Involvement of cytoskeleton in Junín virus entry
The early events in Junín virus (JUNV) infection are not thoroughly understood. We have previously shown that JUNV enter cells by clathrin-mediated endocytosis. In this report we examine the role of microfilaments and microtubules during early virus infection. Inhibitory effects of drugs affecting m...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v138_n1-2_p17_Martinez http://hdl.handle.net/20.500.12110/paper_01681702_v138_n1-2_p17_Martinez |
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paper:paper_01681702_v138_n1-2_p17_Martinez2023-06-08T15:17:36Z Involvement of cytoskeleton in Junín virus entry Martínez, María Guadalupe Cordo, Sandra M. Candurra, Nélida Alicia Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Cytoskeleton Depsipeptides Humans Junin virus Thiazolidines Vero Cells Virus Internalization Arenavirus Junin virus The early events in Junín virus (JUNV) infection are not thoroughly understood. We have previously shown that JUNV enter cells by clathrin-mediated endocytosis. In this report we examine the role of microfilaments and microtubules during early virus infection. Inhibitory effects of drugs affecting main cytoskeletal components on JUNV entry into Vero cells were analyzed. Drugs that disrupted microfilaments or stabilized microtubules inhibited early steps of virus entry. In contrast, drugs that stabilized microfilaments or depolymerized microtubules were not able to block virus entry very efficiently. Furthermore, real time PCR was performed to detect viral entry and we found more than 10-fold less RNA when microfilaments were depolymerized while a 100-fold diminution was seen when microtubules were stabilized. Taken together our results demonstrate that JUNV relies on an intact actin network during early infection in Vero cells while a dynamic microtubule network is also needed. This represents an important contribution to the characterization of arenavirus multiplication cycle. © 2008 Elsevier B.V. All rights reserved. Fil:Martinez, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cordo, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v138_n1-2_p17_Martinez http://hdl.handle.net/20.500.12110/paper_01681702_v138_n1-2_p17_Martinez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Cytoskeleton Depsipeptides Humans Junin virus Thiazolidines Vero Cells Virus Internalization Arenavirus Junin virus |
spellingShingle |
Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Cytoskeleton Depsipeptides Humans Junin virus Thiazolidines Vero Cells Virus Internalization Arenavirus Junin virus Martínez, María Guadalupe Cordo, Sandra M. Candurra, Nélida Alicia Involvement of cytoskeleton in Junín virus entry |
topic_facet |
Actin Cytoskeleton Junín virus Tubulin cytochalasin D jaspamide latrunculin A nocodazole paclitaxel virus RNA animal cell article controlled study Junin virus microfilament microtubule nonhuman priority journal reverse transcription polymerase chain reaction RNA synthesis Vero cell virus entry virus infection Animals Arenaviridae Infections Bicyclo Compounds, Heterocyclic Cercopithecus aethiops Cytoskeleton Depsipeptides Humans Junin virus Thiazolidines Vero Cells Virus Internalization Arenavirus Junin virus |
description |
The early events in Junín virus (JUNV) infection are not thoroughly understood. We have previously shown that JUNV enter cells by clathrin-mediated endocytosis. In this report we examine the role of microfilaments and microtubules during early virus infection. Inhibitory effects of drugs affecting main cytoskeletal components on JUNV entry into Vero cells were analyzed. Drugs that disrupted microfilaments or stabilized microtubules inhibited early steps of virus entry. In contrast, drugs that stabilized microfilaments or depolymerized microtubules were not able to block virus entry very efficiently. Furthermore, real time PCR was performed to detect viral entry and we found more than 10-fold less RNA when microfilaments were depolymerized while a 100-fold diminution was seen when microtubules were stabilized. Taken together our results demonstrate that JUNV relies on an intact actin network during early infection in Vero cells while a dynamic microtubule network is also needed. This represents an important contribution to the characterization of arenavirus multiplication cycle. © 2008 Elsevier B.V. All rights reserved. |
author |
Martínez, María Guadalupe Cordo, Sandra M. Candurra, Nélida Alicia |
author_facet |
Martínez, María Guadalupe Cordo, Sandra M. Candurra, Nélida Alicia |
author_sort |
Martínez, María Guadalupe |
title |
Involvement of cytoskeleton in Junín virus entry |
title_short |
Involvement of cytoskeleton in Junín virus entry |
title_full |
Involvement of cytoskeleton in Junín virus entry |
title_fullStr |
Involvement of cytoskeleton in Junín virus entry |
title_full_unstemmed |
Involvement of cytoskeleton in Junín virus entry |
title_sort |
involvement of cytoskeleton in junín virus entry |
publishDate |
2008 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681702_v138_n1-2_p17_Martinez http://hdl.handle.net/20.500.12110/paper_01681702_v138_n1-2_p17_Martinez |
work_keys_str_mv |
AT martinezmariaguadalupe involvementofcytoskeletoninjuninvirusentry AT cordosandram involvementofcytoskeletoninjuninvirusentry AT candurranelidaalicia involvementofcytoskeletoninjuninvirusentry |
_version_ |
1768541558340059136 |