Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro

In this paper we demonstrate that infection of cell cultures with the arenavirus Junín (JUNV), agent of the argentine haemorrhagic fever, leads to the activation of PI3K/Akt signalling pathway. Phosphorylation of Akt occurs early during JUNV infection of Vero cells and is blocked by the PI3K inhibit...

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Autores principales:	Linero, Florencia N., Scolaro, Luis Alberto
Publicado:	2009
Materias:	Akt Argentine hemorrhagic fever Junín virus Ly294002 Phosphatidylinositol 3-kinase cell receptor phosphatidylinositol 3 kinase protein kinase B transferrin receptor animal cell article cell surface controlled study enzyme activation enzyme inhibition enzyme phosphorylation human human cell in vitro study Junin virus nonhuman persistent virus infection priority journal protein expression protein synthesis ultraviolet radiation Vero cell virus activation virus cell interaction virus entry virus infection virus replication 1-Phosphatidylinositol 3-Kinase Animals Cercopithecus aethiops Chromones Cricetinae Enzyme Inhibitors Hemorrhagic Fever, American Host-Pathogen Interactions Humans Morpholines Phosphorylation Proto-Oncogene Proteins c-akt Signal Transduction Vero Cells Virus Internalization Virus Replication Arenavirus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v145_n1_p166_Linero http://hdl.handle.net/20.500.12110/paper_01681702_v145_n1_p166_Linero
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01681702_v145_n1_p166_Linero
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spelling	paper:paper_01681702_v145_n1_p166_Linero2023-06-08T15:17:37Z Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro Linero, Florencia N. Scolaro, Luis Alberto Akt Argentine hemorrhagic fever Junín virus Ly294002 Phosphatidylinositol 3-kinase cell receptor phosphatidylinositol 3 kinase protein kinase B transferrin receptor animal cell article cell surface controlled study enzyme activation enzyme inhibition enzyme phosphorylation human human cell in vitro study Junin virus nonhuman persistent virus infection priority journal protein expression protein synthesis ultraviolet radiation Vero cell virus activation virus cell interaction virus entry virus infection virus replication 1-Phosphatidylinositol 3-Kinase Animals Cercopithecus aethiops Chromones Cricetinae Enzyme Inhibitors Hemorrhagic Fever, American Host-Pathogen Interactions Humans Junin virus Morpholines Phosphorylation Proto-Oncogene Proteins c-akt Signal Transduction Vero Cells Virus Internalization Virus Replication Arenavirus Junin virus In this paper we demonstrate that infection of cell cultures with the arenavirus Junín (JUNV), agent of the argentine haemorrhagic fever, leads to the activation of PI3K/Akt signalling pathway. Phosphorylation of Akt occurs early during JUNV infection of Vero cells and is blocked by the PI3K inhibitor, Ly294002. Infection of cells with UV-irradiated JUNV redeemed the pattern of stimulation observed for infectious virus indicating that an early stage of multiplication cycle would be enough to trigger activation. Treatment of cells with chlorpromazine abrogated phosphorylation of Akt upon JUNV infection suggesting virus internalization as responsible for activation. Inhibition of Akt phosphorylation by Ly294002 impaired viral protein synthesis and expression leading to a reduced infectious virus yield without blocking the onset of persistent stage of infection. This impairment is linked to a reduced amount of virus bound to cells probably due to a blockage on the recycling of transferrin cell-receptor, employed by the virus to adsorb to the cell surface. Early Akt activation was also observed in BHK-21 and A549 JUNV infected cells suggesting an important role of PI3K/Akt signalling in JUNV multiplication in vitro. © 2009 Elsevier B.V. All rights reserved. Fil:Linero, F.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v145_n1_p166_Linero http://hdl.handle.net/20.500.12110/paper_01681702_v145_n1_p166_Linero
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Akt Argentine hemorrhagic fever Junín virus Ly294002 Phosphatidylinositol 3-kinase cell receptor phosphatidylinositol 3 kinase protein kinase B transferrin receptor animal cell article cell surface controlled study enzyme activation enzyme inhibition enzyme phosphorylation human human cell in vitro study Junin virus nonhuman persistent virus infection priority journal protein expression protein synthesis ultraviolet radiation Vero cell virus activation virus cell interaction virus entry virus infection virus replication 1-Phosphatidylinositol 3-Kinase Animals Cercopithecus aethiops Chromones Cricetinae Enzyme Inhibitors Hemorrhagic Fever, American Host-Pathogen Interactions Humans Junin virus Morpholines Phosphorylation Proto-Oncogene Proteins c-akt Signal Transduction Vero Cells Virus Internalization Virus Replication Arenavirus Junin virus
spellingShingle	Akt Argentine hemorrhagic fever Junín virus Ly294002 Phosphatidylinositol 3-kinase cell receptor phosphatidylinositol 3 kinase protein kinase B transferrin receptor animal cell article cell surface controlled study enzyme activation enzyme inhibition enzyme phosphorylation human human cell in vitro study Junin virus nonhuman persistent virus infection priority journal protein expression protein synthesis ultraviolet radiation Vero cell virus activation virus cell interaction virus entry virus infection virus replication 1-Phosphatidylinositol 3-Kinase Animals Cercopithecus aethiops Chromones Cricetinae Enzyme Inhibitors Hemorrhagic Fever, American Host-Pathogen Interactions Humans Junin virus Morpholines Phosphorylation Proto-Oncogene Proteins c-akt Signal Transduction Vero Cells Virus Internalization Virus Replication Arenavirus Junin virus Linero, Florencia N. Scolaro, Luis Alberto Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro
topic_facet	Akt Argentine hemorrhagic fever Junín virus Ly294002 Phosphatidylinositol 3-kinase cell receptor phosphatidylinositol 3 kinase protein kinase B transferrin receptor animal cell article cell surface controlled study enzyme activation enzyme inhibition enzyme phosphorylation human human cell in vitro study Junin virus nonhuman persistent virus infection priority journal protein expression protein synthesis ultraviolet radiation Vero cell virus activation virus cell interaction virus entry virus infection virus replication 1-Phosphatidylinositol 3-Kinase Animals Cercopithecus aethiops Chromones Cricetinae Enzyme Inhibitors Hemorrhagic Fever, American Host-Pathogen Interactions Humans Junin virus Morpholines Phosphorylation Proto-Oncogene Proteins c-akt Signal Transduction Vero Cells Virus Internalization Virus Replication Arenavirus Junin virus
description	In this paper we demonstrate that infection of cell cultures with the arenavirus Junín (JUNV), agent of the argentine haemorrhagic fever, leads to the activation of PI3K/Akt signalling pathway. Phosphorylation of Akt occurs early during JUNV infection of Vero cells and is blocked by the PI3K inhibitor, Ly294002. Infection of cells with UV-irradiated JUNV redeemed the pattern of stimulation observed for infectious virus indicating that an early stage of multiplication cycle would be enough to trigger activation. Treatment of cells with chlorpromazine abrogated phosphorylation of Akt upon JUNV infection suggesting virus internalization as responsible for activation. Inhibition of Akt phosphorylation by Ly294002 impaired viral protein synthesis and expression leading to a reduced infectious virus yield without blocking the onset of persistent stage of infection. This impairment is linked to a reduced amount of virus bound to cells probably due to a blockage on the recycling of transferrin cell-receptor, employed by the virus to adsorb to the cell surface. Early Akt activation was also observed in BHK-21 and A549 JUNV infected cells suggesting an important role of PI3K/Akt signalling in JUNV multiplication in vitro. © 2009 Elsevier B.V. All rights reserved.
author	Linero, Florencia N. Scolaro, Luis Alberto
author_facet	Linero, Florencia N. Scolaro, Luis Alberto
author_sort	Linero, Florencia N.
title	Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro
title_short	Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro
title_full	Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro
title_fullStr	Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro
title_full_unstemmed	Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro
title_sort	participation of the phosphatidylinositol 3-kinase/akt pathway in junín virus replication in vitro
publishDate	2009
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v145_n1_p166_Linero http://hdl.handle.net/20.500.12110/paper_01681702_v145_n1_p166_Linero
work_keys_str_mv	AT lineroflorencian participationofthephosphatidylinositol3kinaseaktpathwayinjuninvirusreplicationinvitro AT scolaroluisalberto participationofthephosphatidylinositol3kinaseaktpathwayinjuninvirusreplicationinvitro
_version_	1768543556417355776

Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junín virus replication in vitro

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