AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree
Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolat...
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paper:paper_01681605_v145_n2-3_p414_Somma2023-06-08T15:17:18Z AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree Pardo, Alejandro Guillermo A. arborescens Alternaria alternata/tenuissima Alternariol Monomethyl ether Tenuazonic acid Tomato black mould alternariol mycotoxin tenuazonic acid Alternaria Alternaria arborescens amplified fragment length polymorphism Argentina article fungal strain fungus culture fungus isolation genetic variability nonhuman pathogenicity rice tomato Alternaria Amplified Fragment Length Polymorphism Analysis Argentina DNA, Fungal Fruit Genetic Variation Lactones Lycopersicon esculentum Mycotoxins Plant Diseases Sequence Analysis, DNA Tenuazonic Acid Alternaria Alternaria arborescens Lycopersicon esculentum Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to each AFLP cluster, when cultured on rice, produced tenuazonic acid (up to 46,760. mg/kg), alternariol monomethyl ether (AME, up to 1860. mg/kg), and alternariol (up to 70. mg/kg). The toxin profile related to the strains was not related to any AFLP cluster, except for AME which was produced at lower level by A. arborescens. Most of strains were pathogenic on two types of commonly cultivated tomato fruits. These findings provide new information on the variability within the Alternaria species complex associated with tomato disease. © 2011 Elsevier B.V. Fil:Pardo, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v145_n2-3_p414_Somma http://hdl.handle.net/20.500.12110/paper_01681605_v145_n2-3_p414_Somma |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
A. arborescens Alternaria alternata/tenuissima Alternariol Monomethyl ether Tenuazonic acid Tomato black mould alternariol mycotoxin tenuazonic acid Alternaria Alternaria arborescens amplified fragment length polymorphism Argentina article fungal strain fungus culture fungus isolation genetic variability nonhuman pathogenicity rice tomato Alternaria Amplified Fragment Length Polymorphism Analysis Argentina DNA, Fungal Fruit Genetic Variation Lactones Lycopersicon esculentum Mycotoxins Plant Diseases Sequence Analysis, DNA Tenuazonic Acid Alternaria Alternaria arborescens Lycopersicon esculentum |
| spellingShingle |
A. arborescens Alternaria alternata/tenuissima Alternariol Monomethyl ether Tenuazonic acid Tomato black mould alternariol mycotoxin tenuazonic acid Alternaria Alternaria arborescens amplified fragment length polymorphism Argentina article fungal strain fungus culture fungus isolation genetic variability nonhuman pathogenicity rice tomato Alternaria Amplified Fragment Length Polymorphism Analysis Argentina DNA, Fungal Fruit Genetic Variation Lactones Lycopersicon esculentum Mycotoxins Plant Diseases Sequence Analysis, DNA Tenuazonic Acid Alternaria Alternaria arborescens Lycopersicon esculentum Pardo, Alejandro Guillermo AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree |
| topic_facet |
A. arborescens Alternaria alternata/tenuissima Alternariol Monomethyl ether Tenuazonic acid Tomato black mould alternariol mycotoxin tenuazonic acid Alternaria Alternaria arborescens amplified fragment length polymorphism Argentina article fungal strain fungus culture fungus isolation genetic variability nonhuman pathogenicity rice tomato Alternaria Amplified Fragment Length Polymorphism Analysis Argentina DNA, Fungal Fruit Genetic Variation Lactones Lycopersicon esculentum Mycotoxins Plant Diseases Sequence Analysis, DNA Tenuazonic Acid Alternaria Alternaria arborescens Lycopersicon esculentum |
| description |
Large amounts of tomato fruits and derived products are produced in Argentina and may be contaminated by Alternaria toxins. Limited information is available on the genetic variability, toxigenicity, and pathogenicity of Alternaria strains occurring on tomato. We analyzed 65 Alternaria strains isolated in Argentina from tomato fruits affected by black mould and from tomato puree, using amplified fragment length polymorphisms (AFLPs) technique. AFLP analysis resolved the set of strains in 3 main clusters (DICE similarity values of 58 and 60%) corresponding to A. alternata/tenuissima (44 strains), A. arborescens (15 strains) and to an unknown group (6 strains). Most of the representative strains, belonging to each AFLP cluster, when cultured on rice, produced tenuazonic acid (up to 46,760. mg/kg), alternariol monomethyl ether (AME, up to 1860. mg/kg), and alternariol (up to 70. mg/kg). The toxin profile related to the strains was not related to any AFLP cluster, except for AME which was produced at lower level by A. arborescens. Most of strains were pathogenic on two types of commonly cultivated tomato fruits. These findings provide new information on the variability within the Alternaria species complex associated with tomato disease. © 2011 Elsevier B.V. |
| author |
Pardo, Alejandro Guillermo |
| author_facet |
Pardo, Alejandro Guillermo |
| author_sort |
Pardo, Alejandro Guillermo |
| title |
AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree |
| title_short |
AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree |
| title_full |
AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree |
| title_fullStr |
AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree |
| title_full_unstemmed |
AFLP variability, toxin production, and pathogenicity of Alternaria species from Argentinean tomato fruits and puree |
| title_sort |
aflp variability, toxin production, and pathogenicity of alternaria species from argentinean tomato fruits and puree |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01681605_v145_n2-3_p414_Somma http://hdl.handle.net/20.500.12110/paper_01681605_v145_n2-3_p414_Somma |
| work_keys_str_mv |
AT pardoalejandroguillermo aflpvariabilitytoxinproductionandpathogenicityofalternariaspeciesfromargentineantomatofruitsandpuree |
| _version_ |
1768544816291905536 |