Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells

Human breast tumor cells MCF-7 were grown during 5 days in the presence of Adriamycin and the IC50 was 50 nM with the highest sublethal concentration 0.1 μM. At this latter concentration Adriamycin produced a complete inhibition of cell division and a partial reversion to a normal breast epithelial...

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Detalles Bibliográficos
Publicado:	1990
Materias:	Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v17_n2_p145_Bustamante http://hdl.handle.net/20.500.12110/paper_01676806_v17_n2_p145_Bustamante
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01676806_v17_n2_p145_Bustamante
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spelling	paper:paper_01676806_v17_n2_p145_Bustamante2023-06-08T15:16:42Z Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured Human breast tumor cells MCF-7 were grown during 5 days in the presence of Adriamycin and the IC50 was 50 nM with the highest sublethal concentration 0.1 μM. At this latter concentration Adriamycin produced a complete inhibition of cell division and a partial reversion to a normal breast epithelial appearance. Similar effects of Adriamycin were observed in cells cultured in the presence of 10% FBS and in a chemically defined medium, with Se-glutathione peroxidase activities of 3.8 and 1.3 U/mg of protein, respectively. Cell size and cell oxygen uptake were increased by 41% and by 50%, respectively, in Adriamycin-treated cells. The spontaneous chemiluminescence of monolayers of intact MCF-7 cells (81 ± 9 cps/mg protein) was increased by 48% in the Adriamycin-treated cultures (120 ± 11 cps/mg of protein) in agreement with a 91% higher concentration of malondialdehyde in the same cultures. Adriamycin treatment produced a 71% increase in the steady state concentration of H202, which was estimated assuming diffusion equilibrium with the external medium, from 1.38 μM in the control cells to 2.38 μM in the treated cells. Cyanide-insensitive respiration was also higher in the cells exposed to the drug than in the control cells. Adriamycin did not affect the activity of the antioxidant enzymes, Cu-Zn and Mn-superoxide dismutase, Se and non-Se-glutathione peroxidase, and catalase. These results contribute to the current hypothesis that oxygen free radicals produced by Adriamycin redox cycling are responsible for at least part of the cytotoxic effects due to this drug. © 1990 Kluwer Academic Publishers. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v17_n2_p145_Bustamante http://hdl.handle.net/20.500.12110/paper_01676806_v17_n2_p145_Bustamante
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured
spellingShingle	Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells
topic_facet	Adriamycin antioxidant enzymes chemiluminescence free radicals MCF-7 breast tumor cells oxy-radical formation peroxides doxorubicin hydroperoxide article breast cancer cancer cell culture cell growth female human human tissue Analysis of Variance Animal Cell Division Doxorubicin Free Radicals Human Hydrogen Peroxide Lipid Peroxidation Luminescence Mammary Neoplasms, Experimental Oxygen Consumption Tumor Cells, Cultured
description	Human breast tumor cells MCF-7 were grown during 5 days in the presence of Adriamycin and the IC50 was 50 nM with the highest sublethal concentration 0.1 μM. At this latter concentration Adriamycin produced a complete inhibition of cell division and a partial reversion to a normal breast epithelial appearance. Similar effects of Adriamycin were observed in cells cultured in the presence of 10% FBS and in a chemically defined medium, with Se-glutathione peroxidase activities of 3.8 and 1.3 U/mg of protein, respectively. Cell size and cell oxygen uptake were increased by 41% and by 50%, respectively, in Adriamycin-treated cells. The spontaneous chemiluminescence of monolayers of intact MCF-7 cells (81 ± 9 cps/mg protein) was increased by 48% in the Adriamycin-treated cultures (120 ± 11 cps/mg of protein) in agreement with a 91% higher concentration of malondialdehyde in the same cultures. Adriamycin treatment produced a 71% increase in the steady state concentration of H202, which was estimated assuming diffusion equilibrium with the external medium, from 1.38 μM in the control cells to 2.38 μM in the treated cells. Cyanide-insensitive respiration was also higher in the cells exposed to the drug than in the control cells. Adriamycin did not affect the activity of the antioxidant enzymes, Cu-Zn and Mn-superoxide dismutase, Se and non-Se-glutathione peroxidase, and catalase. These results contribute to the current hypothesis that oxygen free radicals produced by Adriamycin redox cycling are responsible for at least part of the cytotoxic effects due to this drug. © 1990 Kluwer Academic Publishers.
title	Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells
title_short	Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells
title_full	Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells
title_fullStr	Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells
title_full_unstemmed	Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells
title_sort	adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells
publishDate	1990
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v17_n2_p145_Bustamante http://hdl.handle.net/20.500.12110/paper_01676806_v17_n2_p145_Bustamante
_version_	1768546671822635008

Adriamycin effects on hydroperoxide metabolism and growth of human breast tumor cells

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