Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk

Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the “Mar Menor” lagoon, in Murcia (Spain), and is the only “Critically Endangered” species of the genus Asparagus. Despite being protected, the number of plants has decreased in the las...

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Detalles Bibliográficos
Publicado: 2018
Materias:
Asparagus spp
Endangered species
Flow cytometry
Germplasm preservation
Rhizome buds
Conservation
Sugar (sucrose)
Critically endangered
Extinction risks
Genetic resources
Germplasms
Micropropagated plantlets
Plants (botany)
Biodiversity
Genetic Engineering
Plants
Risk Assessment
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v132_n3_p573_Regalado
http://hdl.handle.net/20.500.12110/paper_01676857_v132_n3_p573_Regalado
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01676857_v132_n3_p573_Regalado
record_format dspace
spelling paper:paper_01676857_v132_n3_p573_Regalado2023-06-08T15:16:44Z Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk Asparagus spp Endangered species Flow cytometry Germplasm preservation Rhizome buds Conservation Flow cytometry Sugar (sucrose) Asparagus spp Critically endangered Endangered species Extinction risks Genetic resources Germplasms Micropropagated plantlets Rhizome buds Plants (botany) Biodiversity Genetic Engineering Plants Risk Assessment Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the “Mar Menor” lagoon, in Murcia (Spain), and is the only “Critically Endangered” species of the genus Asparagus. Despite being protected, the number of plants has decreased in the last years due to the urbanization of its natural habitat. This species is a valuable genetic resource for asparagus breeding because of its special characteristics. So, the development of a micropropagation protocol is crucial to its conservation and use in breeding programs. The micropropagation protocol from asparagus rhizome buds previously developed by our research group has been adapted for A. macrorrhizus. Rhizome buds of A. macrorrhizus were extracted, disinfected, and then cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS medium supplemented with 0.3 mg l− 1 NAA, 0.1 mg l− 1 KIN, 2 mg l− 1 ancymidol and 6% sucrose. A percentage of 69.7 ± 8.0% of the rhizome buds developed shoots, but only 17.4 ± 7.9% of them rooted. To increase this low rooting rate, the shoots were cultured on Macrorrhizus Rooting Media (MRM) supplemented with three different concentrations of IBA. The highest rooting rate (55.0 ± 7.9%) was reached when shoots were incubated in MRM-2 consisting of MS medium supplemented with 2 mg l− 1 IBA and 4% sucrose. The acclimatization rate of the micropropagated plantlets was 90%. The method developed in this study allows the micropropagation of A. macrorrhizus, offering a new option to preserve this almost extinct species. © 2017, Springer Science+Business Media B.V., part of Springer Nature. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v132_n3_p573_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v132_n3_p573_Regalado
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Asparagus spp
Endangered species
Flow cytometry
Germplasm preservation
Rhizome buds
Conservation
Flow cytometry
Sugar (sucrose)
Asparagus spp
Critically endangered
Endangered species
Extinction risks
Genetic resources
Germplasms
Micropropagated plantlets
Rhizome buds
Plants (botany)
Biodiversity
Genetic Engineering
Plants
Risk Assessment
spellingShingle Asparagus spp
Endangered species
Flow cytometry
Germplasm preservation
Rhizome buds
Conservation
Flow cytometry
Sugar (sucrose)
Asparagus spp
Critically endangered
Endangered species
Extinction risks
Genetic resources
Germplasms
Micropropagated plantlets
Rhizome buds
Plants (botany)
Biodiversity
Genetic Engineering
Plants
Risk Assessment
Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
topic_facet Asparagus spp
Endangered species
Flow cytometry
Germplasm preservation
Rhizome buds
Conservation
Flow cytometry
Sugar (sucrose)
Asparagus spp
Critically endangered
Endangered species
Extinction risks
Genetic resources
Germplasms
Micropropagated plantlets
Rhizome buds
Plants (botany)
Biodiversity
Genetic Engineering
Plants
Risk Assessment
description Asparagus macrorrhizus: is a new species, which has been recently described. It is limited to the area surrounding the “Mar Menor” lagoon, in Murcia (Spain), and is the only “Critically Endangered” species of the genus Asparagus. Despite being protected, the number of plants has decreased in the last years due to the urbanization of its natural habitat. This species is a valuable genetic resource for asparagus breeding because of its special characteristics. So, the development of a micropropagation protocol is crucial to its conservation and use in breeding programs. The micropropagation protocol from asparagus rhizome buds previously developed by our research group has been adapted for A. macrorrhizus. Rhizome buds of A. macrorrhizus were extracted, disinfected, and then cultured on Asparagus Rhizome Bud Medium (ARBM) consisting of MS medium supplemented with 0.3 mg l− 1 NAA, 0.1 mg l− 1 KIN, 2 mg l− 1 ancymidol and 6% sucrose. A percentage of 69.7 ± 8.0% of the rhizome buds developed shoots, but only 17.4 ± 7.9% of them rooted. To increase this low rooting rate, the shoots were cultured on Macrorrhizus Rooting Media (MRM) supplemented with three different concentrations of IBA. The highest rooting rate (55.0 ± 7.9%) was reached when shoots were incubated in MRM-2 consisting of MS medium supplemented with 2 mg l− 1 IBA and 4% sucrose. The acclimatization rate of the micropropagated plantlets was 90%. The method developed in this study allows the micropropagation of A. macrorrhizus, offering a new option to preserve this almost extinct species. © 2017, Springer Science+Business Media B.V., part of Springer Nature.
title Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
title_short Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
title_full Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
title_fullStr Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
title_full_unstemmed Micropropagation of Asparagus macrorrhizus, a Spanish endemic species in extreme extinction risk
title_sort micropropagation of asparagus macrorrhizus, a spanish endemic species in extreme extinction risk
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v132_n3_p573_Regalado
http://hdl.handle.net/20.500.12110/paper_01676857_v132_n3_p573_Regalado
_version_ 1768541887146229760

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