Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells

C167, a mutant derived from the XJC13 strain of Junin virus, is highly attenuated in its pathogenic properties for newborn mice. Whereas 102 PFU of XJC13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. Survival of mice infected with C167 was assoc...

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Autores principales: Scolaro, Luis Alberto, Mersich, Susana E., Damonte, Elsa Beatriz
Publicado: 1989
Materias:
Arenavirus
Argentine hemorrhagic fever
Attenuated mutant
Junin virus
Mouse virulence
Restricted multiplication in murine cells
animal experiment
arenavirus
cell culture
junin virus
mouse
nonhuman
priority journal
virus attenuation
virus mutant
virus replication
virus virulence
Animal
Animals, Newborn
Arenaviridae
Arenaviruses, New World
Hemorrhagic Fever, American
Interferons
Mice
Mutation
Support, Non-U.S. Gov't
Virulence
Virus Replication
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v13_n4_p283_Scolaro
http://hdl.handle.net/20.500.12110/paper_01681702_v13_n4_p283_Scolaro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01681702_v13_n4_p283_Scolaro
record_format dspace
spelling paper:paper_01681702_v13_n4_p283_Scolaro2023-06-08T15:17:36Z Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells Scolaro, Luis Alberto Mersich, Susana E. Damonte, Elsa Beatriz Arenavirus Argentine hemorrhagic fever Attenuated mutant Junin virus Mouse virulence Restricted multiplication in murine cells animal experiment arenavirus cell culture junin virus mouse nonhuman priority journal virus attenuation virus mutant virus replication virus virulence Animal Animals, Newborn Arenaviridae Arenaviruses, New World Hemorrhagic Fever, American Interferons Mice Mutation Support, Non-U.S. Gov't Virulence Virus Replication C167, a mutant derived from the XJC13 strain of Junin virus, is highly attenuated in its pathogenic properties for newborn mice. Whereas 102 PFU of XJC13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. Survival of mice infected with C167 was associated with a reduced and delayed virus replication in brain and a defective spread of virus from the site of inoculation to the other tissues, including spleen, kidney, thymus, liver, peritoneal cells and serum. As an apparent consequence of the restricted replication of C167 in mice, no detectable interferon induction and low levels of neutralizing antibodies were observed. Analysis of multiplication kinetics of C167 and XJC13 in different cell cultures in vitro has confirmed that the attenuated phenotype of C167 was related to a specific inefficient replication in murine cells. This host-range restriction was due to a combination of adsorption and penetration blockage. © 1988. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mersich, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v13_n4_p283_Scolaro http://hdl.handle.net/20.500.12110/paper_01681702_v13_n4_p283_Scolaro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Arenavirus
Argentine hemorrhagic fever
Attenuated mutant
Junin virus
Mouse virulence
Restricted multiplication in murine cells
animal experiment
arenavirus
cell culture
junin virus
mouse
nonhuman
priority journal
virus attenuation
virus mutant
virus replication
virus virulence
Animal
Animals, Newborn
Arenaviridae
Arenaviruses, New World
Hemorrhagic Fever, American
Interferons
Mice
Mutation
Support, Non-U.S. Gov't
Virulence
Virus Replication
spellingShingle Arenavirus
Argentine hemorrhagic fever
Attenuated mutant
Junin virus
Mouse virulence
Restricted multiplication in murine cells
animal experiment
arenavirus
cell culture
junin virus
mouse
nonhuman
priority journal
virus attenuation
virus mutant
virus replication
virus virulence
Animal
Animals, Newborn
Arenaviridae
Arenaviruses, New World
Hemorrhagic Fever, American
Interferons
Mice
Mutation
Support, Non-U.S. Gov't
Virulence
Virus Replication
Scolaro, Luis Alberto
Mersich, Susana E.
Damonte, Elsa Beatriz
Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells
topic_facet Arenavirus
Argentine hemorrhagic fever
Attenuated mutant
Junin virus
Mouse virulence
Restricted multiplication in murine cells
animal experiment
arenavirus
cell culture
junin virus
mouse
nonhuman
priority journal
virus attenuation
virus mutant
virus replication
virus virulence
Animal
Animals, Newborn
Arenaviridae
Arenaviruses, New World
Hemorrhagic Fever, American
Interferons
Mice
Mutation
Support, Non-U.S. Gov't
Virulence
Virus Replication
description C167, a mutant derived from the XJC13 strain of Junin virus, is highly attenuated in its pathogenic properties for newborn mice. Whereas 102 PFU of XJC13 injected intracerebrally killed 100% of two-day-old mice, the mutant showed no detectable lethality. Survival of mice infected with C167 was associated with a reduced and delayed virus replication in brain and a defective spread of virus from the site of inoculation to the other tissues, including spleen, kidney, thymus, liver, peritoneal cells and serum. As an apparent consequence of the restricted replication of C167 in mice, no detectable interferon induction and low levels of neutralizing antibodies were observed. Analysis of multiplication kinetics of C167 and XJC13 in different cell cultures in vitro has confirmed that the attenuated phenotype of C167 was related to a specific inefficient replication in murine cells. This host-range restriction was due to a combination of adsorption and penetration blockage. © 1988.
author Scolaro, Luis Alberto
Mersich, Susana E.
Damonte, Elsa Beatriz
author_facet Scolaro, Luis Alberto
Mersich, Susana E.
Damonte, Elsa Beatriz
author_sort Scolaro, Luis Alberto
title Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells
title_short Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells
title_full Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells
title_fullStr Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells
title_full_unstemmed Reduced virulence of a Junin virus mutant is associated with restricted multiplication in murine cells
title_sort reduced virulence of a junin virus mutant is associated with restricted multiplication in murine cells
publishDate 1989
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v13_n4_p283_Scolaro
http://hdl.handle.net/20.500.12110/paper_01681702_v13_n4_p283_Scolaro
work_keys_str_mv AT scolaroluisalberto reducedvirulenceofajuninvirusmutantisassociatedwithrestrictedmultiplicationinmurinecells
AT mersichsusanae reducedvirulenceofajuninvirusmutantisassociatedwithrestrictedmultiplicationinmurinecells
AT damonteelsabeatriz reducedvirulenceofajuninvirusmutantisassociatedwithrestrictedmultiplicationinmurinecells
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