Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum

Italian ryegrass (Lolium multiflorum) is an annual grass considered as one of the most important temperate forage grasses in the world. However, it is recalcitrant to plant tissue culture techniques hindering its genetic manipulation. Epichloë occultans is an endophytic fungus associated with L. mul...

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Detalles Bibliográficos
Autores principales:	Novas, Maria Victoria, Iannone, Leopoldo Javier
Publicado:	2017
Materias:	Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Seed Efficiency Fungi Plants Reforestation
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v130_n1_p37_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v130_n1_p37_Regalado
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01676857_v130_n1_p37_Regalado
record_format	dspace
spelling	paper:paper_01676857_v130_n1_p37_Regalado2023-06-08T15:16:44Z Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum Novas, Maria Victoria Iannone, Leopoldo Javier Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Seed Efficiency Fungi Plants Reforestation Italian ryegrass (Lolium multiflorum) is an annual grass considered as one of the most important temperate forage grasses in the world. However, it is recalcitrant to plant tissue culture techniques hindering its genetic manipulation. Epichloë occultans is an endophytic fungus associated with L. multiflorum. This symbiosis causes improvements in physiological and ecological traits of the host plants. The objective of this work was to study the effect of E. occultans on L. multiflorum micropropagation. We compared the response of endophyte-infected (E+) and endophyte-free (E−) seeds in different micropropagation stages. The E+ seeds were more successful than E− seeds in in vitro germination (83 ± 5 vs. 63 ± 6%), callus induction (78 ± 5 vs. 57 ± 6%), callus proliferation (average diameter of 21.5 ± 1.3 mm in two subcultures vs 17.3 ± 0.8 mm in three subcultures) and plant regeneration from callus (83 ± 7 vs. 30 ± 8%). These results indicate that E. occultans enhances significantly L. multiflorum micropropagation. The use of endophyte-infected (E+) seeds can be a solution to make this grass more amenable to different biotechnological tools, such as the genetic transformation. © 2017, Springer Science+Business Media Dordrecht. Fil:Novas, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iannone, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2017 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v130_n1_p37_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v130_n1_p37_Regalado
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Seed Efficiency Fungi Plants Reforestation
spellingShingle	Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Seed Efficiency Fungi Plants Reforestation Novas, Maria Victoria Iannone, Leopoldo Javier Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
topic_facet	Callus culture Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Nucleic acids Plants (botany) Tissue culture Callus cultures Fungal endophytes Grasses Italian ryegrass Organogenesis Plant regeneration Seed Efficiency Fungi Plants Reforestation
description	Italian ryegrass (Lolium multiflorum) is an annual grass considered as one of the most important temperate forage grasses in the world. However, it is recalcitrant to plant tissue culture techniques hindering its genetic manipulation. Epichloë occultans is an endophytic fungus associated with L. multiflorum. This symbiosis causes improvements in physiological and ecological traits of the host plants. The objective of this work was to study the effect of E. occultans on L. multiflorum micropropagation. We compared the response of endophyte-infected (E+) and endophyte-free (E−) seeds in different micropropagation stages. The E+ seeds were more successful than E− seeds in in vitro germination (83 ± 5 vs. 63 ± 6%), callus induction (78 ± 5 vs. 57 ± 6%), callus proliferation (average diameter of 21.5 ± 1.3 mm in two subcultures vs 17.3 ± 0.8 mm in three subcultures) and plant regeneration from callus (83 ± 7 vs. 30 ± 8%). These results indicate that E. occultans enhances significantly L. multiflorum micropropagation. The use of endophyte-infected (E+) seeds can be a solution to make this grass more amenable to different biotechnological tools, such as the genetic transformation. © 2017, Springer Science+Business Media Dordrecht.
author	Novas, Maria Victoria Iannone, Leopoldo Javier
author_facet	Novas, Maria Victoria Iannone, Leopoldo Javier
author_sort	Novas, Maria Victoria
title	Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
title_short	Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
title_full	Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
title_fullStr	Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
title_full_unstemmed	Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum
title_sort	epichloë occultans enhances micropropagation efficiency in lolium multiflorum
publishDate	2017
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676857_v130_n1_p37_Regalado http://hdl.handle.net/20.500.12110/paper_01676857_v130_n1_p37_Regalado
work_keys_str_mv	AT novasmariavictoria epichloeoccultansenhancesmicropropagationefficiencyinloliummultiflorum AT iannoneleopoldojavier epichloeoccultansenhancesmicropropagationefficiencyinloliummultiflorum
_version_	1768545643296456704

Epichloë occultans enhances micropropagation efficiency in Lolium multiflorum

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