Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability

We describe an encapsulation–dehydration procedure with prefreezing steps for the cryopreservation of rhizome bud explants of Asparagus officinalis L. cv. Morado de Huétor. With this procedure, survival of Rhizome buds was at least 84 and 42% developed to complete plantlets at 8 weeks. Flow cytometr...

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Detalles Bibliográficos
Publicado: 2018
Materias:
Asparagus
Cryopreservation
Encapsulation–dehydration
Flow cytometry
Molecular markers
Rhizome bud
Dehydration
Recovery
Sugar (sucrose)
Cryo-preservation
Dehydration procedure
Genetic stability
Long-term preservation
Molecular marker
Molecular stability
Biological materials preservation
Buds
Explants
Moisture Content
Peltogyne
Preservation
Stability
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v133_n3_p395_CarmonaMartin
http://hdl.handle.net/20.500.12110/paper_01676857_v133_n3_p395_CarmonaMartin
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01676857_v133_n3_p395_CarmonaMartin
record_format dspace
spelling paper:paper_01676857_v133_n3_p395_CarmonaMartin2023-06-08T15:16:45Z Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability Asparagus Cryopreservation Encapsulation–dehydration Flow cytometry Molecular markers Rhizome bud Dehydration Flow cytometry Recovery Sugar (sucrose) Asparagus Cryo-preservation Dehydration procedure Genetic stability Long-term preservation Molecular marker Molecular stability Rhizome bud Biological materials preservation Buds Dehydration Explants Moisture Content Peltogyne Preservation Recovery Stability We describe an encapsulation–dehydration procedure with prefreezing steps for the cryopreservation of rhizome bud explants of Asparagus officinalis L. cv. Morado de Huétor. With this procedure, survival of Rhizome buds was at least 84 and 42% developed to complete plantlets at 8 weeks. Flow cytometry and EST-SSR molecular markers were used to assess genetic stability of the regenerated material. Effects of preculture time in a medium rich in sucrose and prefreezing treatments (0 °C or/and − 20 °C) on plant recovery were evaluated. Rhizome Buds of the “Morado de Huétor” landrace were incubated in preculture medium (MS + 0.3 M sucrose) for 48 h, encapsulated in alginate beads and desiccated until a water content of 35%, prefrozen for one hour at 0 °C plus one hour at − 20 °C, followed by cryopreservation in liquid nitrogen, and then were rewarmed and recovered in ARBM medium for 6 weeks and finally incubated in ARBM-0 for 4 weeks. Analyses of ploidy and molecular stability of plantlets recovered from cryopreserved rhizome buds of two selected genotypes showed no differences compared with the mother plants. Cryopreservation of RB explants of A. officinalis with this Encapsulation–Dehydration procedure will be useful in long-term preservation programs. © 2018, Springer Science+Business Media B.V., part of Springer Nature. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v133_n3_p395_CarmonaMartin http://hdl.handle.net/20.500.12110/paper_01676857_v133_n3_p395_CarmonaMartin
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Asparagus
Cryopreservation
Encapsulation–dehydration
Flow cytometry
Molecular markers
Rhizome bud
Dehydration
Flow cytometry
Recovery
Sugar (sucrose)
Asparagus
Cryo-preservation
Dehydration procedure
Genetic stability
Long-term preservation
Molecular marker
Molecular stability
Rhizome bud
Biological materials preservation
Buds
Dehydration
Explants
Moisture Content
Peltogyne
Preservation
Recovery
Stability
spellingShingle Asparagus
Cryopreservation
Encapsulation–dehydration
Flow cytometry
Molecular markers
Rhizome bud
Dehydration
Flow cytometry
Recovery
Sugar (sucrose)
Asparagus
Cryo-preservation
Dehydration procedure
Genetic stability
Long-term preservation
Molecular marker
Molecular stability
Rhizome bud
Biological materials preservation
Buds
Dehydration
Explants
Moisture Content
Peltogyne
Preservation
Recovery
Stability
Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability
topic_facet Asparagus
Cryopreservation
Encapsulation–dehydration
Flow cytometry
Molecular markers
Rhizome bud
Dehydration
Flow cytometry
Recovery
Sugar (sucrose)
Asparagus
Cryo-preservation
Dehydration procedure
Genetic stability
Long-term preservation
Molecular marker
Molecular stability
Rhizome bud
Biological materials preservation
Buds
Dehydration
Explants
Moisture Content
Peltogyne
Preservation
Recovery
Stability
description We describe an encapsulation–dehydration procedure with prefreezing steps for the cryopreservation of rhizome bud explants of Asparagus officinalis L. cv. Morado de Huétor. With this procedure, survival of Rhizome buds was at least 84 and 42% developed to complete plantlets at 8 weeks. Flow cytometry and EST-SSR molecular markers were used to assess genetic stability of the regenerated material. Effects of preculture time in a medium rich in sucrose and prefreezing treatments (0 °C or/and − 20 °C) on plant recovery were evaluated. Rhizome Buds of the “Morado de Huétor” landrace were incubated in preculture medium (MS + 0.3 M sucrose) for 48 h, encapsulated in alginate beads and desiccated until a water content of 35%, prefrozen for one hour at 0 °C plus one hour at − 20 °C, followed by cryopreservation in liquid nitrogen, and then were rewarmed and recovered in ARBM medium for 6 weeks and finally incubated in ARBM-0 for 4 weeks. Analyses of ploidy and molecular stability of plantlets recovered from cryopreserved rhizome buds of two selected genotypes showed no differences compared with the mother plants. Cryopreservation of RB explants of A. officinalis with this Encapsulation–Dehydration procedure will be useful in long-term preservation programs. © 2018, Springer Science+Business Media B.V., part of Springer Nature.
title Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability
title_short Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability
title_full Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability
title_fullStr Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability
title_full_unstemmed Cryopreservation of rhizome buds of Asparagus officinalis L. (cv. Morado de Huétor) and evaluation of their genetic stability
title_sort cryopreservation of rhizome buds of asparagus officinalis l. (cv. morado de huétor) and evaluation of their genetic stability
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v133_n3_p395_CarmonaMartin
http://hdl.handle.net/20.500.12110/paper_01676857_v133_n3_p395_CarmonaMartin
_version_ 1768542785264156672

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