Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts
Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed v...
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paper:paper_01681702_v152_n1-2_p96_Maroniche2023-06-08T15:17:38Z Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Distéfano, Ana Julia Taboga, Oscar A. Hopp, Horacio Esteban Del Vas, Mariana ATPase Fijivirus Inclusion body MRCV Plant reovirus Self-interaction SsRNA binding Viroplasm adenosine triphosphatase single stranded RNA virus protein article bacterium controlled study enzyme activity Lepidoptera Mal de Rio Cuarto virus molecular weight nonhuman nucleotide sequence open reading frame priority journal Reovirus virus inclusion yeast animal chemistry genetics metabolism protein motif Reoviridae Spodoptera virology Amino Acid Motifs Animals Inclusion Bodies, Viral Open Reading Frames Reoviridae Spodoptera Viral Proteins Animalia Archaeorrhyncha Fijivirus Hexapoda Mal de Rio Cuarto virus Orthoreovirus Reoviridae Spodoptera frugiperda Amino Acid Motifs Animals Inclusion Bodies, Viral Open Reading Frames Reoviridae Spodoptera Viral Proteins Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed viroplasms that are formed in the cytoplasm of infected plant and insect cells. In this study, the protein coded by the first ORF of MRCV segment S9 (P9-1) was shown to establish cytoplasmic inclusion bodies resembling viroplasms after transfection of Spodoptera frugiperda insect cells. In accordance, MRCV P9-1 self-associates giving rise to high molecular weight complexes when expressed in bacteria. Strong self-interaction was also evidenced by yeast two-hybrid assays. Furthermore, biochemical characterization showed that MRCV P9-1 bound single stranded RNA and had ATPase activity. Finally, the MRCV P9-1 region required for the formation of VIB-like structures was mapped to the protein carboxy-terminal half. This extensive functional and biochemical characterization of MRCV P9-1 revealed further similarities between plant and animal reovirus viroplasm proteins. © 2010 Elsevier B.V. Fil:Maroniche, G.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mongelli, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Peralta, A.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Distéfano, A.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Taboga, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Vas, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v152_n1-2_p96_Maroniche http://hdl.handle.net/20.500.12110/paper_01681702_v152_n1-2_p96_Maroniche |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
ATPase Fijivirus Inclusion body MRCV Plant reovirus Self-interaction SsRNA binding Viroplasm adenosine triphosphatase single stranded RNA virus protein article bacterium controlled study enzyme activity Lepidoptera Mal de Rio Cuarto virus molecular weight nonhuman nucleotide sequence open reading frame priority journal Reovirus virus inclusion yeast animal chemistry genetics metabolism protein motif Reoviridae Spodoptera virology Amino Acid Motifs Animals Inclusion Bodies, Viral Open Reading Frames Reoviridae Spodoptera Viral Proteins Animalia Archaeorrhyncha Fijivirus Hexapoda Mal de Rio Cuarto virus Orthoreovirus Reoviridae Spodoptera frugiperda Amino Acid Motifs Animals Inclusion Bodies, Viral Open Reading Frames Reoviridae Spodoptera Viral Proteins |
spellingShingle |
ATPase Fijivirus Inclusion body MRCV Plant reovirus Self-interaction SsRNA binding Viroplasm adenosine triphosphatase single stranded RNA virus protein article bacterium controlled study enzyme activity Lepidoptera Mal de Rio Cuarto virus molecular weight nonhuman nucleotide sequence open reading frame priority journal Reovirus virus inclusion yeast animal chemistry genetics metabolism protein motif Reoviridae Spodoptera virology Amino Acid Motifs Animals Inclusion Bodies, Viral Open Reading Frames Reoviridae Spodoptera Viral Proteins Animalia Archaeorrhyncha Fijivirus Hexapoda Mal de Rio Cuarto virus Orthoreovirus Reoviridae Spodoptera frugiperda Amino Acid Motifs Animals Inclusion Bodies, Viral Open Reading Frames Reoviridae Spodoptera Viral Proteins Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Distéfano, Ana Julia Taboga, Oscar A. Hopp, Horacio Esteban Del Vas, Mariana Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
topic_facet |
ATPase Fijivirus Inclusion body MRCV Plant reovirus Self-interaction SsRNA binding Viroplasm adenosine triphosphatase single stranded RNA virus protein article bacterium controlled study enzyme activity Lepidoptera Mal de Rio Cuarto virus molecular weight nonhuman nucleotide sequence open reading frame priority journal Reovirus virus inclusion yeast animal chemistry genetics metabolism protein motif Reoviridae Spodoptera virology Amino Acid Motifs Animals Inclusion Bodies, Viral Open Reading Frames Reoviridae Spodoptera Viral Proteins Animalia Archaeorrhyncha Fijivirus Hexapoda Mal de Rio Cuarto virus Orthoreovirus Reoviridae Spodoptera frugiperda Amino Acid Motifs Animals Inclusion Bodies, Viral Open Reading Frames Reoviridae Spodoptera Viral Proteins |
description |
Mal de Río Cuarto virus (MRCV) is a plant virus of the genus Fijivirus within the family Reoviridae that infects several monocotyledonous species and is transmitted by planthoppers in a persistent and propagative manner. Other members of the family replicate in viral inclusion bodies (VIBs) termed viroplasms that are formed in the cytoplasm of infected plant and insect cells. In this study, the protein coded by the first ORF of MRCV segment S9 (P9-1) was shown to establish cytoplasmic inclusion bodies resembling viroplasms after transfection of Spodoptera frugiperda insect cells. In accordance, MRCV P9-1 self-associates giving rise to high molecular weight complexes when expressed in bacteria. Strong self-interaction was also evidenced by yeast two-hybrid assays. Furthermore, biochemical characterization showed that MRCV P9-1 bound single stranded RNA and had ATPase activity. Finally, the MRCV P9-1 region required for the formation of VIB-like structures was mapped to the protein carboxy-terminal half. This extensive functional and biochemical characterization of MRCV P9-1 revealed further similarities between plant and animal reovirus viroplasm proteins. © 2010 Elsevier B.V. |
author |
Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Distéfano, Ana Julia Taboga, Oscar A. Hopp, Horacio Esteban Del Vas, Mariana |
author_facet |
Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Peralta, Andrea Distéfano, Ana Julia Taboga, Oscar A. Hopp, Horacio Esteban Del Vas, Mariana |
author_sort |
Maroniche, Guillermo Andrés |
title |
Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
title_short |
Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
title_full |
Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
title_fullStr |
Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
title_full_unstemmed |
Functional and biochemical properties of Mal de Río Cuarto virus (Fijivirus, Reoviridae) P9-1 viroplasm protein show further similarities to animal reovirus counterparts |
title_sort |
functional and biochemical properties of mal de río cuarto virus (fijivirus, reoviridae) p9-1 viroplasm protein show further similarities to animal reovirus counterparts |
publishDate |
2010 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v152_n1-2_p96_Maroniche http://hdl.handle.net/20.500.12110/paper_01681702_v152_n1-2_p96_Maroniche |
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