The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes

We demonstrate that cruzipain, the major cysteine proteinase of Trypanosoma cruzi epimastigotes, is encoded by a large number of tandemly arranged genes. Restriction enzyme analysis of 20 clones containing complete repeat units of the gene, as well as sequencing of 2 of these clones, and comparison...

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Autor principal:	Campetella, Oscar Eduardo
Publicado:	1992
Materias:	Chromosomal localization Cruzipain Cysteine proteinase Tandemly repeated genes Trypanosoma cruzi cysteine proteinase amino acid sequence animal cell article chromosome epimastigote genetic analysis immunoblotting nonhuman priority journal trypanosoma cruzi Amino Acid Sequence Animal Base Sequence Blotting, Northern Blotting, Southern Chromosome Mapping Cloning, Molecular Cysteine Endopeptidases DNA DNA Restriction Enzymes Electrophoresis, Gel, Pulsed-Field Molecular Sequence Data Multigene Family Polymorphism (Genetics) Repetitive Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Animalia Trypanosoma Trypanosoma brucei
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v50_n2_p225_Campetella http://hdl.handle.net/20.500.12110/paper_01666851_v50_n2_p225_Campetella
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_01666851_v50_n2_p225_Campetella
record_format	dspace
spelling	paper:paper_01666851_v50_n2_p225_Campetella2023-06-08T15:16:06Z The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes Campetella, Oscar Eduardo Chromosomal localization Cruzipain Cysteine proteinase Tandemly repeated genes Trypanosoma cruzi cysteine proteinase amino acid sequence animal cell article chromosome epimastigote genetic analysis immunoblotting nonhuman priority journal trypanosoma cruzi Amino Acid Sequence Animal Base Sequence Blotting, Northern Blotting, Southern Chromosome Mapping Cloning, Molecular Cysteine Endopeptidases DNA DNA Restriction Enzymes Electrophoresis, Gel, Pulsed-Field Molecular Sequence Data Multigene Family Polymorphism (Genetics) Repetitive Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Trypanosoma Trypanosoma brucei Trypanosoma cruzi We demonstrate that cruzipain, the major cysteine proteinase of Trypanosoma cruzi epimastigotes, is encoded by a large number of tandemly arranged genes. Restriction enzyme analysis of 20 clones containing complete repeat units of the gene, as well as sequencing of 2 of these clones, and comparison with previously published partial sequences, indicated that the sequence is conserved among the repeat units, although polymorphisms clearly exist. The repeat units contain an intergenic region of 528 bp and coding regions for pre- and pro-enzyme, a central domain and a C-terminal extension. The predicted amino acid sequences of these regions indicated a sequence identity of 30, 60, 70 and 36%, respectively, when the T. cruzi sequence was compared with the sequence of a similar cysteine proteinase from Trypanosoma brucei [14]. Studies by pulsed field gel electrophoresis, complemented with restriction analysis, indicated that the clusters are located on 2 4 different chromosomes in several parasite isolates. © 1992. Fil:Campetella, O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v50_n2_p225_Campetella http://hdl.handle.net/20.500.12110/paper_01666851_v50_n2_p225_Campetella
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Chromosomal localization Cruzipain Cysteine proteinase Tandemly repeated genes Trypanosoma cruzi cysteine proteinase amino acid sequence animal cell article chromosome epimastigote genetic analysis immunoblotting nonhuman priority journal trypanosoma cruzi Amino Acid Sequence Animal Base Sequence Blotting, Northern Blotting, Southern Chromosome Mapping Cloning, Molecular Cysteine Endopeptidases DNA DNA Restriction Enzymes Electrophoresis, Gel, Pulsed-Field Molecular Sequence Data Multigene Family Polymorphism (Genetics) Repetitive Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Trypanosoma Trypanosoma brucei Trypanosoma cruzi
spellingShingle	Chromosomal localization Cruzipain Cysteine proteinase Tandemly repeated genes Trypanosoma cruzi cysteine proteinase amino acid sequence animal cell article chromosome epimastigote genetic analysis immunoblotting nonhuman priority journal trypanosoma cruzi Amino Acid Sequence Animal Base Sequence Blotting, Northern Blotting, Southern Chromosome Mapping Cloning, Molecular Cysteine Endopeptidases DNA DNA Restriction Enzymes Electrophoresis, Gel, Pulsed-Field Molecular Sequence Data Multigene Family Polymorphism (Genetics) Repetitive Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Trypanosoma Trypanosoma brucei Trypanosoma cruzi Campetella, Oscar Eduardo The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes
topic_facet	Chromosomal localization Cruzipain Cysteine proteinase Tandemly repeated genes Trypanosoma cruzi cysteine proteinase amino acid sequence animal cell article chromosome epimastigote genetic analysis immunoblotting nonhuman priority journal trypanosoma cruzi Amino Acid Sequence Animal Base Sequence Blotting, Northern Blotting, Southern Chromosome Mapping Cloning, Molecular Cysteine Endopeptidases DNA DNA Restriction Enzymes Electrophoresis, Gel, Pulsed-Field Molecular Sequence Data Multigene Family Polymorphism (Genetics) Repetitive Sequences, Nucleic Acid RNA, Messenger Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Trypanosoma Trypanosoma brucei Trypanosoma cruzi
description	We demonstrate that cruzipain, the major cysteine proteinase of Trypanosoma cruzi epimastigotes, is encoded by a large number of tandemly arranged genes. Restriction enzyme analysis of 20 clones containing complete repeat units of the gene, as well as sequencing of 2 of these clones, and comparison with previously published partial sequences, indicated that the sequence is conserved among the repeat units, although polymorphisms clearly exist. The repeat units contain an intergenic region of 528 bp and coding regions for pre- and pro-enzyme, a central domain and a C-terminal extension. The predicted amino acid sequences of these regions indicated a sequence identity of 30, 60, 70 and 36%, respectively, when the T. cruzi sequence was compared with the sequence of a similar cysteine proteinase from Trypanosoma brucei [14]. Studies by pulsed field gel electrophoresis, complemented with restriction analysis, indicated that the clusters are located on 2 4 different chromosomes in several parasite isolates. © 1992.
author	Campetella, Oscar Eduardo
author_facet	Campetella, Oscar Eduardo
author_sort	Campetella, Oscar Eduardo
title	The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes
title_short	The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes
title_full	The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes
title_fullStr	The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes
title_full_unstemmed	The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes
title_sort	major cysteine proteinase (cruzipain) from trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes
publishDate	1992
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v50_n2_p225_Campetella http://hdl.handle.net/20.500.12110/paper_01666851_v50_n2_p225_Campetella
work_keys_str_mv	AT campetellaoscareduardo themajorcysteineproteinasecruzipainfromtrypanosomacruziisencodedbymultiplepolymorphictandemlyorganizedgeneslocatedondifferentchromosomes AT campetellaoscareduardo majorcysteineproteinasecruzipainfromtrypanosomacruziisencodedbymultiplepolymorphictandemlyorganizedgeneslocatedondifferentchromosomes
_version_	1768545183745441792

The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is encoded by multiple polymorphic tandemly organized genes located on different chromosomes

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