Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi
The carbohydrate moiety of the Tc-85 surface glycoprotein from the infective trypomastigote form of Trypanosoma cruzi was analysed. Tc-85 could be metabolically labeled by incubation of the cells with d-[14C]mannose or d-[14C]glucose. Degradation techniques were performed directly on the polyacrylam...
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1987
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v26_n1-2_p145_Couto http://hdl.handle.net/20.500.12110/paper_01666851_v26_n1-2_p145_Couto |
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paper:paper_01666851_v26_n1-2_p145_Couto2023-06-08T15:16:03Z Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi Couto, Alicia Susana Muchnik de Lederkremer, Rosa María Glycoprotein carbohydrate composition Sialic acid Trypanosoma cruzi Trypomastigote glycoprotein membrane protein n acetylneuraminic acid oligosaccharide sialic acid derivative affinity chromatography animal article gel chromatography paper chromatography paper electrophoresis Trypanosoma cruzi Animal Chromatography, Affinity Chromatography, Gel Chromatography, Paper Electrophoresis, Paper Glycoproteins Membrane Proteins N-Acetylneuraminic Acid Oligosaccharides Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi The carbohydrate moiety of the Tc-85 surface glycoprotein from the infective trypomastigote form of Trypanosoma cruzi was analysed. Tc-85 could be metabolically labeled by incubation of the cells with d-[14C]mannose or d-[14C]glucose. Degradation techniques were performed directly on the polyacrylamide gel band containing labeled Tc-85. A mannobiose was cleaved by β-elimination and further treatment of the remaining material under conditions which liberate N-asparaginyl linkages, released a complex oligosaccharide. The presence of sialic acid was demonstrated by: mild acid hydrolysis, neuraminidase treatment and periodate oxidation under mild conditions followed by NaB3H4 reduction, hydrolysis, and detection of NANA7 by paper electrophoresis. In addition, the chromatographic behavior of the asialooligosaccharide was significantly different from that of the original sample. Galactose, mannose and glucosamine are the other monosaccharide components of the sialooligosaccharide. © 1987. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v26_n1-2_p145_Couto http://hdl.handle.net/20.500.12110/paper_01666851_v26_n1-2_p145_Couto |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Glycoprotein carbohydrate composition Sialic acid Trypanosoma cruzi Trypomastigote glycoprotein membrane protein n acetylneuraminic acid oligosaccharide sialic acid derivative affinity chromatography animal article gel chromatography paper chromatography paper electrophoresis Trypanosoma cruzi Animal Chromatography, Affinity Chromatography, Gel Chromatography, Paper Electrophoresis, Paper Glycoproteins Membrane Proteins N-Acetylneuraminic Acid Oligosaccharides Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi |
spellingShingle |
Glycoprotein carbohydrate composition Sialic acid Trypanosoma cruzi Trypomastigote glycoprotein membrane protein n acetylneuraminic acid oligosaccharide sialic acid derivative affinity chromatography animal article gel chromatography paper chromatography paper electrophoresis Trypanosoma cruzi Animal Chromatography, Affinity Chromatography, Gel Chromatography, Paper Electrophoresis, Paper Glycoproteins Membrane Proteins N-Acetylneuraminic Acid Oligosaccharides Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi Couto, Alicia Susana Muchnik de Lederkremer, Rosa María Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi |
topic_facet |
Glycoprotein carbohydrate composition Sialic acid Trypanosoma cruzi Trypomastigote glycoprotein membrane protein n acetylneuraminic acid oligosaccharide sialic acid derivative affinity chromatography animal article gel chromatography paper chromatography paper electrophoresis Trypanosoma cruzi Animal Chromatography, Affinity Chromatography, Gel Chromatography, Paper Electrophoresis, Paper Glycoproteins Membrane Proteins N-Acetylneuraminic Acid Oligosaccharides Sialic Acids Support, Non-U.S. Gov't Trypanosoma cruzi |
description |
The carbohydrate moiety of the Tc-85 surface glycoprotein from the infective trypomastigote form of Trypanosoma cruzi was analysed. Tc-85 could be metabolically labeled by incubation of the cells with d-[14C]mannose or d-[14C]glucose. Degradation techniques were performed directly on the polyacrylamide gel band containing labeled Tc-85. A mannobiose was cleaved by β-elimination and further treatment of the remaining material under conditions which liberate N-asparaginyl linkages, released a complex oligosaccharide. The presence of sialic acid was demonstrated by: mild acid hydrolysis, neuraminidase treatment and periodate oxidation under mild conditions followed by NaB3H4 reduction, hydrolysis, and detection of NANA7 by paper electrophoresis. In addition, the chromatographic behavior of the asialooligosaccharide was significantly different from that of the original sample. Galactose, mannose and glucosamine are the other monosaccharide components of the sialooligosaccharide. © 1987. |
author |
Couto, Alicia Susana Muchnik de Lederkremer, Rosa María |
author_facet |
Couto, Alicia Susana Muchnik de Lederkremer, Rosa María |
author_sort |
Couto, Alicia Susana |
title |
Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi |
title_short |
Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi |
title_full |
Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi |
title_fullStr |
Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi |
title_full_unstemmed |
Sialic acid in a complex oligosaccharide chain of the Tc-85 surface glycoprotein from the trypomastigote stage of Trypanosoma cruzi |
title_sort |
sialic acid in a complex oligosaccharide chain of the tc-85 surface glycoprotein from the trypomastigote stage of trypanosoma cruzi |
publishDate |
1987 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v26_n1-2_p145_Couto http://hdl.handle.net/20.500.12110/paper_01666851_v26_n1-2_p145_Couto |
work_keys_str_mv |
AT coutoaliciasusana sialicacidinacomplexoligosaccharidechainofthetc85surfaceglycoproteinfromthetrypomastigotestageoftrypanosomacruzi AT muchnikdelederkremerrosamaria sialicacidinacomplexoligosaccharidechainofthetc85surfaceglycoproteinfromthetrypomastigotestageoftrypanosomacruzi |
_version_ |
1768544906397089792 |