New method for electroporation of Lactobacillus species grown in high salt

We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per micr...

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Autores principales:	Palomino, María Mercedes, Allievi, Mariana Claudia, Prado Acosta, Mariano, Ruzal, Sandra Mónica
Publicado:	2010
Materias:	Electroporation High salt growth Lactobacillus plasmid DNA sodium chloride article bacterial cell wall bacterial growth bacterial strain bacterium transformation controlled study cryopreservation electroporation fermentation Lactobacillus acidophilus Lactobacillus delbrueckii Lactobacillus paracasei Lactobacillus plantarum nonhuman priority journal salt tolerance Lactobacillus casei Lactobacillus delbrueckii subsp. lactis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01677012_v83_n2_p164_Palomino http://hdl.handle.net/20.500.12110/paper_01677012_v83_n2_p164_Palomino
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01677012_v83_n2_p164_Palomino
record_format	dspace
spelling	paper:paper_01677012_v83_n2_p164_Palomino2023-06-08T15:16:48Z New method for electroporation of Lactobacillus species grown in high salt Palomino, María Mercedes Allievi, Mariana Claudia Prado Acosta, Mariano Ruzal, Sandra Mónica Electroporation High salt growth Lactobacillus plasmid DNA sodium chloride article bacterial cell wall bacterial growth bacterial strain bacterium transformation controlled study cryopreservation electroporation fermentation Lactobacillus Lactobacillus acidophilus Lactobacillus delbrueckii Lactobacillus paracasei Lactobacillus plantarum nonhuman priority journal salt tolerance Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lactobacillus delbrueckii subsp. lactis Lactobacillus paracasei Lactobacillus plantarum We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per microgram of plasmid DNA was achieved following growth in the presence of 0.9M NaCl. Plasmids of different sizes and replication origins were also similarly transformed. These competent cells could be used either directly or stored frozen, up to 1month, for future use, with similar efficiency. This protocol was assayed with different Lactobacillus species: L. delbrueckii subsp. lactis, L. paracasei, L. plantarum and L. acidophilus, and it was found that they were transformed with similar efficiency. © 2010 Elsevier B.V. Fil:Palomino, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Allievi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Prado-Acosta, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ruzal, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01677012_v83_n2_p164_Palomino http://hdl.handle.net/20.500.12110/paper_01677012_v83_n2_p164_Palomino
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Electroporation High salt growth Lactobacillus plasmid DNA sodium chloride article bacterial cell wall bacterial growth bacterial strain bacterium transformation controlled study cryopreservation electroporation fermentation Lactobacillus Lactobacillus acidophilus Lactobacillus delbrueckii Lactobacillus paracasei Lactobacillus plantarum nonhuman priority journal salt tolerance Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lactobacillus delbrueckii subsp. lactis Lactobacillus paracasei Lactobacillus plantarum
spellingShingle	Electroporation High salt growth Lactobacillus plasmid DNA sodium chloride article bacterial cell wall bacterial growth bacterial strain bacterium transformation controlled study cryopreservation electroporation fermentation Lactobacillus Lactobacillus acidophilus Lactobacillus delbrueckii Lactobacillus paracasei Lactobacillus plantarum nonhuman priority journal salt tolerance Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lactobacillus delbrueckii subsp. lactis Lactobacillus paracasei Lactobacillus plantarum Palomino, María Mercedes Allievi, Mariana Claudia Prado Acosta, Mariano Ruzal, Sandra Mónica New method for electroporation of Lactobacillus species grown in high salt
topic_facet	Electroporation High salt growth Lactobacillus plasmid DNA sodium chloride article bacterial cell wall bacterial growth bacterial strain bacterium transformation controlled study cryopreservation electroporation fermentation Lactobacillus Lactobacillus acidophilus Lactobacillus delbrueckii Lactobacillus paracasei Lactobacillus plantarum nonhuman priority journal salt tolerance Lactobacillus Lactobacillus acidophilus Lactobacillus casei Lactobacillus delbrueckii subsp. lactis Lactobacillus paracasei Lactobacillus plantarum
description	We here describe a new method for electroporation of Lactobacillus species, obligately homofermentative and facultatively heterofermentative, based on the cell-wall weakening resulting from growth in high-salt media. For L. casei, optimum transformation efficiency of up to 105 transformants per microgram of plasmid DNA was achieved following growth in the presence of 0.9M NaCl. Plasmids of different sizes and replication origins were also similarly transformed. These competent cells could be used either directly or stored frozen, up to 1month, for future use, with similar efficiency. This protocol was assayed with different Lactobacillus species: L. delbrueckii subsp. lactis, L. paracasei, L. plantarum and L. acidophilus, and it was found that they were transformed with similar efficiency. © 2010 Elsevier B.V.
author	Palomino, María Mercedes Allievi, Mariana Claudia Prado Acosta, Mariano Ruzal, Sandra Mónica
author_facet	Palomino, María Mercedes Allievi, Mariana Claudia Prado Acosta, Mariano Ruzal, Sandra Mónica
author_sort	Palomino, María Mercedes
title	New method for electroporation of Lactobacillus species grown in high salt
title_short	New method for electroporation of Lactobacillus species grown in high salt
title_full	New method for electroporation of Lactobacillus species grown in high salt
title_fullStr	New method for electroporation of Lactobacillus species grown in high salt
title_full_unstemmed	New method for electroporation of Lactobacillus species grown in high salt
title_sort	new method for electroporation of lactobacillus species grown in high salt
publishDate	2010
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01677012_v83_n2_p164_Palomino http://hdl.handle.net/20.500.12110/paper_01677012_v83_n2_p164_Palomino
work_keys_str_mv	AT palominomariamercedes newmethodforelectroporationoflactobacillusspeciesgrowninhighsalt AT allievimarianaclaudia newmethodforelectroporationoflactobacillusspeciesgrowninhighsalt AT pradoacostamariano newmethodforelectroporationoflactobacillusspeciesgrowninhighsalt AT ruzalsandramonica newmethodforelectroporationoflactobacillusspeciesgrowninhighsalt
_version_	1768546536491319296

New method for electroporation of Lactobacillus species grown in high salt

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