Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide

The present work shows that allylisopropylacetamide exerts an inducing effect on δ-aminolevulinic acid synthase and ferrochelatase activities in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the inducing effect produced in both enzymes. Glucose inhibits the induction o...

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Autores principales:	Cánepa, Eduardo Tomás, Llambías, Elena Blanca Cecilia, Grinstein, Moisés
Publicado:	1984
Materias:	(Rat hepatocyte) Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase allylisopropylacetamide bucladesine cycloheximide deoxyglucose ferrochelatase fructose galactose glucose glycerol lactic acid mannose porphobilinogen synthase pyruvic acid alkylisopropylacetamide drug efficacy enzyme induction in vitro study liver liver cell nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Carbohydrates Cycloheximide Enzyme Induction Gene Expression Regulation Heme Liver Lyases Male Rats Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v804_n1_p8_Canepa http://hdl.handle.net/20.500.12110/paper_01674889_v804_n1_p8_Canepa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01674889_v804_n1_p8_Canepa
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spelling	paper:paper_01674889_v804_n1_p8_Canepa2023-06-08T15:16:30Z Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide Cánepa, Eduardo Tomás Llambías, Elena Blanca Cecilia Grinstein, Moisés (Rat hepatocyte) Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase allylisopropylacetamide bucladesine cycloheximide deoxyglucose ferrochelatase fructose galactose glucose glycerol lactic acid mannose porphobilinogen synthase pyruvic acid alkylisopropylacetamide drug efficacy enzyme induction in vitro study liver liver cell nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Carbohydrates Cycloheximide Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Heme Liver Lyases Male Rats Support, Non-U.S. Gov't The present work shows that allylisopropylacetamide exerts an inducing effect on δ-aminolevulinic acid synthase and ferrochelatase activities in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the inducing effect produced in both enzymes. Glucose inhibits the induction of δ-aminolevulinic acid synthase and ferrochelatase in this in vitro system. A similar effect was observed with fructose and 2-deoxyglucose. No glucose effect was observed with galactose, mannose, glycerol, pyruvate and lactate. The glucose effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. The simple in vitro method used in the present work promises to be a very useful tool for studies of regulatory mechanisms of porphyrin and heme biosynthesis in hepatocytes under normal and pathological conditions (hepatic porphyrias). © 1984. Fil:Canepa, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambias, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1984 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v804_n1_p8_Canepa http://hdl.handle.net/20.500.12110/paper_01674889_v804_n1_p8_Canepa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	(Rat hepatocyte) Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase allylisopropylacetamide bucladesine cycloheximide deoxyglucose ferrochelatase fructose galactose glucose glycerol lactic acid mannose porphobilinogen synthase pyruvic acid alkylisopropylacetamide drug efficacy enzyme induction in vitro study liver liver cell nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Carbohydrates Cycloheximide Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Heme Liver Lyases Male Rats Support, Non-U.S. Gov't
spellingShingle	(Rat hepatocyte) Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase allylisopropylacetamide bucladesine cycloheximide deoxyglucose ferrochelatase fructose galactose glucose glycerol lactic acid mannose porphobilinogen synthase pyruvic acid alkylisopropylacetamide drug efficacy enzyme induction in vitro study liver liver cell nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Carbohydrates Cycloheximide Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Heme Liver Lyases Male Rats Support, Non-U.S. Gov't Cánepa, Eduardo Tomás Llambías, Elena Blanca Cecilia Grinstein, Moisés Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide
topic_facet	(Rat hepatocyte) Enzyme induction Ferrochelatase Glucose δ-Aminolevulinate synthase allylisopropylacetamide bucladesine cycloheximide deoxyglucose ferrochelatase fructose galactose glucose glycerol lactic acid mannose porphobilinogen synthase pyruvic acid alkylisopropylacetamide drug efficacy enzyme induction in vitro study liver liver cell nonhuman rat 5-Aminolevulinate Synthetase Allylisopropylacetamide Animal Bucladesine Carbohydrates Cycloheximide Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Heme Liver Lyases Male Rats Support, Non-U.S. Gov't
description	The present work shows that allylisopropylacetamide exerts an inducing effect on δ-aminolevulinic acid synthase and ferrochelatase activities in isolated rat hepatocytes of normal adult rats. Dibutyryl cyclic AMP enhances the inducing effect produced in both enzymes. Glucose inhibits the induction of δ-aminolevulinic acid synthase and ferrochelatase in this in vitro system. A similar effect was observed with fructose and 2-deoxyglucose. No glucose effect was observed with galactose, mannose, glycerol, pyruvate and lactate. The glucose effect can be reversed with increasing concentrations of dibutyryl cyclic AMP. The simple in vitro method used in the present work promises to be a very useful tool for studies of regulatory mechanisms of porphyrin and heme biosynthesis in hepatocytes under normal and pathological conditions (hepatic porphyrias). © 1984.
author	Cánepa, Eduardo Tomás Llambías, Elena Blanca Cecilia Grinstein, Moisés
author_facet	Cánepa, Eduardo Tomás Llambías, Elena Blanca Cecilia Grinstein, Moisés
author_sort	Cánepa, Eduardo Tomás
title	Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide
title_short	Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide
title_full	Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide
title_fullStr	Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide
title_full_unstemmed	Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide
title_sort	effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide
publishDate	1984
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674889_v804_n1_p8_Canepa http://hdl.handle.net/20.500.12110/paper_01674889_v804_n1_p8_Canepa
work_keys_str_mv	AT canepaeduardotomas effectofglucoseontheinductionofdaminolevulinicacidsynthaseandferrochelataseinisolatedrathepatocytesbyallylisopropylacetamide AT llambiaselenablancacecilia effectofglucoseontheinductionofdaminolevulinicacidsynthaseandferrochelataseinisolatedrathepatocytesbyallylisopropylacetamide AT grinsteinmoises effectofglucoseontheinductionofdaminolevulinicacidsynthaseandferrochelataseinisolatedrathepatocytesbyallylisopropylacetamide
_version_	1768546671637037056

Effect of glucose on the induction of δ-aminolevulinic acid synthase and ferrochelatase in isolated rat hepatocytes by allylisopropylacetamide

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