Characterization of Junín virus particles inactivated by a zinc finger-reactive compound
Our previous studies reported the inhibitory action against arenaviruses of antiretroviral zinc finger-reactive compounds provided by the National Cancer Institute (USA). These compounds were able to inactivate virions as well as to reduce virus yields from infected cells. Here, the inactivation of...
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paper:paper_01681702_v143_n1_p106_Garcia2023-06-08T15:17:37Z Characterization of Junín virus particles inactivated by a zinc finger-reactive compound García, Cybele Carina Ellenberg, Paula C. Artuso, María Carolina Scolaro, Luis Alberto Damonte, Elsa Beatriz Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Junin virus Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers Arenavirus Junin virus Our previous studies reported the inhibitory action against arenaviruses of antiretroviral zinc finger-reactive compounds provided by the National Cancer Institute (USA). These compounds were able to inactivate virions as well as to reduce virus yields from infected cells. Here, the inactivation of the arenavirus Junín (JUNV), agent of Argentine hemorrhagic fever, by the aromatic disulfide NSC20625 was analyzed. The treatment of purified JUNV with this compound eliminated infectivity apparently through irreversible modifications in the matrix Z protein detected by: (a) alterations in the electrophoretic migration profile of Z under non-reducing conditions; (b) an electrodense labeling in the internal layer beneath the envelope and around the matrix Z protein, in negatively stained preparations; (c) changes in the subcellular localization of Z in cells transfected with a recombinant fusion protein JUNVZ-eGFP. The infection of Vero cells with JUNV inactivated particles was blocked at the uncoating of viral nucleocapsid from endosomes, providing new evidence for a functional role of Z in this stage of arenavirus cycle. Furthermore, the inactivated JUNV particles retained the immunoreactivity of the surface glycoprotein GP1 suggesting that this disulfide may be useful in the pursuit of an inactivating agent to obtain a vaccine antigen or diagnostic tool. © 2009 Elsevier B.V. All rights reserved. Fil:García, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ellenberg, P.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Artuso, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v143_n1_p106_Garcia http://hdl.handle.net/20.500.12110/paper_01681702_v143_n1_p106_Garcia |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Junin virus Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers Arenavirus Junin virus |
spellingShingle |
Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Junin virus Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers Arenavirus Junin virus García, Cybele Carina Ellenberg, Paula C. Artuso, María Carolina Scolaro, Luis Alberto Damonte, Elsa Beatriz Characterization of Junín virus particles inactivated by a zinc finger-reactive compound |
topic_facet |
Arenavirus Junín virus Matrix protein Virus inactivation Zinc finger disulfide disulfide nsc20625 enhanced green fluorescent protein fatty acid binding protein hybrid protein matrix protein unclassified drug animal cell article cellular distribution controlled study electrophoresis endosome genetic transfection hemorrhagic fever Junin virus nonhuman priority journal virogenesis virus inactivation virus infectivity virus nucleocapsid virus particle zinc finger motif Animals Anti-HIV Agents Arenaviridae Infections Azo Compounds Carrier Proteins Cercopithecus aethiops Disulfides Green Fluorescent Proteins Junin virus Microscopy, Electron, Transmission Nucleocapsid Proteins Recombinant Fusion Proteins Vero Cells Virion Virus Inactivation Zinc Fingers Arenavirus Junin virus |
description |
Our previous studies reported the inhibitory action against arenaviruses of antiretroviral zinc finger-reactive compounds provided by the National Cancer Institute (USA). These compounds were able to inactivate virions as well as to reduce virus yields from infected cells. Here, the inactivation of the arenavirus Junín (JUNV), agent of Argentine hemorrhagic fever, by the aromatic disulfide NSC20625 was analyzed. The treatment of purified JUNV with this compound eliminated infectivity apparently through irreversible modifications in the matrix Z protein detected by: (a) alterations in the electrophoretic migration profile of Z under non-reducing conditions; (b) an electrodense labeling in the internal layer beneath the envelope and around the matrix Z protein, in negatively stained preparations; (c) changes in the subcellular localization of Z in cells transfected with a recombinant fusion protein JUNVZ-eGFP. The infection of Vero cells with JUNV inactivated particles was blocked at the uncoating of viral nucleocapsid from endosomes, providing new evidence for a functional role of Z in this stage of arenavirus cycle. Furthermore, the inactivated JUNV particles retained the immunoreactivity of the surface glycoprotein GP1 suggesting that this disulfide may be useful in the pursuit of an inactivating agent to obtain a vaccine antigen or diagnostic tool. © 2009 Elsevier B.V. All rights reserved. |
author |
García, Cybele Carina Ellenberg, Paula C. Artuso, María Carolina Scolaro, Luis Alberto Damonte, Elsa Beatriz |
author_facet |
García, Cybele Carina Ellenberg, Paula C. Artuso, María Carolina Scolaro, Luis Alberto Damonte, Elsa Beatriz |
author_sort |
García, Cybele Carina |
title |
Characterization of Junín virus particles inactivated by a zinc finger-reactive compound |
title_short |
Characterization of Junín virus particles inactivated by a zinc finger-reactive compound |
title_full |
Characterization of Junín virus particles inactivated by a zinc finger-reactive compound |
title_fullStr |
Characterization of Junín virus particles inactivated by a zinc finger-reactive compound |
title_full_unstemmed |
Characterization of Junín virus particles inactivated by a zinc finger-reactive compound |
title_sort |
characterization of junín virus particles inactivated by a zinc finger-reactive compound |
publishDate |
2009 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v143_n1_p106_Garcia http://hdl.handle.net/20.500.12110/paper_01681702_v143_n1_p106_Garcia |
work_keys_str_mv |
AT garciacybelecarina characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound AT ellenbergpaulac characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound AT artusomariacarolina characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound AT scolaroluisalberto characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound AT damonteelsabeatriz characterizationofjuninvirusparticlesinactivatedbyazincfingerreactivecompound |
_version_ |
1768543320486707200 |