Bone marrow fibroblastic progenitors in patients with advanced breast cancer
Bone marrow fibroblast colony-forming cells (CFU-F) were studied in fifteen consecutive untreated breast cancer patients (BCP) with clinical stages III and IV, and in sixteen normal controls (NC). A decreased number of CFU-F was observed in BCP compared to NC (p < 0.004). Confluence of the ad...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v45_n3_p211_Chasseing http://hdl.handle.net/20.500.12110/paper_01676806_v45_n3_p211_Chasseing |
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paper:paper_01676806_v45_n3_p211_Chasseing2023-06-08T15:16:43Z Bone marrow fibroblastic progenitors in patients with advanced breast cancer Fibroblasts and interleukin-β indometacin interleukin 1beta muramyl dipeptide prostaglandin E2 trypsin adherent cell advanced cancer article bone marrow biopsy bone marrow cell breast cancer cancer staging cell culture cell stimulation clinical article colony forming cell controlled study enzyme linked immunosorbent assay female fibroblast fibroblast culture human human cell mononuclear cell priority journal prostaglandin release stem cell supernatant Biopsy, Needle Bone Marrow Cells Breast Breast Neoplasms Cells, Cultured Dinoprostone Female Fibroblasts Humans Interleukin-1 Stem Cells Bone marrow fibroblast colony-forming cells (CFU-F) were studied in fifteen consecutive untreated breast cancer patients (BCP) with clinical stages III and IV, and in sixteen normal controls (NC). A decreased number of CFU-F was observed in BCP compared to NC (p < 0.004). Confluence of the adherent cell layer was observed in all normal bone marrow mononuclear cells (MC) cultures, while a lower proportion of cultures from BCP (11/15) showed confluent adherent cell layers. When MC cultures of BCP were treated with indomethacin (Indo, 10 -6 M) 50% of them increased the number of CFU-F compared to the value obtained without treatment. In addition, a significant increase in the release of PGE2 in BCP cultures was observed before Indo treatment. Moreover, after MC were fractionated into adherent and non-adherent progenitors, the CFU-F decreased in both types of fractions of BCP compared to NC value (p < 0.02 and < 0.05, respectively). The number of light density MC per 10 ml of bone marrow aspirate and the number of trypsin-sensitive adherent progenitors were lower than NC in BCP (p < 0.02 and 0.013, respectively). Total MC and fibroblasts (fourth passage) were cultivated to evaluate the production of interleukin-1β (IL-1β) by ELISA methodology. Results indicated no difference of IL-1β spontaneous release when total MC cultures of both groups were compared. However, the levels of this cytokine were lower (< 10 pg/ml) in fibroblast culture supernatants of BCP compared to NC (1217 ± 74 pg/ml). Fibroblast cultures from BCP showed low or no release of IL-1β after muramyl-dipeptide (MDP, 1 μg/ml) stimulation. In conclusion, the defective proliferative and confluence capacity of BCP fibroblastic progenitors may be related to the decrease in the production of IL-1β by these precursors. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v45_n3_p211_Chasseing http://hdl.handle.net/20.500.12110/paper_01676806_v45_n3_p211_Chasseing |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fibroblasts and interleukin-β indometacin interleukin 1beta muramyl dipeptide prostaglandin E2 trypsin adherent cell advanced cancer article bone marrow biopsy bone marrow cell breast cancer cancer staging cell culture cell stimulation clinical article colony forming cell controlled study enzyme linked immunosorbent assay female fibroblast fibroblast culture human human cell mononuclear cell priority journal prostaglandin release stem cell supernatant Biopsy, Needle Bone Marrow Cells Breast Breast Neoplasms Cells, Cultured Dinoprostone Female Fibroblasts Humans Interleukin-1 Stem Cells |
| spellingShingle |
Fibroblasts and interleukin-β indometacin interleukin 1beta muramyl dipeptide prostaglandin E2 trypsin adherent cell advanced cancer article bone marrow biopsy bone marrow cell breast cancer cancer staging cell culture cell stimulation clinical article colony forming cell controlled study enzyme linked immunosorbent assay female fibroblast fibroblast culture human human cell mononuclear cell priority journal prostaglandin release stem cell supernatant Biopsy, Needle Bone Marrow Cells Breast Breast Neoplasms Cells, Cultured Dinoprostone Female Fibroblasts Humans Interleukin-1 Stem Cells Bone marrow fibroblastic progenitors in patients with advanced breast cancer |
| topic_facet |
Fibroblasts and interleukin-β indometacin interleukin 1beta muramyl dipeptide prostaglandin E2 trypsin adherent cell advanced cancer article bone marrow biopsy bone marrow cell breast cancer cancer staging cell culture cell stimulation clinical article colony forming cell controlled study enzyme linked immunosorbent assay female fibroblast fibroblast culture human human cell mononuclear cell priority journal prostaglandin release stem cell supernatant Biopsy, Needle Bone Marrow Cells Breast Breast Neoplasms Cells, Cultured Dinoprostone Female Fibroblasts Humans Interleukin-1 Stem Cells |
| description |
Bone marrow fibroblast colony-forming cells (CFU-F) were studied in fifteen consecutive untreated breast cancer patients (BCP) with clinical stages III and IV, and in sixteen normal controls (NC). A decreased number of CFU-F was observed in BCP compared to NC (p < 0.004). Confluence of the adherent cell layer was observed in all normal bone marrow mononuclear cells (MC) cultures, while a lower proportion of cultures from BCP (11/15) showed confluent adherent cell layers. When MC cultures of BCP were treated with indomethacin (Indo, 10 -6 M) 50% of them increased the number of CFU-F compared to the value obtained without treatment. In addition, a significant increase in the release of PGE2 in BCP cultures was observed before Indo treatment. Moreover, after MC were fractionated into adherent and non-adherent progenitors, the CFU-F decreased in both types of fractions of BCP compared to NC value (p < 0.02 and < 0.05, respectively). The number of light density MC per 10 ml of bone marrow aspirate and the number of trypsin-sensitive adherent progenitors were lower than NC in BCP (p < 0.02 and 0.013, respectively). Total MC and fibroblasts (fourth passage) were cultivated to evaluate the production of interleukin-1β (IL-1β) by ELISA methodology. Results indicated no difference of IL-1β spontaneous release when total MC cultures of both groups were compared. However, the levels of this cytokine were lower (< 10 pg/ml) in fibroblast culture supernatants of BCP compared to NC (1217 ± 74 pg/ml). Fibroblast cultures from BCP showed low or no release of IL-1β after muramyl-dipeptide (MDP, 1 μg/ml) stimulation. In conclusion, the defective proliferative and confluence capacity of BCP fibroblastic progenitors may be related to the decrease in the production of IL-1β by these precursors. |
| title |
Bone marrow fibroblastic progenitors in patients with advanced breast cancer |
| title_short |
Bone marrow fibroblastic progenitors in patients with advanced breast cancer |
| title_full |
Bone marrow fibroblastic progenitors in patients with advanced breast cancer |
| title_fullStr |
Bone marrow fibroblastic progenitors in patients with advanced breast cancer |
| title_full_unstemmed |
Bone marrow fibroblastic progenitors in patients with advanced breast cancer |
| title_sort |
bone marrow fibroblastic progenitors in patients with advanced breast cancer |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v45_n3_p211_Chasseing http://hdl.handle.net/20.500.12110/paper_01676806_v45_n3_p211_Chasseing |
| _version_ |
1768543699232358400 |