell line virogenesis Western blotting animal cell line cell nucleus chemistry Dengue virus host pathogen interaction Junin virus metabolism physiology virus replication Dengue virus Dengue virus type 2 Animals Cell Line Cell Nucleus Cytoplasm Dengue Virus Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoprotein K Host-Pathogen Interactions Humans Junin virus Virus Replication |
| spellingShingle |
Dengue virus Heterogeneous nuclear ribonucleoproteins HnRNP A2 HnRNP K Junín virus heterogeneous nuclear ribonucleoprotein heterogeneous nuclear ribonucleoprotein A2 heterogeneous nuclear ribonucleoprotein K host factor unclassified drug virus protein heterogeneous nuclear ribonucleoprotein K hnRNP A2 A549 cell line animal cell Article cell culture controlled study cytoplasm Dengue virus 2 genetic transfection human human cell immunofluorescence test Junin virus nonhuman persistent infection plasmid priority journal protein expression protein localization superinfection supernatant Vero cell line virogenesis Western blotting animal cell line cell nucleus chemistry Dengue virus host pathogen interaction Junin virus metabolism physiology virus replication Dengue virus Dengue virus type 2 Animals Cell Line Cell Nucleus Cytoplasm Dengue Virus Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoprotein K Host-Pathogen Interactions Humans Junin virus Virus Replication Scolaro, Luis Alberto Castilla, Viviana The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication |
| topic_facet |
Dengue virus Heterogeneous nuclear ribonucleoproteins HnRNP A2 HnRNP K Junín virus heterogeneous nuclear ribonucleoprotein heterogeneous nuclear ribonucleoprotein A2 heterogeneous nuclear ribonucleoprotein K host factor unclassified drug virus protein heterogeneous nuclear ribonucleoprotein K hnRNP A2 A549 cell line animal cell Article cell culture controlled study cytoplasm Dengue virus 2 genetic transfection human human cell immunofluorescence test Junin virus nonhuman persistent infection plasmid priority journal protein expression protein localization superinfection supernatant Vero cell line virogenesis Western blotting animal cell line cell nucleus chemistry Dengue virus host pathogen interaction Junin virus metabolism physiology virus replication Dengue virus Dengue virus type 2 Animals Cell Line Cell Nucleus Cytoplasm Dengue Virus Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoprotein K Host-Pathogen Interactions Humans Junin virus Virus Replication |
| description |
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are cellular factors involved in the replication of several viruses. In this study we analyzed the expression and intracellular localization of hnRNP A2 and hnRNP K in cell cultures infected with two viruses that cause human hemorrhagic fevers: dengue virus type 2 (DENV-2) and Junín virus (JUNV). We determined that DENV-2 promoted the cytoplasmic translocation of hnRNP K and to a lesser extent of hnRNP A2, meanwhile, JUNV infection induced an increase in hnRNP K cytoplasmic localization whereas hnRNP A2 remained mainly in the nucleus of infected cells. Both hnRNP K and hnRNP A2 were localized predominantly in the nucleus of JUNV persistently-infected cells even after superinfection with JUNV indicating that persistent infection does not alter nucleo-cytoplasmic transport of these hnRNPs. Total levels of hnRNP K expression were unaffected by DENV-2 or JUNV infection. In addition we determined, using small interfering RNAs, that hnRNP K knockout inhibits DENV-2 and JUNV multiplication. Our results indicate that DENV-2 and JUNV induce hnRNP K cytoplasmic translocation to favor viral multiplication. © 2015 Elsevier B.V. |
| author |
Scolaro, Luis Alberto Castilla, Viviana |
| author_facet |
Scolaro, Luis Alberto Castilla, Viviana |
| author_sort |
Scolaro, Luis Alberto |
| title |
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication |
| title_short |
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication |
| title_full |
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication |
| title_fullStr |
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication |
| title_full_unstemmed |
The heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a host factor required for dengue virus and Junín virus multiplication |
| title_sort |
heterogeneous nuclear ribonucleoprotein k (hnrnp k) is a host factor required for dengue virus and junín virus multiplication |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v203_n_p84_Brunetti http://hdl.handle.net/20.500.12110/paper_01681702_v203_n_p84_Brunetti |
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AT scolaroluisalberto theheterogeneousnuclearribonucleoproteinkhnrnpkisahostfactorrequiredfordenguevirusandjuninvirusmultiplication AT castillaviviana theheterogeneousnuclearribonucleoproteinkhnrnpkisahostfactorrequiredfordenguevirusandjuninvirusmultiplication AT scolaroluisalberto heterogeneousnuclearribonucleoproteinkhnrnpkisahostfactorrequiredfordenguevirusandjuninvirusmultiplication AT castillaviviana heterogeneousnuclearribonucleoproteinkhnrnpkisahostfactorrequiredfordenguevirusandjuninvirusmultiplication |
| _version_ |
1768546110688722944 |