Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein

Tristetraprolin (TTP) is a RNA-binding protein that inhibits the expression of pro-inflammatory cytokines and invasiveness-associated genes. TTP levels are decreased in many different cancer types and it has been proposed that this protein could be used as a prognostic factor in breast cancer. Here,...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Goddio, Maria Victoria, Slomiansky, Victoria, Tocci, Johanna Melisa, Kordon, Edith Claudia
Publicado: 2012
Materias:
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v135_n3_p749_Goddio
http://hdl.handle.net/20.500.12110/paper_01676806_v135_n3_p749_Goddio
Aporte de:
id paper:paper_01676806_v135_n3_p749_Goddio
record_format dspace
spelling paper:paper_01676806_v135_n3_p749_Goddio2023-06-08T15:16:41Z Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein Goddio, Maria Victoria Slomiansky, Victoria Tocci, Johanna Melisa Kordon, Edith Claudia Breast cancer Lactation Mammary gland Prolactin Tristetraprolin messenger RNA prolactin RNA binding protein STAT5a protein tristetraprolin 3' untranslated region animal tissue article AU rich element breast cancer breast carcinoma breast cell breast lesion breast tumor cancer cell cancer grading cancer invasion cell proliferation controlled study DNA microarray gene expression human human tissue immunohistochemistry lactation mammary gland mouse nonhuman priority journal protein expression reverse transcription polymerase chain reaction serial analysis of gene expression tissue differentiation Animals Base Sequence Breast Neoplasms Cell Differentiation Cell Line, Tumor Female Gene Expression Regulation, Neoplastic Humans Lactation Mammary Glands, Animal Mammary Glands, Human Mammary Neoplasms, Animal Mice Mice, Inbred BALB C Molecular Sequence Data Pregnancy Prolactin STAT5 Transcription Factor Tristetraprolin Tumor Suppressor Proteins Tristetraprolin (TTP) is a RNA-binding protein that inhibits the expression of pro-inflammatory cytokines and invasiveness-associated genes. TTP levels are decreased in many different cancer types and it has been proposed that this protein could be used as a prognostic factor in breast cancer. Here, using publicly available DNA microarray datasets, "serial analysis of gene expression" libraries and qRT-PCR analysis, we determined that TTP mRNA is present in normal breast cells and its levels are significantly decreased in all breast cancer subtypes. In addition, by immunostaining, we found that TTP expression is higher in normal breast tissue and benign lesions than in infiltrating carcinomas. Among these, lower grade tumors showed increased TTP expression compared to higher grade cancers. Therefore, these data indicate that TTP protein levels would provide a better negative correlation with breast cancer invasiveness than TTP transcript levels. In mice, we found that TTP mRNA and protein expression is also diminished in mammary tumors. Interestingly, a strong positive association of TTP expression and mammary differentiation was identified in normal and tumor cells. In fact, TTP expression is highly increased during lactation, showing good correlation with various mammary differentiation factors. TTP expression was also induced in mammary HC11 cells treated with lactogenic hormones, mainly by prolactin, through Stat5A activation. The effect of this hormone was highly dependent on mammary differentiation status, as prolactin was unable to elicit a similar response in proliferating or neoplastic mammary cells. In summary, these studies show that TTP expression is strongly linked to the mammary differentiation program in human and mice, suggesting that this protein might play specific and relevant roles in the normal physiology of the gland. © Springer Science+Business Media, LLC. 2012. Fil:Goddio, M.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Slomiansky, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tocci, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordon, E.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v135_n3_p749_Goddio http://hdl.handle.net/20.500.12110/paper_01676806_v135_n3_p749_Goddio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Breast cancer
Lactation
Mammary gland
Prolactin
Tristetraprolin
messenger RNA
prolactin
RNA binding protein
STAT5a protein
tristetraprolin
3' untranslated region
animal tissue
article
AU rich element
breast cancer
breast carcinoma
breast cell
breast lesion
breast tumor
cancer cell
cancer grading
cancer invasion
cell proliferation
controlled study
DNA microarray
gene expression
human
human tissue
immunohistochemistry
lactation
mammary gland
mouse
nonhuman
priority journal
protein expression
reverse transcription polymerase chain reaction
serial analysis of gene expression
tissue differentiation
Animals
Base Sequence
Breast Neoplasms
Cell Differentiation
Cell Line, Tumor
Female
Gene Expression Regulation, Neoplastic
Humans
Lactation
Mammary Glands, Animal
Mammary Glands, Human
Mammary Neoplasms, Animal
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Pregnancy
Prolactin
STAT5 Transcription Factor
Tristetraprolin
Tumor Suppressor Proteins
spellingShingle Breast cancer
Lactation
Mammary gland
Prolactin
Tristetraprolin
messenger RNA
prolactin
RNA binding protein
STAT5a protein
tristetraprolin
3' untranslated region
animal tissue
article
AU rich element
breast cancer
breast carcinoma
breast cell
breast lesion
breast tumor
cancer cell
cancer grading
cancer invasion
cell proliferation
controlled study
DNA microarray
gene expression
human
human tissue
immunohistochemistry
lactation
mammary gland
mouse
nonhuman
priority journal
protein expression
reverse transcription polymerase chain reaction
serial analysis of gene expression
tissue differentiation
Animals
Base Sequence
Breast Neoplasms
Cell Differentiation
Cell Line, Tumor
Female
Gene Expression Regulation, Neoplastic
Humans
Lactation
Mammary Glands, Animal
Mammary Glands, Human
Mammary Neoplasms, Animal
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Pregnancy
Prolactin
STAT5 Transcription Factor
Tristetraprolin
Tumor Suppressor Proteins
Goddio, Maria Victoria
Slomiansky, Victoria
Tocci, Johanna Melisa
Kordon, Edith Claudia
Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein
topic_facet Breast cancer
Lactation
Mammary gland
Prolactin
Tristetraprolin
messenger RNA
prolactin
RNA binding protein
STAT5a protein
tristetraprolin
3' untranslated region
animal tissue
article
AU rich element
breast cancer
breast carcinoma
breast cell
breast lesion
breast tumor
cancer cell
cancer grading
cancer invasion
cell proliferation
controlled study
DNA microarray
gene expression
human
human tissue
immunohistochemistry
lactation
mammary gland
mouse
nonhuman
priority journal
protein expression
reverse transcription polymerase chain reaction
serial analysis of gene expression
tissue differentiation
Animals
Base Sequence
Breast Neoplasms
Cell Differentiation
Cell Line, Tumor
Female
Gene Expression Regulation, Neoplastic
Humans
Lactation
Mammary Glands, Animal
Mammary Glands, Human
Mammary Neoplasms, Animal
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Pregnancy
Prolactin
STAT5 Transcription Factor
Tristetraprolin
Tumor Suppressor Proteins
description Tristetraprolin (TTP) is a RNA-binding protein that inhibits the expression of pro-inflammatory cytokines and invasiveness-associated genes. TTP levels are decreased in many different cancer types and it has been proposed that this protein could be used as a prognostic factor in breast cancer. Here, using publicly available DNA microarray datasets, "serial analysis of gene expression" libraries and qRT-PCR analysis, we determined that TTP mRNA is present in normal breast cells and its levels are significantly decreased in all breast cancer subtypes. In addition, by immunostaining, we found that TTP expression is higher in normal breast tissue and benign lesions than in infiltrating carcinomas. Among these, lower grade tumors showed increased TTP expression compared to higher grade cancers. Therefore, these data indicate that TTP protein levels would provide a better negative correlation with breast cancer invasiveness than TTP transcript levels. In mice, we found that TTP mRNA and protein expression is also diminished in mammary tumors. Interestingly, a strong positive association of TTP expression and mammary differentiation was identified in normal and tumor cells. In fact, TTP expression is highly increased during lactation, showing good correlation with various mammary differentiation factors. TTP expression was also induced in mammary HC11 cells treated with lactogenic hormones, mainly by prolactin, through Stat5A activation. The effect of this hormone was highly dependent on mammary differentiation status, as prolactin was unable to elicit a similar response in proliferating or neoplastic mammary cells. In summary, these studies show that TTP expression is strongly linked to the mammary differentiation program in human and mice, suggesting that this protein might play specific and relevant roles in the normal physiology of the gland. © Springer Science+Business Media, LLC. 2012.
author Goddio, Maria Victoria
Slomiansky, Victoria
Tocci, Johanna Melisa
Kordon, Edith Claudia
author_facet Goddio, Maria Victoria
Slomiansky, Victoria
Tocci, Johanna Melisa
Kordon, Edith Claudia
author_sort Goddio, Maria Victoria
title Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein
title_short Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein
title_full Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein
title_fullStr Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein
title_full_unstemmed Mammary differentiation induces expression of Tristetraprolin, a tumor suppressor AU-rich mRNA-binding protein
title_sort mammary differentiation induces expression of tristetraprolin, a tumor suppressor au-rich mrna-binding protein
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01676806_v135_n3_p749_Goddio
http://hdl.handle.net/20.500.12110/paper_01676806_v135_n3_p749_Goddio
work_keys_str_mv AT goddiomariavictoria mammarydifferentiationinducesexpressionoftristetraprolinatumorsuppressoraurichmrnabindingprotein
AT slomianskyvictoria mammarydifferentiationinducesexpressionoftristetraprolinatumorsuppressoraurichmrnabindingprotein
AT toccijohannamelisa mammarydifferentiationinducesexpressionoftristetraprolinatumorsuppressoraurichmrnabindingprotein
AT kordonedithclaudia mammarydifferentiationinducesexpressionoftristetraprolinatumorsuppressoraurichmrnabindingprotein
_version_ 1768542833583587328