Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization

A protein kinase C activity from epimastigote forms of Trypanosoma cruzi was characterized. Cytosolic extracts were chromatographed on DEAE-cellulose columns giving two peaks of kinase activity which were eluted at 0.1 and 0.15 M NaCl. The first activity peak requires Ca2+ and phosphatidylserine for...

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Detalles Bibliográficos
Autores principales: Gómez, María Laura, Erijman, Leonardo, Torres, Héctor Norberto
Publicado: 1989
Materias:
Diacylglycerol
Phorbol ester
Protein kinase C
Staurosporine
Trypanosoma cruzi
protein kinase c
animal cell
enzyme activity
epimastigote
nonhuman
protozoon
trypanosoma cruzi
Animal
Calcium
Chromatography, Agarose
Chromatography, DEAE-Cellulose
Cytosol
Diglycerides
Egtazic Acid
Histones
Microsomes
Phosphatidylserines
Phosphorylation
Protein Kinase C
Support, Non-U.S. Gov't
Tetradecanoylphorbol Acetate
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v36_n2_p101_Gomez
http://hdl.handle.net/20.500.12110/paper_01666851_v36_n2_p101_Gomez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_01666851_v36_n2_p101_Gomez
record_format dspace
spelling paper:paper_01666851_v36_n2_p101_Gomez2023-06-08T15:16:03Z Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization Gómez, María Laura Erijman, Leonardo Torres, Héctor Norberto Diacylglycerol Phorbol ester Protein kinase C Staurosporine Trypanosoma cruzi protein kinase c animal cell enzyme activity epimastigote nonhuman protozoon trypanosoma cruzi Animal Calcium Chromatography, Agarose Chromatography, DEAE-Cellulose Cytosol Diglycerides Egtazic Acid Histones Microsomes Phosphatidylserines Phosphorylation Protein Kinase C Support, Non-U.S. Gov't Tetradecanoylphorbol Acetate Trypanosoma cruzi A protein kinase C activity from epimastigote forms of Trypanosoma cruzi was characterized. Cytosolic extracts were chromatographed on DEAE-cellulose columns giving two peaks of kinase activity which were eluted at 0.1 and 0.15 M NaCl. The first activity peak requires Ca2+ and phosphatidylserine for activity. Further kinase purification was performed by chromatography on phenyl Sepharose columns. In these columns the enzyme activity was adsorbed in the presence of Ca2+ and eluted with a EGTA-containing buffer. T. cruzi protein kinase C activity preferentially phosphorylated histone H1. It was stimulated by diacylglycerol and phorbol myristate acetate, and inhibited by polymyxin B and staurosporine. After subcellular fractionation of epimastigote cells, the kinase was found to be associated with microsomal and cytosolic fractions. © 1989. Fil:Gomez, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Erijman, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v36_n2_p101_Gomez http://hdl.handle.net/20.500.12110/paper_01666851_v36_n2_p101_Gomez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Diacylglycerol
Phorbol ester
Protein kinase C
Staurosporine
Trypanosoma cruzi
protein kinase c
animal cell
enzyme activity
epimastigote
nonhuman
protozoon
trypanosoma cruzi
Animal
Calcium
Chromatography, Agarose
Chromatography, DEAE-Cellulose
Cytosol
Diglycerides
Egtazic Acid
Histones
Microsomes
Phosphatidylserines
Phosphorylation
Protein Kinase C
Support, Non-U.S. Gov't
Tetradecanoylphorbol Acetate
Trypanosoma cruzi
spellingShingle Diacylglycerol
Phorbol ester
Protein kinase C
Staurosporine
Trypanosoma cruzi
protein kinase c
animal cell
enzyme activity
epimastigote
nonhuman
protozoon
trypanosoma cruzi
Animal
Calcium
Chromatography, Agarose
Chromatography, DEAE-Cellulose
Cytosol
Diglycerides
Egtazic Acid
Histones
Microsomes
Phosphatidylserines
Phosphorylation
Protein Kinase C
Support, Non-U.S. Gov't
Tetradecanoylphorbol Acetate
Trypanosoma cruzi
Gómez, María Laura
Erijman, Leonardo
Torres, Héctor Norberto
Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization
topic_facet Diacylglycerol
Phorbol ester
Protein kinase C
Staurosporine
Trypanosoma cruzi
protein kinase c
animal cell
enzyme activity
epimastigote
nonhuman
protozoon
trypanosoma cruzi
Animal
Calcium
Chromatography, Agarose
Chromatography, DEAE-Cellulose
Cytosol
Diglycerides
Egtazic Acid
Histones
Microsomes
Phosphatidylserines
Phosphorylation
Protein Kinase C
Support, Non-U.S. Gov't
Tetradecanoylphorbol Acetate
Trypanosoma cruzi
description A protein kinase C activity from epimastigote forms of Trypanosoma cruzi was characterized. Cytosolic extracts were chromatographed on DEAE-cellulose columns giving two peaks of kinase activity which were eluted at 0.1 and 0.15 M NaCl. The first activity peak requires Ca2+ and phosphatidylserine for activity. Further kinase purification was performed by chromatography on phenyl Sepharose columns. In these columns the enzyme activity was adsorbed in the presence of Ca2+ and eluted with a EGTA-containing buffer. T. cruzi protein kinase C activity preferentially phosphorylated histone H1. It was stimulated by diacylglycerol and phorbol myristate acetate, and inhibited by polymyxin B and staurosporine. After subcellular fractionation of epimastigote cells, the kinase was found to be associated with microsomal and cytosolic fractions. © 1989.
author Gómez, María Laura
Erijman, Leonardo
Torres, Héctor Norberto
author_facet Gómez, María Laura
Erijman, Leonardo
Torres, Héctor Norberto
author_sort Gómez, María Laura
title Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization
title_short Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization
title_full Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization
title_fullStr Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization
title_full_unstemmed Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization
title_sort protein kinase c in trypanosoma cruzi epimastigote forms: partial purification and characterization
publishDate 1989
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v36_n2_p101_Gomez
http://hdl.handle.net/20.500.12110/paper_01666851_v36_n2_p101_Gomez
work_keys_str_mv AT gomezmarialaura proteinkinasecintrypanosomacruziepimastigoteformspartialpurificationandcharacterization
AT erijmanleonardo proteinkinasecintrypanosomacruziepimastigoteformspartialpurificationandcharacterization
AT torreshectornorberto proteinkinasecintrypanosomacruziepimastigoteformspartialpurificationandcharacterization
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