Sol-gel silica platforms for microalgae-based optical biosensors
An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamyd...
Guardado en:
| Autor principal: | |
|---|---|
| Publicado: |
2014
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v179_n1_p65_Perullini http://hdl.handle.net/20.500.12110/paper_01681656_v179_n1_p65_Perullini |
| Aporte de: |
| id |
paper:paper_01681656_v179_n1_p65_Perullini |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_01681656_v179_n1_p65_Perullini2023-06-08T15:17:33Z Sol-gel silica platforms for microalgae-based optical biosensors Perullini, Ana Mercedes Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlamydomonas reinhardtii Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering water pollutant X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Microalgae Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the two-step procedure. Tuning the alginate protecting function with the aid of Tris-HCl buffer, the sol-gel synthesis was conducted at pH 4.0 well below the tolerance limit imposed by the encapsulated microalgae. The acidic condensation of Si(IV) generates silica matrices with outstanding optical properties that suit the requirements of biosensors based on optical detection methods. © 2014 Elsevier B.V. Fil:Perullini, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v179_n1_p65_Perullini http://hdl.handle.net/20.500.12110/paper_01681656_v179_n1_p65_Perullini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlamydomonas reinhardtii Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering water pollutant X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Microalgae Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction |
| spellingShingle |
Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlamydomonas reinhardtii Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering water pollutant X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Microalgae Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction Perullini, Ana Mercedes Sol-gel silica platforms for microalgae-based optical biosensors |
| topic_facet |
Biosensor Esterase activity Microalgae Optical quality Sol-gel Algae Microorganisms Silica Sol-gel process Sol-gels Biosensing platforms Chlamydomonas reinhardtii Esterase activities Micro-algae Optical bio-sensors Optical detection methods Optical qualities Pseudokirchneriella subcapitata Biosensors alginic acid calcium alginate silicate silicon dioxide tetraethoxysilane esterase glucuronic acid hexuronic acid silica gel water pollutant algal growth article biocompatibility Chlamydomonas reinhardtii Chlorella vulgaris elementary particle encapsulation fluorescence gelation growth inhibition hydrogel nonhuman optical biosensor pH polymerization priority journal Selenastrum capricornutum synthesis chemistry enzymology genetic procedures isolation and purification metabolism microalga procedures small angle scattering water pollutant X ray diffraction Alginates Biosensing Techniques Esterases Glucuronic Acid Hexuronic Acids Microalgae Scattering, Small Angle Silica Gel Water Pollutants, Chemical X-Ray Diffraction |
| description |
An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the two-step procedure. Tuning the alginate protecting function with the aid of Tris-HCl buffer, the sol-gel synthesis was conducted at pH 4.0 well below the tolerance limit imposed by the encapsulated microalgae. The acidic condensation of Si(IV) generates silica matrices with outstanding optical properties that suit the requirements of biosensors based on optical detection methods. © 2014 Elsevier B.V. |
| author |
Perullini, Ana Mercedes |
| author_facet |
Perullini, Ana Mercedes |
| author_sort |
Perullini, Ana Mercedes |
| title |
Sol-gel silica platforms for microalgae-based optical biosensors |
| title_short |
Sol-gel silica platforms for microalgae-based optical biosensors |
| title_full |
Sol-gel silica platforms for microalgae-based optical biosensors |
| title_fullStr |
Sol-gel silica platforms for microalgae-based optical biosensors |
| title_full_unstemmed |
Sol-gel silica platforms for microalgae-based optical biosensors |
| title_sort |
sol-gel silica platforms for microalgae-based optical biosensors |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v179_n1_p65_Perullini http://hdl.handle.net/20.500.12110/paper_01681656_v179_n1_p65_Perullini |
| work_keys_str_mv |
AT perullinianamercedes solgelsilicaplatformsformicroalgaebasedopticalbiosensors |
| _version_ |
1768545461375860736 |