Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens

Solanum tuberosum plants were transformed with three genetic constructions expressing the Nicotiana tabacum AP24 osmotine, Phyllomedusa sauvagii dermaseptin and Gallus gallus lysozyme, and with a double-transgene construction expressing the AP24 and lysozyme sequences. Re-transformation of dermasept...

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Autores principales:	Rivero Pérez, María Mercedes, Furman, Nicolás Federico, Picca, Pablo Ignacio, Lentz, Ezequiel Matías, Mentaberry, Alejandro Néstor
Publicado:	2012
Materias:	AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Resistance Rhizoctonia solani Streptomyces scabies Transgenic Transgenics Bacteria Electric resistance Enzymes Fungi Genes dermaseptin lysozyme amino acid sequence antibacterial activity antibiotic resistance antifungal resistance article bacterial gene bacterial infection chicken controlled study fowl frog fungus growth gene expression genetic transformation growth inhibition Hyphomycetes in vitro study inoculation microbial genetics nonhuman nucleotide sequence Pectobacterium carotovorum phenotype plant leaf potato priority journal quantitative analysis Streptomyces tobacco transgene transgenic plant Amphibian Proteins Animals Antimicrobial Cationic Peptides Chickens Gene Expression Regulation, Plant Muramidase Plant Diseases Plant Proteins Plants, Genetically Modified Solanum tuberosum Tobacco Bacteria (microorganisms) Gallus gallus Nicotiana tabacum Phyllomedusa sauvagei Streptomyces scabiei Thanatephorus cucumeris
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v157_n2_p334_Rivero http://hdl.handle.net/20.500.12110/paper_01681656_v157_n2_p334_Rivero
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_01681656_v157_n2_p334_Rivero2023-06-08T15:17:32Z Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens Rivero Pérez, María Mercedes Furman, Nicolás Federico Picca, Pablo Ignacio Lentz, Ezequiel Matías Mentaberry, Alejandro Néstor AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Resistance Rhizoctonia solani Streptomyces scabies Transgenic AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Rhizoctonia solani Streptomyces scabies Transgenics Bacteria Electric resistance Enzymes Fungi Genes dermaseptin lysozyme amino acid sequence antibacterial activity antibiotic resistance antifungal resistance article bacterial gene bacterial infection chicken controlled study fowl frog fungus growth Fusarium solani gene expression genetic transformation growth inhibition Hyphomycetes in vitro study inoculation microbial genetics nonhuman nucleotide sequence Pectobacterium carotovorum phenotype Phytophthora infestans plant leaf potato priority journal quantitative analysis Streptomyces tobacco transgene transgenic plant Amphibian Proteins Animals Antimicrobial Cationic Peptides Bacteria Chickens Fungi Gene Expression Regulation, Plant Muramidase Pectobacterium carotovorum Plant Diseases Plant Proteins Plants, Genetically Modified Solanum tuberosum Tobacco Bacteria (microorganisms) Fusarium solani Gallus gallus Nicotiana tabacum Pectobacterium carotovorum Phyllomedusa sauvagei Phytophthora infestans Solanum tuberosum Streptomyces scabiei Thanatephorus cucumeris Solanum tuberosum plants were transformed with three genetic constructions expressing the Nicotiana tabacum AP24 osmotine, Phyllomedusa sauvagii dermaseptin and Gallus gallus lysozyme, and with a double-transgene construction expressing the AP24 and lysozyme sequences. Re-transformation of dermaseptin-transformed plants with the AP24/lysozyme construction allowed selection of plants simultaneously expressing the three transgenes. Potato lines expressing individual transgenes or double- and triple-transgene combinations were assayed for resistance to Erwinia carotovora using whole-plant and tuber infection assays. Resistance levels for both infection tests compared consistently for most potato lines and allowed selection of highly resistant phenotypes. Higher resistance levels were found in lines carrying the dermaseptin and lysozyme sequences, indicating that theses proteins are the major contributors to antibacterial activity. Similar results were obtained in tuber infection tests conducted with Streptomyces scabies. Plant lines showing the higher resistance to bacterial infections were challenged with Phytophthora infestans, Rhizoctonia solani and Fusarium solani. Considerable levels of resistance to each of these pathogens were evidenced employing semi-quantitative tests based in detached-leaf inoculation, fungal growth inhibition and in vitro plant inoculation. On the basis of these results, we propose that stacking of these transgenes is a promising approach to achieve resistance to both bacterial and fungal pathogens. © 2011 Elsevier B.V. Fil:Rivero, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Furman, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Picca, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lentz, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mentaberry, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v157_n2_p334_Rivero http://hdl.handle.net/20.500.12110/paper_01681656_v157_n2_p334_Rivero
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Resistance Rhizoctonia solani Streptomyces scabies Transgenic AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Rhizoctonia solani Streptomyces scabies Transgenics Bacteria Electric resistance Enzymes Fungi Genes dermaseptin lysozyme amino acid sequence antibacterial activity antibiotic resistance antifungal resistance article bacterial gene bacterial infection chicken controlled study fowl frog fungus growth Fusarium solani gene expression genetic transformation growth inhibition Hyphomycetes in vitro study inoculation microbial genetics nonhuman nucleotide sequence Pectobacterium carotovorum phenotype Phytophthora infestans plant leaf potato priority journal quantitative analysis Streptomyces tobacco transgene transgenic plant Amphibian Proteins Animals Antimicrobial Cationic Peptides Bacteria Chickens Fungi Gene Expression Regulation, Plant Muramidase Pectobacterium carotovorum Plant Diseases Plant Proteins Plants, Genetically Modified Solanum tuberosum Tobacco Bacteria (microorganisms) Fusarium solani Gallus gallus Nicotiana tabacum Pectobacterium carotovorum Phyllomedusa sauvagei Phytophthora infestans Solanum tuberosum Streptomyces scabiei Thanatephorus cucumeris
spellingShingle	AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Resistance Rhizoctonia solani Streptomyces scabies Transgenic AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Rhizoctonia solani Streptomyces scabies Transgenics Bacteria Electric resistance Enzymes Fungi Genes dermaseptin lysozyme amino acid sequence antibacterial activity antibiotic resistance antifungal resistance article bacterial gene bacterial infection chicken controlled study fowl frog fungus growth Fusarium solani gene expression genetic transformation growth inhibition Hyphomycetes in vitro study inoculation microbial genetics nonhuman nucleotide sequence Pectobacterium carotovorum phenotype Phytophthora infestans plant leaf potato priority journal quantitative analysis Streptomyces tobacco transgene transgenic plant Amphibian Proteins Animals Antimicrobial Cationic Peptides Bacteria Chickens Fungi Gene Expression Regulation, Plant Muramidase Pectobacterium carotovorum Plant Diseases Plant Proteins Plants, Genetically Modified Solanum tuberosum Tobacco Bacteria (microorganisms) Fusarium solani Gallus gallus Nicotiana tabacum Pectobacterium carotovorum Phyllomedusa sauvagei Phytophthora infestans Solanum tuberosum Streptomyces scabiei Thanatephorus cucumeris Rivero Pérez, María Mercedes Furman, Nicolás Federico Picca, Pablo Ignacio Lentz, Ezequiel Matías Mentaberry, Alejandro Néstor Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens
topic_facet	AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Resistance Rhizoctonia solani Streptomyces scabies Transgenic AP24 osmotine Dermaseptin Erwinia carotovora Fusarium solani Lysozyme Phytophthora infestans Potato Rhizoctonia solani Streptomyces scabies Transgenics Bacteria Electric resistance Enzymes Fungi Genes dermaseptin lysozyme amino acid sequence antibacterial activity antibiotic resistance antifungal resistance article bacterial gene bacterial infection chicken controlled study fowl frog fungus growth Fusarium solani gene expression genetic transformation growth inhibition Hyphomycetes in vitro study inoculation microbial genetics nonhuman nucleotide sequence Pectobacterium carotovorum phenotype Phytophthora infestans plant leaf potato priority journal quantitative analysis Streptomyces tobacco transgene transgenic plant Amphibian Proteins Animals Antimicrobial Cationic Peptides Bacteria Chickens Fungi Gene Expression Regulation, Plant Muramidase Pectobacterium carotovorum Plant Diseases Plant Proteins Plants, Genetically Modified Solanum tuberosum Tobacco Bacteria (microorganisms) Fusarium solani Gallus gallus Nicotiana tabacum Pectobacterium carotovorum Phyllomedusa sauvagei Phytophthora infestans Solanum tuberosum Streptomyces scabiei Thanatephorus cucumeris
description	Solanum tuberosum plants were transformed with three genetic constructions expressing the Nicotiana tabacum AP24 osmotine, Phyllomedusa sauvagii dermaseptin and Gallus gallus lysozyme, and with a double-transgene construction expressing the AP24 and lysozyme sequences. Re-transformation of dermaseptin-transformed plants with the AP24/lysozyme construction allowed selection of plants simultaneously expressing the three transgenes. Potato lines expressing individual transgenes or double- and triple-transgene combinations were assayed for resistance to Erwinia carotovora using whole-plant and tuber infection assays. Resistance levels for both infection tests compared consistently for most potato lines and allowed selection of highly resistant phenotypes. Higher resistance levels were found in lines carrying the dermaseptin and lysozyme sequences, indicating that theses proteins are the major contributors to antibacterial activity. Similar results were obtained in tuber infection tests conducted with Streptomyces scabies. Plant lines showing the higher resistance to bacterial infections were challenged with Phytophthora infestans, Rhizoctonia solani and Fusarium solani. Considerable levels of resistance to each of these pathogens were evidenced employing semi-quantitative tests based in detached-leaf inoculation, fungal growth inhibition and in vitro plant inoculation. On the basis of these results, we propose that stacking of these transgenes is a promising approach to achieve resistance to both bacterial and fungal pathogens. © 2011 Elsevier B.V.
author	Rivero Pérez, María Mercedes Furman, Nicolás Federico Picca, Pablo Ignacio Lentz, Ezequiel Matías Mentaberry, Alejandro Néstor
author_facet	Rivero Pérez, María Mercedes Furman, Nicolás Federico Picca, Pablo Ignacio Lentz, Ezequiel Matías Mentaberry, Alejandro Néstor
author_sort	Rivero Pérez, María Mercedes
title	Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens
title_short	Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens
title_full	Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens
title_fullStr	Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens
title_full_unstemmed	Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens
title_sort	stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens
publishDate	2012
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681656_v157_n2_p334_Rivero http://hdl.handle.net/20.500.12110/paper_01681656_v157_n2_p334_Rivero
work_keys_str_mv	AT riveroperezmariamercedes stackingofantimicrobialgenesinpotatotransgenicplantsconfersincreasedresistancetobacterialandfungalpathogens AT furmannicolasfederico stackingofantimicrobialgenesinpotatotransgenicplantsconfersincreasedresistancetobacterialandfungalpathogens AT piccapabloignacio stackingofantimicrobialgenesinpotatotransgenicplantsconfersincreasedresistancetobacterialandfungalpathogens AT lentzezequielmatias stackingofantimicrobialgenesinpotatotransgenicplantsconfersincreasedresistancetobacterialandfungalpathogens AT mentaberryalejandronestor stackingofantimicrobialgenesinpotatotransgenicplantsconfersincreasedresistancetobacterialandfungalpathogens
_version_	1768544128905248768

Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens

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