The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing
Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated t...
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2014
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v180_n_p70_Delfosse http://hdl.handle.net/20.500.12110/paper_01681702_v180_n_p70_Delfosse |
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paper:paper_01681702_v180_n_p70_Delfosse2023-06-08T15:17:39Z The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing Hopp, Horacio Esteban Distéfano, Ana Julia CLRDV P0 protein Polerovirus Silencing suppressor P0 protein RNA small interfering RNA unclassified drug virus protein Agrobacterium article cotton leafroll dwarf virus enzyme inhibition inoculation inverted repeat molecular cloning Nicotiana benthamiana nonhuman Northern blotting Polerovirus priority journal transgene virus genome CLRDV P0 protein Polerovirus Silencing suppressor Agrobacterium Host-Pathogen Interactions Luteoviridae RNA Interference Tobacco Transgenes Viral Proteins Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamiana plants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana through Agrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggesting that the P0 protein exhibits similar RNA silencing suppression activity when expressed from the full-length viral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemic silencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation of secondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contribute to understanding the molecular mechanisms involved in the induction of cotton blue disease by CLRDV infection. © 2013 Elsevier B.V. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v180_n_p70_Delfosse http://hdl.handle.net/20.500.12110/paper_01681702_v180_n_p70_Delfosse |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CLRDV P0 protein Polerovirus Silencing suppressor P0 protein RNA small interfering RNA unclassified drug virus protein Agrobacterium article cotton leafroll dwarf virus enzyme inhibition inoculation inverted repeat molecular cloning Nicotiana benthamiana nonhuman Northern blotting Polerovirus priority journal transgene virus genome CLRDV P0 protein Polerovirus Silencing suppressor Agrobacterium Host-Pathogen Interactions Luteoviridae RNA Interference Tobacco Transgenes Viral Proteins |
| spellingShingle |
CLRDV P0 protein Polerovirus Silencing suppressor P0 protein RNA small interfering RNA unclassified drug virus protein Agrobacterium article cotton leafroll dwarf virus enzyme inhibition inoculation inverted repeat molecular cloning Nicotiana benthamiana nonhuman Northern blotting Polerovirus priority journal transgene virus genome CLRDV P0 protein Polerovirus Silencing suppressor Agrobacterium Host-Pathogen Interactions Luteoviridae RNA Interference Tobacco Transgenes Viral Proteins Hopp, Horacio Esteban Distéfano, Ana Julia The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| topic_facet |
CLRDV P0 protein Polerovirus Silencing suppressor P0 protein RNA small interfering RNA unclassified drug virus protein Agrobacterium article cotton leafroll dwarf virus enzyme inhibition inoculation inverted repeat molecular cloning Nicotiana benthamiana nonhuman Northern blotting Polerovirus priority journal transgene virus genome CLRDV P0 protein Polerovirus Silencing suppressor Agrobacterium Host-Pathogen Interactions Luteoviridae RNA Interference Tobacco Transgenes Viral Proteins |
| description |
Plants employ RNA silencing as a natural defense mechanism against viruses. As a counter-defense, viruses encode silencing suppressor proteins (SSPs) that suppress RNA silencing. Most, but not all, the P0 proteins encoded by poleroviruses have been identified as SSP. In this study, we demonstrated that cotton leafroll dwarf virus (CLRDV, genus Polerovirus) P0 protein suppressed local silencing that was induced by sense or inverted repeat transgenes in Agrobacterium co-infiltration assay in Nicotiana benthamiana plants. A CLRDV full-length infectious cDNA clone that is able to infect N. benthamiana through Agrobacterium-mediated inoculation also inhibited local silencing in co-infiltration assays, suggesting that the P0 protein exhibits similar RNA silencing suppression activity when expressed from the full-length viral genome. On the other hand, the P0 protein did not efficiently inhibit the spread of systemic silencing signals. Moreover, Northern blotting indicated that the P0 protein inhibits the generation of secondary but not primary small interfering RNAs. The study of CLRDV P0 suppression activity may contribute to understanding the molecular mechanisms involved in the induction of cotton blue disease by CLRDV infection. © 2013 Elsevier B.V. |
| author |
Hopp, Horacio Esteban Distéfano, Ana Julia |
| author_facet |
Hopp, Horacio Esteban Distéfano, Ana Julia |
| author_sort |
Hopp, Horacio Esteban |
| title |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_short |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_full |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_fullStr |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_full_unstemmed |
The P0 protein encoded by cotton leafroll dwarf virus (CLRDV) inhibits local but not systemic RNA silencing |
| title_sort |
p0 protein encoded by cotton leafroll dwarf virus (clrdv) inhibits local but not systemic rna silencing |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v180_n_p70_Delfosse http://hdl.handle.net/20.500.12110/paper_01681702_v180_n_p70_Delfosse |
| work_keys_str_mv |
AT hopphoracioesteban thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT distefanoanajulia thep0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT hopphoracioesteban p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing AT distefanoanajulia p0proteinencodedbycottonleafrolldwarfvirusclrdvinhibitslocalbutnotsystemicrnasilencing |
| _version_ |
1768542642119901184 |