Polyamines modulate streptomycin-induced mistranslation in Escherichia coli

The effects of intracellular levels of polyamines on both the in vivo inhibition of protein synthesis and the decrease of translation accuracy induced by streptomycin have been studied in polyamine-auxotrophic strains of Escherichia coli infected with the MS2 bacteriophage. The amount of viral coat...

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Autores principales:	Nastri, Horacio Gabriel, Fastame, Inés Gabriela, Algranati, Israel David
Publicado:	1993
Materias:	(E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Support, Non-U.S. Gov't Translation, Genetic
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674781_v1216_n3_p455_Nastri http://hdl.handle.net/20.500.12110/paper_01674781_v1216_n3_p455_Nastri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01674781_v1216_n3_p455_Nastri
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spelling	paper:paper_01674781_v1216_n3_p455_Nastri2023-06-08T15:16:23Z Polyamines modulate streptomycin-induced mistranslation in Escherichia coli Nastri, Horacio Gabriel Fastame, Inés Gabriela Algranati, Israel David (E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Streptomycin Support, Non-U.S. Gov't Translation, Genetic Escherichia coli The effects of intracellular levels of polyamines on both the in vivo inhibition of protein synthesis and the decrease of translation accuracy induced by streptomycin have been studied in polyamine-auxotrophic strains of Escherichia coli infected with the MS2 bacteriophage. The amount of viral coat protein formed was strongly reduced upon addition of increasing concentrations of streptomycin to polyamine-supplemented bacteria. In contrast, the antibiotic almost did not inhibit coat protein synthesis in polyamine-starved cells. The increase of mistranslation frequency elicited by streptomycin was only observed in bacteria grown with putrescine. In these cells several coat protein-satellites were detected after two-dimensional gel electrophoresis. These proteins, more basic than the normal MS2 coat protein, contain multiple substitutions of lysine for asparagine. © 1993. Fil:Nastri, H.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fastame, I.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674781_v1216_n3_p455_Nastri http://hdl.handle.net/20.500.12110/paper_01674781_v1216_n3_p455_Nastri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	(E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Streptomycin Support, Non-U.S. Gov't Translation, Genetic Escherichia coli
spellingShingle	(E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Streptomycin Support, Non-U.S. Gov't Translation, Genetic Escherichia coli Nastri, Horacio Gabriel Fastame, Inés Gabriela Algranati, Israel David Polyamines modulate streptomycin-induced mistranslation in Escherichia coli
topic_facet	(E. coli) Polyamine Streptomycin Translation fidelity coat protein polyamine streptomycin article bacteriophage controlled study escherichia coli nonhuman priority journal rna translation virus infection Autoradiography Bacterial Proteins Capsid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Escherichia coli Levivirus Methionine Molecular Weight Polyamines Streptomycin Support, Non-U.S. Gov't Translation, Genetic Escherichia coli
description	The effects of intracellular levels of polyamines on both the in vivo inhibition of protein synthesis and the decrease of translation accuracy induced by streptomycin have been studied in polyamine-auxotrophic strains of Escherichia coli infected with the MS2 bacteriophage. The amount of viral coat protein formed was strongly reduced upon addition of increasing concentrations of streptomycin to polyamine-supplemented bacteria. In contrast, the antibiotic almost did not inhibit coat protein synthesis in polyamine-starved cells. The increase of mistranslation frequency elicited by streptomycin was only observed in bacteria grown with putrescine. In these cells several coat protein-satellites were detected after two-dimensional gel electrophoresis. These proteins, more basic than the normal MS2 coat protein, contain multiple substitutions of lysine for asparagine. © 1993.
author	Nastri, Horacio Gabriel Fastame, Inés Gabriela Algranati, Israel David
author_facet	Nastri, Horacio Gabriel Fastame, Inés Gabriela Algranati, Israel David
author_sort	Nastri, Horacio Gabriel
title	Polyamines modulate streptomycin-induced mistranslation in Escherichia coli
title_short	Polyamines modulate streptomycin-induced mistranslation in Escherichia coli
title_full	Polyamines modulate streptomycin-induced mistranslation in Escherichia coli
title_fullStr	Polyamines modulate streptomycin-induced mistranslation in Escherichia coli
title_full_unstemmed	Polyamines modulate streptomycin-induced mistranslation in Escherichia coli
title_sort	polyamines modulate streptomycin-induced mistranslation in escherichia coli
publishDate	1993
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01674781_v1216_n3_p455_Nastri http://hdl.handle.net/20.500.12110/paper_01674781_v1216_n3_p455_Nastri
work_keys_str_mv	AT nastrihoraciogabriel polyaminesmodulatestreptomycininducedmistranslationinescherichiacoli AT fastameinesgabriela polyaminesmodulatestreptomycininducedmistranslationinescherichiacoli AT algranatiisraeldavid polyaminesmodulatestreptomycininducedmistranslationinescherichiacoli
_version_	1768541605142200320

Polyamines modulate streptomycin-induced mistranslation in Escherichia coli

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