The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units
The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [ 14 C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding t...
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1990
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v39_n1_p101_Couto http://hdl.handle.net/20.500.12110/paper_01666851_v39_n1_p101_Couto |
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paper:paper_01666851_v39_n1_p101_Couto2023-06-08T15:16:04Z The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units Couto, Alicia Susana Muchnik de Lederkremer, Rosa María Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Protozoa Triticum aestivum Trypanosoma Trypanosoma cruzi The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [ 14 C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding to the lectin was lost on treatment of Tc-85 with neuraminidase. The N-linked asialo-oligosaccharide was released by endo-β-N-acetylglucosaminidase F digestion of asialo-Tc-85 and was further analyzed using specific exoglycosidases. [ 14 C]fucose was detected after α-l-fucosidase treatment or mild acid hydrolysis. The afucosyl oligosaccharide was 3 H-labeled by the galactose oxidase-NaB 3 H 4 method. [ 3 H]Galactose was released by α-galactosidase, and only then was β-galactosidase effective in removing another galactose. The gal(α1-3)gal unit was demonstrated by periodate oxidation studies on the [ 3 H]galactose-labeled asialo-glycoprotein. The presence of gal(α1-3)gal in Tc-85 could be related to the recent finding of elevated antibody levels against this epitope in patients with Chagas' disease. © 1990. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v39_n1_p101_Couto http://hdl.handle.net/20.500.12110/paper_01666851_v39_n1_p101_Couto |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Protozoa Triticum aestivum Trypanosoma Trypanosoma cruzi |
spellingShingle |
Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Protozoa Triticum aestivum Trypanosoma Trypanosoma cruzi Couto, Alicia Susana Muchnik de Lederkremer, Rosa María The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units |
topic_facet |
Gal(α1-3)gal Glycoprotein Tc-85 Trypanosoma cruzi epitope membrane antigen animal cell article chromatography glycoprotein structure nonhuman priority journal protozoon trypanosoma cruzi trypomastigote Acetylglucosaminidase Animal Disaccharides Fucose Glycoproteins Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Molecular Structure Protozoan Proteins Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Animalia Protozoa Triticum aestivum Trypanosoma Trypanosoma cruzi |
description |
The structure of the N-linked oligosaccharide of the 85-kDa surface glycoprotein (Tc-85) from the infective trypomastigote form of Trypanosoma cruzi was investigated. Tc-85 metabolically labeled with [ 14 C]glucose was purified by affinity chromatography on wheat germ agglutinin-Sepharose. Binding to the lectin was lost on treatment of Tc-85 with neuraminidase. The N-linked asialo-oligosaccharide was released by endo-β-N-acetylglucosaminidase F digestion of asialo-Tc-85 and was further analyzed using specific exoglycosidases. [ 14 C]fucose was detected after α-l-fucosidase treatment or mild acid hydrolysis. The afucosyl oligosaccharide was 3 H-labeled by the galactose oxidase-NaB 3 H 4 method. [ 3 H]Galactose was released by α-galactosidase, and only then was β-galactosidase effective in removing another galactose. The gal(α1-3)gal unit was demonstrated by periodate oxidation studies on the [ 3 H]galactose-labeled asialo-glycoprotein. The presence of gal(α1-3)gal in Tc-85 could be related to the recent finding of elevated antibody levels against this epitope in patients with Chagas' disease. © 1990. |
author |
Couto, Alicia Susana Muchnik de Lederkremer, Rosa María |
author_facet |
Couto, Alicia Susana Muchnik de Lederkremer, Rosa María |
author_sort |
Couto, Alicia Susana |
title |
The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units |
title_short |
The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units |
title_full |
The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units |
title_fullStr |
The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units |
title_full_unstemmed |
The N-linked carbohydrate chain of the 85-kilodalton glycoprotein from Trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units |
title_sort |
n-linked carbohydrate chain of the 85-kilodalton glycoprotein from trypanosoma cruzi trypomastigotes contains sialyl, fucosyl and galactosyl (α1-3)galactose units |
publishDate |
1990 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v39_n1_p101_Couto http://hdl.handle.net/20.500.12110/paper_01666851_v39_n1_p101_Couto |
work_keys_str_mv |
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_version_ |
1768543030534471680 |