Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins

Two cdc2-related protein kinases (crk), tzcrk3 and tzcrk1, from the protozoan parasite Trypanosoma cruzi were cloned. tzcrk3 encodes a 35 kDa protein sharing 51.5% amino acid identity with human cdc2 and 82% identity with Trypanosoma brucei CRK3. tzcrk1 encodes a 33 kDa protein sharing 52.7% identit...

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Autores principales:	Gómez, Eliana Beatriz, Kornblihtt, Alberto Rodolfo
Publicado:	1998
Materias:	cdc2-related protein kinase Cell cycle control Mammalian cyclins Trypanosoma cruzi cycline protein kinase amino acid sequence article cell cycle enzyme analysis gene sequence immunoblotting molecular cloning nonhuman nucleotide sequence priority journal protein analysis trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Blotting, Southern CDC2 Protein Kinase Cloning, Molecular COS Cells Cyclins Genes, Protozoan Genetic Complementation Test Histones Humans Molecular Probe Techniques Molecular Sequence Data Protein Kinases Protozoan Proteins Recombinant Proteins Retinoblastoma Protein Sequence Alignment Transfection Leishmania mexicana Mammalia Protozoa Trypanosoma Trypanosoma brucei Trypanosoma congolense
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v91_n2_p337_Gomez http://hdl.handle.net/20.500.12110/paper_01666851_v91_n2_p337_Gomez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_01666851_v91_n2_p337_Gomez2023-06-08T15:16:09Z Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins Gómez, Eliana Beatriz Kornblihtt, Alberto Rodolfo cdc2-related protein kinase Cell cycle control Mammalian cyclins Trypanosoma cruzi cycline protein kinase amino acid sequence article cell cycle enzyme analysis gene sequence immunoblotting molecular cloning nonhuman nucleotide sequence priority journal protein analysis trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Blotting, Southern CDC2 Protein Kinase Cloning, Molecular COS Cells Cyclins Genes, Protozoan Genetic Complementation Test Histones Humans Molecular Probe Techniques Molecular Sequence Data Protein Kinases Protozoan Proteins Recombinant Proteins Retinoblastoma Protein Sequence Alignment Transfection Trypanosoma cruzi Leishmania mexicana Mammalia Protozoa Trypanosoma Trypanosoma brucei Trypanosoma congolense Trypanosoma cruzi Two cdc2-related protein kinases (crk), tzcrk3 and tzcrk1, from the protozoan parasite Trypanosoma cruzi were cloned. tzcrk3 encodes a 35 kDa protein sharing 51.5% amino acid identity with human cdc2 and 82% identity with Trypanosoma brucei CRK3. tzcrk1 encodes a 33 kDa protein sharing 52.7% identity with human cdc2 and a high degree of identity (>78%) with T. brucei CRK1, Leishmania mexicana CRK1 and Trypanosoma congolense CRK1. A recombinant TzCRK1 protein was able to phosphorylate histone HI and retinoblastoma protein. Western blotting using a polyclonal antibody raised against the recombinant TzCRK1 protein showed that the kinase is present in all life cycle stages of the parasite. A PSTAIRE antiserum detected proteins of 32, 33 and 35 kDa, with differential expression in the life cycle of the parasite. Transfection of COS-7 cells with tzcrk1 demonstrated for the first time that a CRK protein can bind mammalian cyclins; TzCRK1 co-immunoprecipitated with cyclins E, D3 and A suggesting a role for this kinase in cell cycle control. These results indicate that T. cruzi might have cyclin homologues that control the activity of the CRK proteins and that a complex mechanism would exist in order to regulate the kinases involved in the cell cycle and the differentiation processes of the parasite. Copyright (C) 1998 Elsevier Science B.V. Fil:Gómez, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v91_n2_p337_Gomez http://hdl.handle.net/20.500.12110/paper_01666851_v91_n2_p337_Gomez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cdc2-related protein kinase Cell cycle control Mammalian cyclins Trypanosoma cruzi cycline protein kinase amino acid sequence article cell cycle enzyme analysis gene sequence immunoblotting molecular cloning nonhuman nucleotide sequence priority journal protein analysis trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Blotting, Southern CDC2 Protein Kinase Cloning, Molecular COS Cells Cyclins Genes, Protozoan Genetic Complementation Test Histones Humans Molecular Probe Techniques Molecular Sequence Data Protein Kinases Protozoan Proteins Recombinant Proteins Retinoblastoma Protein Sequence Alignment Transfection Trypanosoma cruzi Leishmania mexicana Mammalia Protozoa Trypanosoma Trypanosoma brucei Trypanosoma congolense Trypanosoma cruzi
spellingShingle	cdc2-related protein kinase Cell cycle control Mammalian cyclins Trypanosoma cruzi cycline protein kinase amino acid sequence article cell cycle enzyme analysis gene sequence immunoblotting molecular cloning nonhuman nucleotide sequence priority journal protein analysis trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Blotting, Southern CDC2 Protein Kinase Cloning, Molecular COS Cells Cyclins Genes, Protozoan Genetic Complementation Test Histones Humans Molecular Probe Techniques Molecular Sequence Data Protein Kinases Protozoan Proteins Recombinant Proteins Retinoblastoma Protein Sequence Alignment Transfection Trypanosoma cruzi Leishmania mexicana Mammalia Protozoa Trypanosoma Trypanosoma brucei Trypanosoma congolense Trypanosoma cruzi Gómez, Eliana Beatriz Kornblihtt, Alberto Rodolfo Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins
topic_facet	cdc2-related protein kinase Cell cycle control Mammalian cyclins Trypanosoma cruzi cycline protein kinase amino acid sequence article cell cycle enzyme analysis gene sequence immunoblotting molecular cloning nonhuman nucleotide sequence priority journal protein analysis trypanosoma cruzi Amino Acid Sequence Animals Base Sequence Blotting, Southern CDC2 Protein Kinase Cloning, Molecular COS Cells Cyclins Genes, Protozoan Genetic Complementation Test Histones Humans Molecular Probe Techniques Molecular Sequence Data Protein Kinases Protozoan Proteins Recombinant Proteins Retinoblastoma Protein Sequence Alignment Transfection Trypanosoma cruzi Leishmania mexicana Mammalia Protozoa Trypanosoma Trypanosoma brucei Trypanosoma congolense Trypanosoma cruzi
description	Two cdc2-related protein kinases (crk), tzcrk3 and tzcrk1, from the protozoan parasite Trypanosoma cruzi were cloned. tzcrk3 encodes a 35 kDa protein sharing 51.5% amino acid identity with human cdc2 and 82% identity with Trypanosoma brucei CRK3. tzcrk1 encodes a 33 kDa protein sharing 52.7% identity with human cdc2 and a high degree of identity (>78%) with T. brucei CRK1, Leishmania mexicana CRK1 and Trypanosoma congolense CRK1. A recombinant TzCRK1 protein was able to phosphorylate histone HI and retinoblastoma protein. Western blotting using a polyclonal antibody raised against the recombinant TzCRK1 protein showed that the kinase is present in all life cycle stages of the parasite. A PSTAIRE antiserum detected proteins of 32, 33 and 35 kDa, with differential expression in the life cycle of the parasite. Transfection of COS-7 cells with tzcrk1 demonstrated for the first time that a CRK protein can bind mammalian cyclins; TzCRK1 co-immunoprecipitated with cyclins E, D3 and A suggesting a role for this kinase in cell cycle control. These results indicate that T. cruzi might have cyclin homologues that control the activity of the CRK proteins and that a complex mechanism would exist in order to regulate the kinases involved in the cell cycle and the differentiation processes of the parasite. Copyright (C) 1998 Elsevier Science B.V.
author	Gómez, Eliana Beatriz Kornblihtt, Alberto Rodolfo
author_facet	Gómez, Eliana Beatriz Kornblihtt, Alberto Rodolfo
author_sort	Gómez, Eliana Beatriz
title	Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins
title_short	Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins
title_full	Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins
title_fullStr	Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins
title_full_unstemmed	Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins
title_sort	cloning of a cdc2-related protein kinase from trypanosoma cruzi that interacts with mammalian cyclins
publishDate	1998
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v91_n2_p337_Gomez http://hdl.handle.net/20.500.12110/paper_01666851_v91_n2_p337_Gomez
work_keys_str_mv	AT gomezelianabeatriz cloningofacdc2relatedproteinkinasefromtrypanosomacruzithatinteractswithmammaliancyclins AT kornblihttalbertorodolfo cloningofacdc2relatedproteinkinasefromtrypanosomacruzithatinteractswithmammaliancyclins
_version_	1768543128935989248

Cloning of a cdc2-related protein kinase from Trypanosoma cruzi that interacts with mammalian cyclins

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